| 出品公司: | |
|---|---|
| 载体名称: | pG5luc |
| 质粒类型: | 哺乳动物双杂交系统 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 4955 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pG5luc 4955 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4955)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4955
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 219..228
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 225..1877
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 1918..2039
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2458..3046)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3217..4077)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4078..4182)
/gene="bla"
/label=AmpR promoter
rep_origin 4209..4664
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 4795..4843
/note="synthetic polyadenylation signal"
misc_feature 4857..4948
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggtaccgagt ttctagacgg agtactgtcc tccgagcgga gtactgtcct ccgactcgag
61 cggagtactg tcctccgatc ggagtactgt cctccgcgaa ttccggagta ctgtcctccg
121 aagacgctag cggggggcta taaaaggggg tgggggcgtt cgtcctcact ctagatctgc
181 gatctaagta agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa
241 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
301 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
361 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
421 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
481 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
541 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
601 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
661 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
721 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct
781 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta
841 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta
901 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
961 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
1021 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc
1081 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt
1141 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag
1201 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta
1261 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
1321 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
1381 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg
1441 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
1501 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag
1561 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg
1621 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
1681 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
1741 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
1801 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
1861 gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc gcttcgagca gacatgataa
1921 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
1981 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
2041 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
2101 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc
2161 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
2221 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc
2281 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
2341 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
2401 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
2461 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
2521 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
2581 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
2641 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
2701 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
2761 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
2821 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
2881 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
2941 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
3001 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
3061 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
3121 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
3181 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
3241 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
3301 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
3361 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
3421 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
3481 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
3541 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
3601 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
3661 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
3721 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
3781 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
3841 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
3901 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
3961 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
4021 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
4081 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
4141 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
4201 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
4261 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
4321 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
4381 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
4441 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
4501 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
4561 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
4621 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt cgccattcag
4681 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcccaa
4741 gctaccatga taagtaagta atattaaggt acgggaggta cttggagcgg ccgcaataaa
4801 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca
4861 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt
4921 gcaagtgcag gtgccagaac atttctctat cgata
//
