| 出品公司: | ATCC |
|---|---|
| 载体名称: | YEp24(pRB5) |
| 产品编号: | 37051 |
| 生长条件： | 37°C 1 day(s) |
| 生物安全等级: | 1级 |
| 宿主菌: | 酿酒酵母大肠杆菌 |
| 载体类型: | 质粒 |
| 复制子: | 2 micron(S. cerevisiae), pMB1(E. coli) |
| 抗性: | 氨苄、四环素、URA3 |
| 启动子: | |
| 建议宿主菌: | rec-大肠杆菌 |
| 载体大小（kb）: | 7.8 |
| 多克隆位点: | 是 |
| 备注： |
叶型会阴穿梭向量。质粒是通过将2.2 kb ecori 2微米b片段和1.1 kb hindiii-ura3片段插入pbr322的ecori和hindiii位点来构建的。
|
| 参考文献: |
P251
Barnes, D. A., and J. Thorner. 1985. Use of the LYS2 gene for gene disruption, gene replacment and promoter analysis in Saccharomyces cerevisiae. In Gene Manipulation in Fungi. pp. 197-226.
P7
Botstein, D., S. C. Falco, S. E. Stewart, M. Brennan, S. Scherer, D. T. Stinchcomb, K. Struhl, and R. W. Davis. 1979. Sterile host yeasts (SHY) : a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24.
|
买家导航
