| 质粒名称: | pAWP78-PVCpnf_pvc13-truncated |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 198286 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pAWP78 |
| 完整质粒大小: | 25130bp |
| 载体类型: | 细菌细胞表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PVCpnf_pvc13-truncated |
| 物种来源: | Photorhabdus asymbiotica |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: |
pAWP78-PVCpnf_pvc13-truncated was a gift from Feng Zhang (Addgene plasmid # 198286 ; http://n2t.net/addgene:198286 ; RRID:Addgene_198286)
|
| 参考文献部分: | Programmable protein delivery with a bacterial contractile injection system. Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. 10.1038/s41586-023-05870-7 PubMed 36991127 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAWP78-PVCpnf_pvc13-truncated 25130 bp ds-DNA circular SYN 27-MAR-2023
DEFINITION Contains a tail fiber with a truncated binding domain (lacking
aa403-476 in Pvc13); produces nontargeting PVCs.
ACCESSION .
VERSION .
KEYWORDS pAWP78-PVCpnf_pvc13-truncated
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 25130)
AUTHORS Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F
TITLE Programmable protein delivery with a bacterial contractile injection
system
JOURNAL Nature March 29, 2023
REFERENCE 2 (bases 1 to 25130)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 27, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..25130
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 27..46
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 111..130
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS complement(423..1571)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(1843..2214)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 2247..2356
/note="incP origin of transfer"
rep_origin 2509..3139
/label=oriV
/note="incP origin of replication"
rep_origin 3448..4036
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3937..3956
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(24434..119)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
1 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
61 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata
121 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
181 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc gacttccggc
241 aagctatacg cgccctagaa ttgtcaattt taatcctctg tttatcggca gttcgtagag
301 cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt
361 tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg
421 ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg
481 cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa
541 tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag
601 ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg
661 aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg
721 caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat
781 tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg
841 cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc
901 tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc
961 tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc
1021 acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg
1081 ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc
1141 ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt
1201 ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt
1261 tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct
1321 gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca
1381 gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag
1441 ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg
1501 gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac
1561 gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct
1621 tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg
1681 aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc
1741 tgcacgaata ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg
1801 gcaatcacgc gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg
1861 gcggaccacg cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag
1921 cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag
1981 tttcagcagg ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg
2041 ctcatagtcc acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccta
2101 caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca
2161 gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt
2221 gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag
2281 caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac
2341 ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct
2401 ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat
2461 gaccccgaag cagggttatg cagcggaaaa gatccgtcga ccctttccga cgctcaccgg
2521 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac
2581 gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa
2641 cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg
2701 cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc
2761 ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttt ccacagatga tgtggacaag
2821 cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg
2881 ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac
2941 atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt
3001 cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt
3061 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
3121 cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc
3181 cgcgaacggc ctcaccccaa aaatggcagc caagctgacc cgctagggac gtgaagtcga
3241 ttccttcgat ggttagcaat caaagactca gagtgctgtc tggagcgtga atctaacggt
3301 acgtatctcg attgctcggt cgctattcgc actctgcgaa agttcgtacc gctcattcac
3361 taggttgcga atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
3421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
3481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
3541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
3601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
3661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
3721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
3781 cacacagccc agcttgtagc gaacgaccta caccgaactg agatacctac agcgtgagct
3841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
3901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
3961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
4021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
4081 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
4141 cgcctttgag tgagctgtcg cgaatgcatc tagatataat tacatcttca tcattcggta
4201 ttttaaatga catatatcct ccttccgaat agttttaatg gtgatgttcg attggacagt
4261 gttttccatt attaaaattt taagtatcac atcatgttta atgaaggatt gatgtttatt
4321 agaatattca taatagcttt gtctttgcct ctatgtaaat gaaattgcat aaaaagcaaa
4381 ttgcattatt ggcaatgtac ttattctgga tttttcggca cgataaatta ttggtgtaag
4441 ttttatatca tgagtaaaaa ttcggaatga tattcctgtc attttgtggc ggtagcgtta
4501 ctcaagatat tatcctacat catatgttga ttattgtaat caatcgagct attttttgtc
4561 taaaaagggg cttttatgtc tacaagtaca tctcaaattg cggttgaata tcctattcct
4621 gtctatcgct ttattgtttc tgtcggagat gagaaaattc catttaatag tgtttcagga
4681 ttagatatta gttatgacac cattgaatac cgagatggtg ttggtaattg gttcaaaatg
4741 ccgggtcaga gtcagagcac taatatcacc ttgcgtaaag gcgttttccc ggggaaaaca
4801 gaactgtttg attggattaa ctctattcag cttaatcagg tagagaaaaa ggatattacc
4861 atcagtttaa ctaatgatgc aggtaccgaa ttattaatga cctggaatgt ttctaatgct
4921 tttcccactt cattgacttc accttcattt gatgccacca gtaatgatat tgcagtacag
4981 gaaattacgc tgatggcaga tcgggtgatt atgcaggctg tttgaagcat tgatatttaa
5041 tcatctcata taagggaact tttatgacaa ccgttaccag ttatcctggc gtttatattg
5101 aagaattaaa tagcctggcc ttgtcagttt caaatagcgc cacagcggtt cctgtttttg
5161 ctgtggacga acaaaaccaa tatattagtg aagataatgc aatccgtatt aattcgtgga
5221 tggattatct taatctgatt ggcaatttta ataatgaaga caaattagat gtttctgtgc
5281 gtgcttattt tgccaatgga ggtggatatt gttatctcgt caaaacaacg agtttagaaa
5341 aaattattcc aaccttggat gatgtaacct tattggttgc tgcgggcgaa gatattaaaa
5401 cgacagtaga tgttttatgt cagccaggaa aagggttatt cgcagtcttt gatggccctg
5461 aaacagagtt gactatcaac ggtgcggaag aggcaaaaca agcctatacc gccacaccat
5521 tcgctgcggt ttattatcct tggttgaaag cggattgggc taacatagat attccaccca
5581 gtgcagtgat ggcgggagtt tatgcatcgg tggatttatc ccgtggtgta tggaaagcgc
5641 ctgccaatgt tgcgttgaaa gggggcctgg aacctaaatt tttagtcacg gatgaattgc
5701 agggtgaata taacactggc cgcgctatca atatgattcg taatttcagt aacacaggta
5761 ctacggtttg gggtgcaaga accctggaag ataaagacaa ttggcgttat gttccagtgc
5821 gacgcttgtt taattctgtg gagcgggata tcaagcgtgc catgagcttt gctatgttcg
5881 agcctaataa tcagcctact tgggagcggg tacgggcggc gattagcaac tacctttata
5941 gcctgtggca acagggggga ttagctggca gcaaagaaga agacgcttat tttgtgcaaa
6001 ttggtaaagg tataacgatg acacaggagc agattgatgc agggcaaatg attgttaaag
6061 tcggtttggc tgctgtacgg cctgcggaat ttatcattct ccagtttacg caagatgtag
6121 aacagcgtta atcatatgat tatgaggagt tatcatgtct gctattctga aagcgcctgg
6181 cgtttatatt gaagaagacg cttccctagc gttgtctgtc agtaacagcg cgactgccgt
6241 gcctgttttt atcggaaaat ttactccgac agtggttgat tcaatccaag tctgtacccg
6301 tatcagcaac tggcttgaat tcacttcctc tttttcccta gctccaacag ttgagattgt
6361 tgtccaatct aacactgaat ctgaatctga atctgaaact taccactata ttgagacaat
6421 caatttatct ccagctgtgg aagcattgcg actctatttt caaaatggcg gaggagcttg
6481 ctatatctac ccattaaatg atgctgaaga tgaattggtt ctggcggcca taccagaagt
6541 cattgaacag aaaggtgata ttactctgtt ggtttgcccg gaactcgatc tggattacaa
6601 aactaagatc tatggcgcag tgagctcact gttgaatgat aacaaagtgg gctatttcct
6661 gattgcggat agcaatgatg gagaatctgt gtcaggagta tggaatagtg ctaaggccgc
6721 cgcctattat ccccagttgg aaactaacct aaaattttcc acgttgcctg gggataagga
6781 cattcgtatc agcggttatc aggatgatga tgaaacacat aaaccgaaaa acttggatga
6841 gctcaggaca atcaacgagg cgttggcaca ggatattgat gcaagattgc tcgaggagaa
6901 acaacgtgct gtcatcattc cgccaagtgc tgccattgcg ggcatttatt gccaaacgga
6961 taatcgtcgc ggtgtttgga aagcgccagc caacgttgcg ctcacaggga tcgggagttt
7021 gcttgataag gtagacgatg aacggcaggg agagatgaat gacaagggaa tcaatgtcat
7081 ccgttcattt accgaccgtg gttttatggt ctggggagcc cgtacttgtg tggacgctgc
7141 caacatcagc tggcgttata ttcctgttcg tcgcctgttc aattccgttg aacgagatat
7201 ccgccaggcg ctgcgcgctg tgttgtttga aactaatagt cagcctacct gggtacgtgc
7261 taaggctgcc gttgatcaat atctttatac cctttggcag aaaaatgcat tgatgggtgc
7321 tcgcccggaa gaagcttatt ttgtgcaaat tggtcaggat atcaccatgt ccgaggctga
7381 tattaaacag ggtaagatga tcatgactgt tggtttggca gcagtgcggc cagctgagtt
7441 catcattctg caatttacgc aggatgttgt tcagtaatct ccatgactaa acgccaggca
7501 ctgtattgac agtgcctact ctaaccatct tggaggaggt gatgatgatg gagagactcc
7561 aaccgggtgt gactttaaca gaaagtataa tcacgatggg tcagcaagag atacccagtg
7621 ctgtgccggt gtttattggt tacaccgttc gttatccgga acaatcggaa gcatcagtcc
7681 gtatcgacag tttggccgag tataccagcc tgtttggtga cgaccatgtg atgatgtttg
7741 ctgtcaggca ctattttgat aatggcgggc aacaggcatt tgttttaccc ctgaaggaca
7801 atatgccatc agtggagatg accacagctg aagcggaaaa tctgatagcc gcattgcgct
7861 ctgctacggt tagcgaagcc attggtgggc atagtcagat tacactgatt ttggtaccgg
7921 atatggctcg gcttaatgac agtgatattg atgactcctc aacccaggta agcctgtggt
7981 cccaaggctg ggaggcgctg ctgcaattga gtcaggttag gcccaacctc tttgtgctgt
8041 tagatgcgcc ggataatgtt gaacaggcgc agaagtgtat gacaacgcta tcgtcagatt
8101 atcgtcaatg gggggcagca tattggcctc gtctggaaac tacctatcag aaagaaatat
8161 ctggcaagga caatgaatct cagggaattt tccaggggac tgttctgtca cccacagccg
8221 cggtcgcagc ggtaattcaa cgcacggata acgacgcggg tgtttggaaa gcaccggcca
8281 atattgcctt atcccaggtt attcgacctg ttaaatctta tcttcaggga agtgtactgt
8341 ttaacagcag cggcacttcg ctcaatgtga tccgcagttt cccaggtaag ggcatacggg
8401 tatggggatg ccgcactctg gaaaacacgg ataatacgca gtggcgctat ctgcaaacac
8461 gtcggctggt ttcctatgta acagcgcatt tgacccaatt ggctcgcatg tatgtcttcg
8521 agccaaataa tgaacttacc tggatgaagt taaaaggaca aagttacaac tggttacggc
8581 aattatggtt gcagggtggc ttgtatggtt cacaggagga tgaggcattt aacattctgt
8641 taggcgtaaa cgagacgatg actgaggatg atgttcgtgc aggaaaaatg atcatgaaag
8701 ttgagttggc tgtgttgttt cctgccgaat ttattgagat cagtttggtg tttaataccc
8761 aaacagaggc gctgtcttaa gaaggaaaaa gtacgatgaa cgattattac acacccgtgg
8821 tatcccatcg ttttatggcg agttttattt ttaaccgcat tcccgatccg ctggatattc
8881 gttttcagcg tatctctggc cttagtcggg aactacaggt gactcagtac agtgagggag
8941 gagaaaatgc ccgtaataac tatttagctg agaaaatcca acacggtacg ttgactttgg
9001 aacggggcgt gatgacagtc tcgccattga cctggatgtt tgatcgggta ttgagtggtg
9061 aaaaaatcgc ttatgccgat gtggtggtga tgctactgaa tgaaaattca ctgccattgt
9121 ccagttggac gttgagcaat gcgctgccgg tacgctggca aaccagcgac tttgacgcta
9181 acagcaatgc catattggtg aatacccttg aattgcgtta ccaggatatg cgctggcttg
9241 gagtcaaaat atgacagtag aaatcagaga gttacttatc caggcaaagg tagtgccatc
9301 aacacgaccg actgaatcag aacggcaaaa ccattctttg atacaggaaa gtctggatga
9361 ggcgacttgg gtggaaacga taaaacgcga agtgttggcc gcattacgcg atgaggaagg
9421 gtggcgtcca tgagtctgat tgaacgtggt ttagctaagc tgacaattaa tgcttataag
9481 gatagggaag ggaagatacg ggcaggaacg ttgcaggcca tgtataaccc tgactccttg
9541 caactggatt accaaacgga ttatcagcaa tcccaagcga ttaatagcga aaagcaaagt
9601 agcatttatg tacaggccaa gcccgcaggg ttatcacttg aattaatttt tgatgccacg
9661 atgccgggta acaaaacccc cattgaagag cagctcatgc agctcaagca actgtgcagt
9721 gtggatgcaa ccagtaacga gacgcgattc ctgcaagtta aatggggcaa aatgcgttgg
9781 gaaagtcggg gttactttgc tggcagggcc aagagtttgt ctgtgaatta cactttgttt
9841 gatcgtgatg cgactccctt gagggtacgg gtaatattgg cattagtggc tgatgaaagt
9901 ctggtgttgc aggagactga acaaaatctg caatctccgg caaaaatcgc attacgcata
9961 caggatgggg tatctctggc tctgatggca gccagtacgg catcaacatt gtcaggcggt
10021 gtggattatc tgacgctggc ctggcaaaac ggtctggata atctcaatgg gttcgttccg
10081 ggtgaaatat tgcaggccac caggggagac gaatcatgag ccaccaactg aaaattattg
10141 cagatggtaa ggcactgtca cttttggccg cggtagatgt ggacacctgt tatcgggtta
10201 acagtatacc ttctgcgaca ttgaaactga gcgtaccgga taggccactc tcttctttca
10261 gtcagacgga tgttcagaca gaactggccc actgtcaggt agggaaaacc ctgcgtctgg
10321 aattgattga tggtagcaaa aaatgggtgc tgtttaatgg tcttattacc cgtaaggctc
10381 tgagaattaa gaataagcaa ttattgctca ctctggttgt caagcatcgg ttgcaactga
10441 tggtggatac ccagcattca cagctgttta aagacaaaag cgaaaaagcg atcttaagca
10501 cgctattgaa tcagaccgga atcaatgctc gcttcggaaa gatagcggcg ttagatcaaa
10561 agcatgaaca gatggtgcaa tttcgttgtt cagactggca ttttctgttg tgccgactgt
10621 cggcaaccgg tgcatggttg ttacctgcca tagaagacgt tcagtttgtt caacctgatg
10681 ctctgaaatc aaactcagcc tataccttga agagcagggg ggatgagaac aaagacatcg
10741 ttgtcaagga tgcttactgg cagtttgaca atcaaatcaa ccccgctttg ctggaagtca
10801 gtggctggga tatcagtaag cagcaggtac aatcaggcgg tcgctacgga aaaatcgcgt
10861 tgggtaaggc ggcactctct cctgatggat tggcatccct taataaaacg ggttgggaca
10921 tttgttatag cagtccgtta acaacccagg aaagcggtta tctggcacag ggattattgc
10981 ttaaccagcg catttctggg gtgacaggag aatttttgct caaaggagat gggcgttacc
11041 agttgggaga caacattcag ctgactggat ttggttcaca gttagatggt acggcaagca
11101 ttactgaggt tcgccaccgt cttaatcggc gaattgattg ggaaaccacg gtgagcattg
11161 gtttacaaca tgaatatttg ccgatattac ctgatgctcc cgaactacat attgcgacag
11221 tagcgaaata tcagcaggac agtgcggtgt taaaccgtat ccccattatt ctgccggtac
11281 tgaatcgtcc caatgaattt ttgtgggcca gattggggaa accttatgct agccatgaaa
11341 gcggtttctg tttttaccca gagccaggtg acgaagttat tattggtttt tttgaaaatg
11401 atccgcgtta tccagttatt ttaggtgcta tgcataatcc gaaaaataag gccccttttg
11461 aaccaaccca agataatagg gaaaaagtat tgatcgttaa aaaaggtgaa gcgcaacaac
11521 aattagtcat tgatggcaaa gagaaaatga tccgaattaa tgcgggtgaa aatcaaataa
11581 tgcttcagca agataaagac atttctctgt caacgaaaaa agaattaaca ctgaaagcgc
11641 agacaatgaa tgccacgatg gataaatcat tggcaatgtc cgggaaaaac agtgttgaaa
11701 tcaaaggcgc aaaaattaat cttacccaat gaaaggtgac gatgaatgga aaatcaaata
11761 ctgacacaac tctatggtcg tggttgggct tttcctccgg tcttttccct tgaaaagggg
11821 gtagagatgg ctgaaggggc ggaagatgtg agacaaagtt tgcagattct gtttagtact
11881 gagccggggg aacgtcttat gcgtgaaaat tatggctgcg gattaaatga ttttatgttt
11941 gaaaatatcc gcaatgaact tattgctgaa attgaatccc atatccatga caacgtatta
12001 cgatatgaac cccgggctga tatgactgat attcaggttc gtcaatcccc tggcatgggg
12061 aatactttgc aagtgcaggt catgtatcgc ctgagaggga gtgatatcaa tcaacaaatc
12121 cagggagtac ttgcactgag tgaaggccgg gtgacggagg tagtatgagt gaagcgattg
12181 tggtggatgg tgacgtgtta cagtttgatc ccaactttgg caatcggcag gtgacggttc
12241 ccagcccagg aaaaattagc ggcacaggac atgcgcaggt aagtggaaaa aaagtgtgta
12301 ttctggggga tgagaaacag gtcagggttt ctgcaaccta tattacaaca acacatacta
12361 cgccgggaac aggaaccatt actatcagtg ctctggatgc tggccagcag gcccttcagt
12421 gtaccagtgg ggcggcttta attatcaagg ggcagcaatt tacggcgatg tttacgcctg
12481 aattgccagc catgaataat acagtgactc cgccacaacc ggatgttacg acaccttcat
12541 caggaaaagg acgttttatc actcaacaaa attttgctac cgtaaattag agtattgact
12601 gaattaaata gaattaacga aggtgtaaat aattatttat ttgctgacga atcgctgtga
12661 caaataaaca caggtgatgt tatggaatta aatgagttaa ctaacaaatt gtcaaatttg
12721 gtgccaatga ccgattttaa attagataat cgagccagtt tgcaattgct taaatatatt
12781 gaagcgtata cgaagataat accctttaat tctggcgata aatattggaa tgactttttc
12841 tttatgtcag gaaatacgcc agagaaactt gcaaaattat atcagaaaga aatagaaccc
12901 aatggggagt tattacctca gcaggctttt ttgttggcgg ttttgcgttt attggaaaca
12961 ccaatatcct tattaaatgt attacctgct gctcatcgtg agctctatta tcgggagctt
13021 ttaggcttgt cttcccatgc ggcacagcct gatcaggttg ctttatctat ggaactgaat
13081 tcgacagtga tggaacagct gctccctgaa ggaaccctgt ttgaggctgg tcaggatgaa
13141 caaggcaatg cattgcaata tgccctggat gccagtttgc tggctaatcg tggatatatc
13201 agtgacttgc gctggttacg gaatgacggg gaaaagcaat gggttacttc tgctccatgg
13261 gatttacagg cacaggtgtc actgccgtct gatgggatac gattatttgg taagacaaat
13321 agtgatcagc aggtatttgg tggggtgttg ataacgtcat cacttctggc gatggaagcg
13381 gggataagga agatcattgt tacttttgag caggagatga acacccaaga actggtggca
13441 caggtcagca gtggaaatca atggctaaca ttgacgtctg aggtaaataa gaaagaggtc
13501 acactgacac tgtcagacaa agaaccggca atcagtgcgc cagaggatct ggataatctc
13561 tttttcacgc aaccggtact caggctacag ggaaaggata gtcaggcact gccggaggtg
13621 acgggtatca gcgtttcgga aaaggatgat actaaggata cctcttttga gatgtatcac
13681 ttaacaccat ttggttatag cagtgatata gagccattgg aggaaaatcc agcgttatat
13741 ttaggcttta ctgatgtaaa gccagggcaa acactggcgc tgtattggaa attaaaatcc
13801 ccgcagcaac caaccgtttc ctggtattac ctggatcaac ataatcaatg ggctgaattg
13861 gattcatggg tcagtgatgg aacccagaat ctgtatcagg atggtacttg gcacgttgag
13921 ttgcctgtgg atgcatccaa tcaggcagag cagatgccag ttggacgcta ttggttgcgg
13981 gcagtggtgg aggtacccgc tcatgagggg gcgttgggga aggctccttg gctatatggt
14041 ctaatctata acgccatgac ggcaaccttg gttaatgtag atagcatcag tgacagccat
14101 ttcttaaccc ctttgcctgc cagcagcata cagcggcccg ttgaacccat cattgtgttg
14161 gcatcggtca accagccttg ggcatcatgg ggtggacgta tacctgaatc ctacagtgcc
14221 ttttttgaac ggatagctca aaacctgtct catcgaaacc ggtccttaac ctggggaaat
14281 atggtgacat tactcaaaga gcgttatgtc agcatctttg atgttaagta tccaggtaat
14341 gatgaactca ccagagtgcc agcattggag cagcagcaac taacagtgat tccagcaaac
14401 cggtacaacg atagcgatga ttctctgcgt ccggtactga atcctgctcg tctgcaagag
14461 atggctgatt ggttgcagca gaaagactct ccctgggcct ctattgaggt caggaatcca
14521 gaatacttgg atgtgaaaat ccattacgag gtgattttta aacctgatgt gaacgaagat
14581 tttggctatc gccagctaca gcagcaactg tgtgaggtgt atatgccttg gagcatagat
14641 gagcagcggc ccgttgtatt gaataacagc attaattatt tccagttgtt agccactatt
14701 caacagcaac cgctggttga gcgagtcact cgtctgacac tacatcgggc tgattcttct
14761 gatgagagtg atggtacagc atctgtggaa gccaaagata atgaagtgct tattttagtc
14821 tgggaagagg acgataatct gcaataccga ggaaatgact atgagtaatc aggatgcact
14881 gtttcatagc gttaaagacg atattcactt tgataccttg ctggaacaag ctcatcaggt
14941 gattgaaaaa caggctgaaa aactgtggag tgatacggca gagcatgatc cgggtatcac
15001 atttttgcag ggaatcagtt acggtgtgtc agatttggct taccgacata cattacccct
15061 gaaagattta ctgactccgg cgccggatga gcagcagcaa gagggaattt ttcctgccga
15121 atttggcccg cataatacac tgacttgtgg gccggtgaca gcggatgatt atcgcaaggc
15181 attgttagat ctacacagca gcgacagcct ggatggtact cagcaggatg agggggattt
15241 tctgttccgg agtgtgcaac tggtgcgtga accggaaaaa cagcgttata cctattggta
15301 tgatgcaacc aagagggaat atagctttgt caacagtgaa ggggctaaag agtttacctt
15361 gcgggggaat tactggttgt atctggaacc aacccgttgg actcagggta atattgccgc
15421 tgctaccaga caactgacag aatttttgac taaaaatcgc aatattggtg aatctgtcag
15481 caacattatc tggctacaac cggttgatct gccactgttg ctggatgttg aactggatga
15541 tgatgtaggt gcacaggatg tccccggtat ttttgcggcg gtgtatagca ccgcagagca
15601 gtatctgatg cctggagcac agcgttaccg tacggaagta ctgcaaaatg ctgggatgag
15661 caatgatcaa atcttcgaag gtccattatt ggaacatggc tggataccag agctgccggc
15721 agcccgtgat tatactcaaa ggctcactct caatcttagc cggttggtaa atagtctgct
15781 tgagattgag ggcattaaac atgtgaatcg tcttcgtctg gatgatagct tcgataaaac
15841 tgctattgaa cccgttaagg gggatacctg gtcgtggtcg atcaaagagg gctattatcc
15901 acgtctttgg ggagaagacc cacttaacca attggcgcaa caaaatggcc cgcttagggt
15961 gatagccaaa ggagggatta gcgtcagtgt gagtaaagag caaatccagg ccagtttacc
16021 cagtcaatca ctgattcaaa atgagccggt aatattggct tacggccagc accgtgacgt
16081 tggcagctat tatcccgtca gtgatacttt gccgccttgc tatggactac aacattcttt
16141 gtctgaaagt gaacacttat tgccacttca tcaatttatg ttgccatttg aacaattatt
16201 ggcctgtggt tgtcaacaga tagccatgct cccgcggtta ctggcttttc agcgcgaagg
16261 ttatgaggtt tggggtgatc agtggccctt taagtcaggc tcagtgaatg atgacgccca
16321 tcaagattat gcccctgcat taaaggattt gttaggacag attgcgctgg atagtgatca
16381 tgaattggat attattaatt acttgctggg ttactttggc acacagcggg caccgcgtac
16441 ctttacgaca caactcgatg attttcgtgc ggtccaacag ggttatctgg cccagcaacc
16501 gacattgact taccaccgct ccaatattcg tatcgatcag gtatcgtcgc tacaaaaacg
16561 tattgctgct cgcatggggc tgggcggtga gttgtttaaa cctcaaccgg atctgagcca
16621 actgcctttt tatttgattg aacatcgagc gttgctgcca gtcaaaccca atagtcagtt
16681 tgataaggaa cagaaaccag cctcggtgac agaggagggg ggcagccaaa caggtcaaca
16741 ttatgtggtc attgaacaga agggcattga tggcaagctg acacaggggc aagtgatcaa
16801 tttaattctg tatgaaggag agcagggaga aacccaattt acgatacgcg gtcagatggt
16861 attcaaaacc gagggggata agttttggtt ggatgtgaat aatagtgcgc aactggaata
16921 taatctggcg cgggtaatga cagcagccaa ggcgagtaaa ctcttttggc aaaacagccc
16981 ggtatggatg gaggatatgg gctatcgtct ggcctatgct agtgaccaat cctcattgcc
17041 tgtgaatcaa cggcgcttga cccgcacagt gcaaactcca ttcccgccga tggttgttgt
17101 aggtagcgaa atcaccctgt taaagcaggt ggggatagtc aatttaaaaa aagcggagtc
17161 agaaaaactt tatgcaaaag ttgttagctt tgatcgcatt gaagggacct tgattattga
17221 gcgtttgggt aattccactc tggcttttcc tacctcggaa gaggcgtggc ggtatagttg
17281 gtatttttcg ggggagaaat atgaaaggac tgaccgcttt tcatttgtga ttagcgtagt
17341 agtgaacagt gacttaatta aattgcccgg tgttgatccc tataaattgg aagaatgggt
17401 gaaagaaacg attcttaccg aatttccagc tcatatttct atgattatcc attggatgga
17461 tcgggaagcc tttttaaatt tcgccaatac ctatcagcgt tggcaaaata atggtacgcc
17521 actgggggat gcggcttatt ccattctaga aagtttgaca cttggtaaat tgccatctgc
17581 cttaaaaggt gttggcacaa tgcgtattgc cacatctagt caaagagaag aagtggtggg
17641 tagtaatggt gatcaatgga atacagatgg aataacccag aatgaattat tctatgttcc
17701 taaagagagc taggaaaaat aaatatctgc cactaatgat gttgaattaa atatgttttc
17761 tggagttaat catgaacgaa actcgttata atgcaactgt acaagaacaa caaacattat
17821 ctaatccaaa agctgttgga cctgacatcg ataaattaaa ggataaattt aaagagggca
17881 gtattcccct gcaaaccgat ttcaatgagt taattgatat tgccgatatt ggacgtaaag
17941 cctgtggtca agcgccacaa caaaatggcc caggagaagg attgaaattg gctgatgacg
18001 gtacgcttaa tttaaaaata ggcacttttt ccaataaaga cttttctcca ttaatattaa
18061 aagatgatgt tttatctgta gatcttggta gtggtctgac taatgaaacc aatggaatct
18121 gtgtcggtca gggcgatggt attacagtta acactagcaa tgtagctgta aaacaaggta
18181 acggaattag cgttactagt agtggtggtg ttgccgttaa agttagtgct aataagggac
18241 ttagcgttga tagtagtggt gttgcagtta aagttaatac tgataaggga attagcgttg
18301 atggtaatgg tgttgcagtt aaagttaata ctagtaaagg aattagcgtt gataatacag
18361 gtgttgcagt tatagctaat gctagtaagg gaattagcgt tgatggtagt ggtgttgcag
18421 ttatagctaa tactagtaaa ggaattagcg ttgatggtag tggtgttgca gttatagcta
18481 atactagtaa aggaattagc gttgataata caggtgttgc agttatagct aatgctagta
18541 agggaattag cgttgatggt agtggtgttg cagttatagc taatactagt aaaggaatta
18601 gcgttgatgg tagtggtgtt gcagttatag ctaatactag taaaggaatt agcgttgata
18661 gtagtggtgt tgcagttaaa gttaaagcta atggcggaat taaagtagat gctaatggtg
18721 ttgcaattga tcctaataat gtactcccca agggagtgat tgtaatgttc tctggcagta
18781 ctgcaccaac tggttgggcg ttatgtgatg gcaataatgg tacaccaaat ttaatcgatc
18841 gatttatttt aggtgggaaa gggactgata ttaatggagt gagtactaat acagcttcag
18901 gtactaaaaa tagtaagtta ttcgatttca gttctgatga agctacatta actattgatg
18961 gtaaaacact ggggagaggt ggctctggtg gtggcggttc tggcggccac gatcatgata
19021 ttaagataac gggcacagga aaacattctc acaaaaacaa agtaacagtc ccttattata
19081 ttctggcttt catcataaag ctttaatata tatgaaaaat tgaaaatata aattatccat
19141 taataataaa gaggatatta gcatgacttc ggagccaaat ctgttaaacc ggattacaat
19201 tactattgaa gctaataatc aacaagtagc tagaaaagta ttgcatggct ccttgcttaa
19261 tcaagctaat ataaataaat tatttaattc atactttaat gaatatgaaa ttaatagggg
19321 tgtttattta gaaacattaa tcctgaatct tggtacgata aatttccatg attttaattc
19381 attgtttcct actctcctaa aagctgcatt gaataaagaa ttcagtcaat atcagataaa
19441 caaccatagg gaagaaatgc tatttaatga gacaatatca aatcaagcta ctgataagtc
19501 ttacatattt ggcgataaca aattaattga tgcagagaat ttcattcact ttttatatca
19561 aaagcattcc acattaaatc tagtagaagc aatgggaaat aatggtattg aaaaattaac
19621 aaatcagtta acacaaatag aaaataaatt tgcgttatta ttggcaaaaa gttgtttgtc
19681 tgaggaaggc ttaaaacgac tcttggctat caaacaaccc gatttattaa tcgctatcaa
19741 tcgcagatta tctgaaagaa taaatagacc acaatatcag gagaagcttg tttcctgcgg
19801 acaactgata tttagtgctc tgggatatat acaacagtac aatatacagg aaattcctaa
19861 accggatgaa aaagttattg cacgcataac aactgaactt aataataatg gtttgcttaa
19921 tacaatacct attattacac tatttcgtca gagtgggatt aacgattcat cactaaatga
19981 ttggctaaag aaaatctggc aggtgagatc aatttcacag ttatgcagaa agtatctttc
20041 tgctaaggaa taccaatatc tgtcagaaca ttttgtttca aagagcgtcg ataaaaatag
20101 atatgatgaa gagcccgtaa atcagagcat attatcaagg ttgaataata attccattaa
20161 agaaggaaat aatcacagtc aactctgtac tctcagtaga ctatattctg aacccgttgt
20221 attacctgaa caaaccattc tacgtcaggt tagtaataca gtagatcaga gcatattatc
20281 aaggttgaat aatgcctcca ttaaagaagg aaataaccaa agtcaacttc gcactctcag
20341 tagactatat tctgagcccg ttgcattacc tgaacaaacc attccacgtc aggttagtaa
20401 tacaggtata ttaattctat ggccaatgct acctacacta tttaaccagc ttggtctact
20461 tgagaaaaag aaatttatcc atcgtcaggc ccagtttaat gccgttgatt ttcttgatta
20521 cctgatttgg ggaaccgaag atgtgaaagt ggaacgaaag gttttgaata atgttctatg
20581 tgggttaatg gctgatgaaa ttactgaacc aatgcctatt gaaccagaaa aacaatggat
20641 aataattcaa tggctggacg ctattatctc ccaactttct ggctggaaaa agttaagtcg
20701 taatgacgtc cgtcaattat ttctacaacg accaggagaa ttactgatca atgaacagga
20761 aattaaaatc acaatacagc aacaaccatt tgatgctctg ttaactgatt ggccgtggcc
20821 aatgaatatg gcttgtttta gctggttgag tcaaccatta accattacgt ggttataacc
20881 attgaccaca atgacttagt ctgagtaaaa aatatgaata tatcgcctgt tttttatgat
20941 tcattgaatc aggataacga ccgtgatcta tcgtttttat ttagcgaact ggaacgaata
21001 gatctcgctc ttcaacacca tttttattgt gtagaaagtc agcgaagtga gctcctggat
21061 gagtttctgc tcactgaggc ggaagtggtg accaggctgg ataagccact tggtaaacct
21121 cattggataa atgatgatta tctggcgata tcgcaaaagg gcaatgtaag cctaatggca
21181 gcgtccagat taatggatct gatcgaacgc tttgaactga ctgattttga gcgcgatgtt
21241 ttactattag gcttattgcc ccattttgat agccgctatt atcgactgtt ttcgctgatt
21301 caagggggac aacagggtcg attaccttct tttgcgctgg cattggaact gttttgccac
21361 tcggcgctgg agaaacaggt acagcaagcg agttttctgc accgggcacc tttgatgggt
21421 tgccagctat tatccatcga tactagtcaa aaaacgctgg cctggctcca gactcccttt
21481 attactgaca gcggggtata tcacttttta ctggggcatc actacattat gccggcttta
21541 gaacattgtg ctgagtggtt aacaccgaca gggattggct gttatcctga aggattaaaa
21601 caagtactgg gtaacgtatt gttatctgac aacgataata ttagaccgat tgtcttatta
21661 cggggaatgg ccggcagtgc cagagcttat accattacta atatgatggc ttcagaaggg
21721 aagcaaacac tgctggtaga tatatccaaa cttgctgata gcgatgaaaa aaacattatt
21781 cttcagataa agcatatttt gcgggaaacc cgcatgcatg gagcatgttt attattacgg
21841 aatttttgct tgttagtgga acagaataaa caactattgg actccctgtc agagttattg
21901 aatcaacctg aattaagaat tgtttgcctg attgagcctt attccccatt ggtatggctg
21961 aaaaagatac cggtattact gattgagatg ccacttttaa cgcctgcgga aaaagccaga
22021 ttgttaattg ccagcttacc ggataattgt tccgaggata ttgatacgat aactttaagc
22081 cagcgttaca cttttaaccc agaaaccctg ccattgattt tgcaagaggc ccagctttat
22141 caacagcagc gagatccgct ggatatcttg cagcaatgcg atatacgcca ggcattaaat
22201 ttgcgtgctc aacaaaattt cggtcaattg gcacagcgga ttattcctaa gcgctcatta
22261 aaggatttat tggtatccga tgagattgct cagcagttac gggaaatact catagcaatt
22321 aagtatcggg aacaggttct ggcgggaggg tttaaagata aaattgccta tggcactggt
22381 atcagcgccc tgttttatgg tgattcaggc actggaaaaa ccatggcagc agaagtgatt
22441 gctgaccaca ttggcgttga cttaataaaa gtggatttat ctacagtagt gaataaatac
22501 atcggtgaaa cagaaaaaaa cttatcccgt attttcgatt tggcggaaca ggatgcaggg
22561 gtattattct ttgatgaagc tgacgcactg tttggtaaac gcagtgaaac taaagattcc
22621 caggacagac atgccaatat tgaagtttct tacttattac agcgcctgga gaattacccg
22681 ggtctggtca ttttatccac caataatcgt ggtcatttag acagtgcttt taatcgtcgt
22741 tttactttca ttacccgttt tacttacccg gatgaaaaaa tccgtaaaaa aatgtggcag
22801 gaaatttggc ctagaaatat aaaaatatcg gaagatatcg attttaacga attagctcaa
22861 cgaacaagcg tgactggcgc gaatatccgc aatattgctt tattgtcttc attctttgct
22921 tcagagcagg ggaatgatga agtcagtaat gaaaatattg aaattgcatt gaagcgtgaa
22981 ttagctaaag tcggacgatt aacattttaa aagttatcac aatgaaagta ttgaaatatt
23041 aaataaattt attaccaaaa agttatcacg atataattta agagaggttt tttatgttaa
23101 acacgcaaac tattattgat gtcaataagg caatggatgc catgctgcgc gcatatctga
23161 atcaagatat tgccattcgt tttgatctac ctgaattgga tactatgcaa tctgatgcga
23221 tggtaagtat ctttctttat gacattcatg aagatttaca gcttcgctcg gcagaatcaa
23281 gagggtttga tgtttatgcc gggaggttat tgcctggttg ggtaaatatt aaatgtaact
23341 atctgattac ctattgggaa gcttctaagc cagcgactga tgccagcagt ccggatagcc
23401 aacctgataa ccaggcaata caagtgatgt cacaagtatt aaatgccttg attaataatc
23461 gtcaattggc aggtattcct ggtgcttata ctcaggttgt accgcctaaa gagagtttaa
23521 atagcctggg gaatttctgg caatcactgg gtaatcgccc acggctttct ctcaattatt
23581 cagtgacagt acctgttagc ctaaacgatg gtcaggatag cgcgactccg gttaccgcgg
23641 tttcttctac agtggaacaa acggcatcgc tcagtcaaga agtggttagt catgctttac
23701 gcgaattact cattacggaa ttaggaggag gagaggataa ccggttggta ctgagtaaag
23761 ttgaattatc cgcagtgaaa gagacgatga ctcaagacag tccggctcag atgattatat
23821 tgttgtctgt ttcaggcatt acacgacagg aatatttgaa ggaaattgat aatatctttg
23881 atcgttgggt aaataatgct gaagttatta ccactattga tgattgtggg attagaattg
23941 aaagtataac gaaagataat cttgtaggaa tttaaatttt tttaattaaa taaaggaatt
24001 aatattgatt tgatagagca tgtaatcaat aacttctatg aaaatgttga aaaacaatac
24061 acggaaacac ttaacttaca attaaattgt taaaggagat gatgataaga atggtcagtc
24121 accagataaa atgatctctg gtcaatttta ggaaagctga aaaaacctga ccaaatgcgt
24181 cgtaaattct atgcttagaa agactcggtg ccagtgtaat acctaagata tggtttttat
24241 ttccatatct gttcgaaaac ttagaaataa caagttcaac aataaccagg gactttaaat
24301 atcggatcct tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt
24361 attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat
24421 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
24481 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
24541 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
24601 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
24661 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
24721 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
24781 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
24841 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
24901 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
24961 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
25021 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
25081 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
//
