| 质粒名称: | FUmGW |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22479 |
| 存储实验室: | Connie Cepko |
|
载体骨架基本信息 |
|
| 载体名称: | FUGW |
| 载体出品公司: | I. Verma, Salk |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 9165 bp |
| 完整质粒大小: | 9964 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | membrane-bound green fluorescent protein |
| 物种来源: | Aequorea victoria |
| 插入大小: | 799 bp |
| 基因又名: | mGFP；GFP |
| 标签/融合蛋白: | palmitoylation (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI, BamHI, AgeI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | N/A |
|
文献引用方法 |
|
| 材料和方法部分: | FUmGW was a gift from Connie Cepko (爱迪基因 plasmid # 22479) |
| 参考文献部分: | Retinal progenitor cells can produce restricted subsets of horizontal cells. Rompani SB, Cepko CL. Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):192-7. 10.1073/pnas.0709979104 PubMed 18162542 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FUmGW 9999 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FUmGW
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9999)
AUTHORS Rompani SB, Cepko CL
TITLE Retinal progenitor cells can produce restricted subsets of
horizontal cells.
JOURNAL Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):192-7.
PUBMED 18162542
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|FUmGW|
FEATURES Location/Qualifiers
source 1..9999
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind 3658..3677
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 3933..4652
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3978..3999)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4239..4258)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4586..4607
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 4691..5279
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4744..4764)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5162..5173)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5282..5298)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5283..5299)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5804..5984
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6010..6027)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6016..6240
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6286..6714
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6373..6392)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6583..6604
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6723..6743)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6728..7057
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6908..7043
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6970..6989
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7105..7152
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7171..7545
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 7675..7796
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7712..7731)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7766..7785
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7845..7861)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7845..7861)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7858..7880)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7869..7885
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7893..7923)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7938..7959
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8076..8093)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8247..8835)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8327..8346)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9006..9866)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9629..9648
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9867..9971)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg
2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc
2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaag
3781 cttctgcagg tcgactctag aaaattgtcc gctaaattct ggccgttttt ggcttttttg
3841 ttagacagga tccccgggta ccggtcgcca ccatgctgtg ctgtatgaga agaaccaaac
3901 aggttgaaaa gaatgatgag gaccaaaaga tcatggtgag caagggcgag gagctgttca
3961 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
4021 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
4081 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
4141 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
4201 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
4261 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
4321 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
4381 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
4441 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
4501 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
4561 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
4621 tcactctcgg catggacgag ctgtacaagt aaagcggccc ggaattatcg ccgatatcaa
4681 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
4741 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
4801 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
4861 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
4921 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
4981 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
5041 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
5101 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
5161 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
5221 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
5281 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
5341 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
5401 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
5461 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
5521 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
5581 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
5641 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
5701 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
5761 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
5821 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
5881 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
5941 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
6001 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
6061 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
6121 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
6181 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
6241 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
6301 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
6361 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
6421 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
6481 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
6541 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
6601 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
6661 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
6721 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
6781 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
6841 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
6901 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
6961 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
7021 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
7081 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
7141 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
7201 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
7261 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
7321 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
7381 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
7441 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
7501 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
7561 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
7621 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
7681 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
7741 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
7801 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
7861 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
7921 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
7981 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
8041 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
8101 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
8161 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
8221 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
8281 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
8341 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
8401 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
8461 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
8521 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
8581 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
8641 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
8701 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
8761 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
8821 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
8881 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
8941 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
9001 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
9061 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
9121 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
9181 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
9241 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
9301 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
9361 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
9421 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
9481 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
9541 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
9601 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
9661 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
9721 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
9781 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
9841 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
9901 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
9961 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
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