| 质粒名称: | pT3-N90-beta-catenin |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31785 |
| 存储实验室: | Xin Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pT3-EF1a |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | beta-catenin,b-catenin,CTNNB1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2121 bp |
| 基因突变: | Deletion of aa 1-90- Constitutitvely Active |
| GenBank编号 | -- |
| 启动子 | EF1alpha |
| 标签/融合蛋白: | Myc（插入件上的N端子） |
| 克隆方法: | Gateway 克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | EF-1a-Fwd |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pT3-N90-beta-catenin was a gift from Xin Chen (Addgene plasmid # 31785 ; http://n2t.net/addgene:31785 ; RRID:Addgene_31785) |
| 参考文献部分: | Distinct pathways of genomic progression to benign and malignant tumors of the liver. Tward AD, Jones KD, Yant S, Cheung ST, Fan ST, Chen X, Kay MA, Wang R, Bishop JM. Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep 4. 10.1073/pnas.0706578104 PubMed 17785413 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pT3-N90-beta-catenin 7681 bp ds-DNA circular SYN 26-MAR-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pT3-N90-beta-catenin
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7681)
AUTHORS Tward AD, Jones KD, Yant S, Cheung ST, Fan ST, Chen X, Kay MA, Wang
R, Bishop JM
TITLE Distinct pathways of genomic progression to benign and malignant
tumors of the liver.
JOURNAL Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep
4.
PUBMED 17785413
REFERENCE 2 (bases 1 to 7681)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 26, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7681
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 90..1268
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 321..1259
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1216..1236
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind 1285..1304
/label=T7
/note="T7 promoter, forward primer"
promoter 1285..1303
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1382..1406
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 1417..1446
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
protein_bind complement(3536..3560)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 3613..3654
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
primer_bind complement(3692..3709)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3698..3922
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind complement(3992..4025)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind 4081..4101
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
primer_bind complement(4483..4500)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(4483..4499)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4492..4514)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(4708..4727)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4827..4849
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4887..4905)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4973..5077
/gene="bla"
/label=AmpR promoter
CDS 5078..5938
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5296..5315)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6109..6697
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6598..6617
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6851..6868
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 6985..7006
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7021..7051
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7059..7075
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7064..7086
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 7083..7099
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7083..7099
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7525..7544)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
protein_bind complement(7597..7630)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
ORIGIN
1 aaaaagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
61 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
121 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
181 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
241 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
301 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
361 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
421 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
481 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
541 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
601 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
661 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
721 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
781 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
841 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
901 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
961 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
1021 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
1081 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
1141 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
1201 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
1261 tgtcgtgagg aattagcttg gtactaatac gactcactat agggagaccc aagctggcta
1321 ggtaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc
1381 aacaagtttg tacaaaaaag caggcttcgt accatggagc agaagctgat ctccgaggag
1441 gacctgaagg atcctcgagc tcagagggta cgagctgcta tgttccctga gacattagat
1501 gagggcatgc agatcccatc tacacagttt gatgctgctc atcccactaa tgtccagcgt
1561 ttggctgaac catcacagat gctgaaacat gcagttgtaa acttgattaa ctatcaagat
1621 gatgcagaac ttgccacacg tgcaatccct gaactgacaa aactgctaaa tgacgaggac
1681 caggtggtgg ttaataaggc tgcagttatg gtccatcagc tttctaaaaa ggaagcttcc
1741 agacacgcta tcatgcgttc tcctcagatg gtgtctgcta ttgtacgtac catgcagaat
1801 acaaatgatg tagaaacagc tcgttgtacc gctgggacct tgcataacct ttcccatcat
1861 cgtgagggct tactggccat ctttaagtct ggaggcattc ctgccctggt gaaaatgctt
1921 ggttcaccag tggattctgt gttgttttat gccattacaa ctctccacaa ccttttatta
1981 catcaagaag gagctaaaat ggcagtgcgt ttagctggtg ggctgcagaa aatggttgcc
2041 ttgctcaaca aaacaaatgt taaattcttg gctattacga cagactgcct tcaaatttta
2101 gcttatggca accaagaaag caagctcatc atactggcta gtggtggacc ccaagcttta
2161 gtaaatataa tgaggaccta tacttacgaa aaactactgt ggaccacaag cagagtgctg
2221 aaggtgctat ctgtctgctc tagtaataag ccggctattg tagaagctgg tggaatgcaa
2281 gctttaggac ttcacctgac agatccaagt caacgtcttg ttcagaactg tctttggact
2341 ctcaggaatc tttcagatgc tgcaactaaa caggaaggga tggaaggtct ccttgggact
2401 cttgttcagc ttctgggttc agatgatata aatgtggtca cctgtgcagc tggaattctt
2461 tctaacctca cttgcaataa ttataagaac aagatgatgg tctgccaagt gggtggtata
2521 gaggctcttg tgcgtactgt ccttcgggct ggtgacaggg aagacatcac tgagcctgcc
2581 atctgtgctc ttcgtcatct gaccagccga caccaagaag cagagatggc ccagaatgca
2641 gttcgccttc actatggact accagttgtg gttaagctct tacacccacc atcccactgg
2701 cctctgataa aggctactgt tggattgatt cgaaatcttg ccctttgtcc cgcaaatcat
2761 gcacctttgc gtgagcaggg tgccattcca cgactagttc agttgcttgt tcgtgcacat
2821 caggataccc agcgccgtac gtccatgggt gggacacagc agcaatttgt ggagggggtc
2881 cgcatggaag aaatagttga aggttgtacc ggagcccttc acatcctagc tcgggatgtt
2941 cacaaccgaa ttgttatcag aggactaaat accattccat tgtttgtgca gctgctttat
3001 tctcccattg aaaacatcca aagagtagct gcaggggtcc tctgtgaact tgctcaggac
3061 aaggaagctg cagaagctat tgaagctgag ggagccacag ctcctctgac agagttactt
3121 cactctagga atgaaggtgt ggcgacatat gcagctgctg ttttgttccg aatgtctgag
3181 gacaagccac aagattacaa gaaacggctt tcagttgagc tgaccagctc tctcttcaga
3241 acagagccaa tggcttggaa tgagactgct gatcttggac ttgatattgg tgcccaggga
3301 gaaccccttg gatatcgcca ggatgatcct agctatcgtt cttttcactc tggtggatat
3361 ggccaggatg ccttgggtat ggaccccatg atggaacatg agatgggtgg ccaccaccct
3421 ggtgctgact atccagttga tgggctgcca gatctggggc atgcccagga cctcatggat
3481 gggctgcctc caggtgacag caatcagctg gcctggtttg atactgacct gtaagaccca
3541 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc
3601 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
3661 ggttagtaat gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
3721 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
3781 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
3841 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
3901 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
3961 tatccccacg cgccctgtag cggcgcatta aataacttcg tataatgtat gctatacgaa
4021 gttatatcgt atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
4081 accctaactg acacacattc cacagaattc cttgaaatac atccacaggt acacctccaa
4141 ttgactcaaa tgatgtcaat tagtctatca gaagcttcta aagccatgac atcattttct
4201 ggaattttcc aagctgttta aaggcacagt caacttagtg tatgtaaact tctgacccac
4261 tggaattgtg atacagtgaa ttataagtga aataatctgt ctgtaaacaa ttgttggaaa
4321 aatgacttgt gtcatgcaca aagtagatgt cctaactgac ttgccaaaac tattgtttgt
4381 taacaagaaa tttgtggagt agttgaaaaa cgagttttaa tgactccaac ttaagtgtat
4441 gtaaacttcc gacttcaact gtagggtacc gagctcgaat tcactggccg tcgttttaca
4501 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
4561 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
4621 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
4681 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
4741 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
4801 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
4861 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
4921 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
4981 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
5041 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
5101 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
5161 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
5221 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
5281 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
5341 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
5401 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
5461 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
5521 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
5581 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
5641 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
5701 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
5761 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
5821 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
5881 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
5941 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
6001 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
6061 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
6121 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
6181 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
6241 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
6301 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
6361 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
6421 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
6481 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
6541 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
6601 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
6661 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
6721 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
6781 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
6841 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
6901 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
6961 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
7021 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
7081 cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag
7141 acctatacag ttgaagtcgg aagtttacat acacttaagt tggagtcatt aaaactcgtt
7201 tttcaactac tccacaaatt tcttgttaac aaacaatagt tttggcaagt cagttaggac
7261 atctactttg tgcatgacac aagtcatttt tccaacaatt gtttacagac agattatttc
7321 acttataatt cactgtatca caattccagt gggtcagaag tttacataca ctaagttgac
7381 tgtgccttta aacagcttgg aaaattccag aaaatgatgt catggcttta gaagcttctg
7441 atagactaat tgacatcatt tgagtcaatt ggaggtgtac ctgtggatgt atttcaaggg
7501 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagcgg
7561 ccgctagctc gagatcttaa ttaaactagt atcgatataa cttcgtataa tgtatgctat
7621 acgaagttat atgtacgggc cagatatacg cgttgacatt gattattgac taggcttttg
7681 c
//
