| 出品人: | Bob Weinberg |
|---|---|
| 载体名称: | pBABE-hygro ; pBABE hygro |
| 质粒类型: | 逆病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | MSCV |
| 载体大小: | 5578 bp |
| 5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
| 3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Hygromycin |
| 克隆菌株: | DH5α、HB101等 |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: |
推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。 |
| 产品目录号: | #1765 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBABE-hygro 5578 bp DNA SYN
DEFINITION pBABE-hygro
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5578
/organism="pBABE-hygro"
/mol_type="other DNA"
promoter complement(4..32)
/label="AmpR_promoter"
misc_feature 102..569
/label="5_LTR2"
misc_feature 108..569
/label="3MoMuLV_LTR"
misc_feature 639..1440
/label="psi_plus_pack"
misc_feature 1408..1424
/label="pBABE_5_primer"
misc_feature complement(1498..1518)
/label="pBABE_3_primer"
misc_feature complement(1504..1883)
/label="SV40_enhancer"
promoter 1516..1784
/label="SV40_promoter"
rep_origin 1683..1760
/label="SV40_origin"
CDS complement(1744..2664)
/label="ORF frame 2"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAKFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPPGIRSAGLPNLTRGRPSWQ
AFCKSLGLQKSLLTTSGIAQRPRRPRPLHK*"
misc_feature 1745..1764
/label="SV40pro_F_primer"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAKFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPPGIRSAGLPNLTRGRPSWQ
AFCKSLGLQKSLLTTSGIAQRPRRPRPLHK*"
CDS 1906..2931
/label="ORF frame 1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
gene 1921..2928
/label="hygroB"
/gene="hygroB"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
gene complement(4310..5540)
/label="Ampicillin (w/ gaps)"
/gene="Ampicillin (w/ gaps)"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
CDS complement(4680..5540)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
61 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
181 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
241 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
301 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
361 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
421 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
481 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
541 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
601 cccagggacc accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt
661 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
961 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
1021 tagggccaga ctgttaccac tcccttaagt ttgaccttag atcactggaa agatgtcgag
1081 cggctcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
1141 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
1201 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
1261 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
1321 gtacacccta agcctccgcc tcctcttctt ccatccgcgc cgtctctccc ccttgaacct
1381 cctctttcga ccccgcctca atcctccctt tatccagccc tcactccttc tctaggcgcc
1441 ggccggatcc cagtgtggtg gtacgtagga attcgccagc acagtggtcg accctgtgga
1501 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
1561 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
1621 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
1681 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
1741 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
1801 gaggcttttt tggaggccta ggcttttgca aaaagcttgc cagctggggc gccctctggt
1861 aaggttggga agccctgcag atctgatccc ggggggcaat gagatatgaa aaagcctgaa
1921 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
1981 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
2041 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
2101 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga atttagcgag
2161 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
2221 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
2281 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
2341 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
2401 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
2461 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
2521 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
2581 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
2641 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
2701 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
2761 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
2821 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
2881 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gagtagatgc
2941 cgaccgggat ctatcgatgc ggcgggtgtg gtggttacgc gtaatctgct gcttgcaaac
3001 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
3061 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
3121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
3181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
3241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
3301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
3361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
3421 aggagagcgc acgagggagc ttccagggga aacgcctggt atcttatagt cctgtcgggt
3481 ttcgccacct ctgacttgag cgtcgatttt gtgatgctcg tcaggggggc ggagcctatg
3541 gaaaaacgcc agcaacgcat cgataaaata aaagatttta tttagtctcc agaaaaaggg
3601 gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa
3661 ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg
3721 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
3781 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
3841 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
3901 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
3961 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
4021 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
4081 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
4141 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacatgc
4201 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
4261 gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
4321 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
4381 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc ccaaggcggt
4441 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
4501 gcaaaaggcc aggaaccgta aaaaggccgc gttacgctca gtggaacgaa aactcacgtt
4561 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
4621 aatgaagttt gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
4681 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
4741 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
4801 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
4861 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
4921 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
4981 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
5041 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
5101 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
5161 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
5221 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
5281 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
5341 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
5401 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
5461 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
5521 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagg
//
