| 质粒名称: | IFN-Beta_pGL3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 102597 |
| 存储实验室: | Nicolas Manel |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Human IFN-Beta promoter |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | luciferase（主干上的C末端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | IFN-Beta_pGL3 was a gift from Nicolas Manel (Addgene plasmid # 102597 ; http://n2t.net/addgene:102597 ; RRID:Addgene_102597) |
| 参考文献部分: | Transmission of innate immune signaling by packaging of cGAMP in viral particles. Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N. Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628. Epub 2015 Jul 30. 10.1126/science.aab3628 PubMed 26229115 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS IFN-Beta_pGL3 5153 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION IFN-beta promoter driving luciferase. Use in IFN reporter assay..
ACCESSION .
VERSION .
KEYWORDS IFN-Beta_pGL3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5153)
AUTHORS Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M,
Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N
TITLE Transmission of innate immune signaling by packaging of cGAMP in
viral particles.
JOURNAL Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628.
Epub 2015 Jul 30.
PUBMED 26229115
REFERENCE 2 (bases 1 to 5153)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5153
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 660..2312
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(726..744)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 2353..2474
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2364..2383)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2418..2437
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2722..2739)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2893..3481)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2973..2992)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3652..4512)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4275..4294
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4513..4617)
/gene="bla"
/label=AmpR promoter
rep_origin 4644..5099
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4731..4750)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4941..4962
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 cttcgctatt acgccagaat tctcaggtcg tttgctttcc tttgctttct cccaagtctt
61 gttttacaat ttgctttagt cattcactga aattttaaaa aacattagaa aacctcacag
121 tttgtaaatc tttttcccta ttatatatat cataagatag gagcttaaat aaagagtttt
181 agaaactact aaaatgtaaa tgacatagga aaactgaaag ggagaagtga aagtgggaaa
241 ttcctctgaa tagagagagg accatctcat ataaataggc catacccatg gagaaaggac
301 attctaactg caacctttcg aattctcagg tcgtttgctt tcctttgctt tctcccaagt
361 cttgttttac aatttgcttt agtcattcac tgaaatttta aaaaacatta gaaaacctca
421 cagtttgtaa atctttttcc ctattatata tatcataaga taggagctta aataaagagt
481 tttagaaact actaaaatgt aaatgacata ggaaaactga aagggagaag tgaaagtggg
541 aaattcctct gaatagagag aggaccatct catataaata ggccataccc atggagaaag
601 gacattctaa ctgcaacctt tcgctagccc gggctcgaga tctgcgatct aagtaagcta
661 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
721 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
781 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
841 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
901 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
961 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt
1021 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
1081 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat
1141 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
1201 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat
1261 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc
1321 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
1381 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
1441 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca
1501 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga
1561 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta
1621 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg
1681 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
1741 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
1801 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt
1861 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
1921 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc
1981 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac
2041 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc
2101 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
2161 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
2221 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
2281 aggccaagaa gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc
2341 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
2401 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
2461 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
2521 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
2581 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
2641 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
2701 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
2761 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
2821 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
2881 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
2941 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
3001 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
3061 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
3121 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
3181 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
3241 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
3301 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
3361 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
3421 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
3481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
3541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
3601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
3661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
3721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
3781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
3841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
3901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
3961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
4021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
4081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
4141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
4201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
4261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
4321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
4381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
4441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
4501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
4561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
4621 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
4681 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
4741 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
4801 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
4861 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
4921 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
4981 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
5041 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg
5101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cct
//
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