| 质粒名称: | pLenti-DsRed_IRES_MAPT:EGFP |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 92196 |
| 存储实验室: | Huda Zoghbi |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti-GPS-GAW-IRES |
| 空载体大小: | 8000 bp |
| 完整质粒大小: | 11415 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | DsRed-IRES-MAPT-EGFP |
| 物种来源: | H. sapiens (human), Synthetic |
| 插入大小: | 3368 bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | ClaI (未破坏) |
| 3’克隆位点: | XhoI (未破坏) |
| 5’测序引物 | IRES-F: GCCCCCCGAACCACGGGGAC |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti-DsRed_IRES_MAPT:EGFP was a gift from Huda Zoghbi (Addgene plasmid # 92196 ; http://n2t.net/addgene:92196 ; RRID:Addgene_92196) |
| 参考文献部分: | TRIM28 regulates the nuclear accumulation and toxicity of both alpha-synuclein and tau. Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L, Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY. Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809. 10.7554/eLife.19809 PubMed 27779468 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLenti-DsRed_IRES_MAPT:EGFP 11269 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral overexpression vector to make stable bicistronic cell
line for Tau screen.
ACCESSION .
VERSION .
KEYWORDS pLenti-DsRed_IRES_MAPT:EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11269)
AUTHORS Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J,
Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L,
Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
TITLE TRIM28 regulates the nuclear accumulation and toxicity of both
alpha-synuclein and tau.
JOURNAL Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
PUBMED 27779468
REFERENCE 2 (bases 1 to 11269)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11269
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 3..636
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 683..808
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1305..1538
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1723..1767
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2060..2177
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2229..2532
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2533..2736
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2687..2707
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2733..2757
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2840..2849
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2846..3523
/codon_start=1
/product="rapidly maturing tetrameric variant of DsRed
fluorescent protein (Bevis and Glick, 2002)"
/label=DsRed-Express
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
LFL"
primer_bind complement(2976..2994)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind complement(3026..3046)
/label=DsRed1-N
/note="DsRed1, reverse primer"
primer_bind 3244..3263
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 3438..3461
/label=DsRed1-C
/note="DsRed1, forward primer"
misc_feature 3549..4122
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 3572..4124
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3716..3733)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3943..3962
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5477..6196
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 5477..6193
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5522..5543)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5783..5802)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6130..6151
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(6216..6235)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6217..6235)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6280..6303)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 6665..6684
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 6753..7547
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6807..6826)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7417..7436
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
misc_feature 7661..8249
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7714..7734)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8132..8143)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8324..8557
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(8676..8694)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8762..8866
/gene="bla"
/label=AmpR promoter
CDS 8867..9727
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9085..9104)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9898..10486
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10387..10406
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 10640..10657
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 10749..10768
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 10897..10918
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 10933..10963
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10971..10987
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 10976..10998
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 10995..11011
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 10995..11011
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 11061..11080
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
61 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
121 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
181 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
241 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
301 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
361 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
421 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
481 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
541 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
601 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
661 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
721 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
781 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
841 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
901 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
961 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
1021 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
1081 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
1141 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
1201 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
1261 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
1321 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
1381 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
1441 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
1501 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
1561 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
1621 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
1681 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
1741 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
1801 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
1861 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
1921 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
1981 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa
2041 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
2101 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
2161 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggactagtg
2221 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
2281 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
2341 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
2401 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
2461 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
2521 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
2581 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
2641 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
2701 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
2761 tggagacgcc atccacgctg ttttgacctc catagaagac accggcggcc tagcgctacc
2821 ggactcagat ccaccggtcg ccaccatggc ctcctccgag gacgtcatca aggagttcat
2881 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
2941 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
3001 ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta
3061 cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa
3121 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
3181 cctgcaggac ggctccttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga
3241 cggccccgta atgcagaaga agactatggg ctgggaggcc tccaccgagc gcctgtaccc
3301 ccgcgacggc gtgctgaagg gcgagatcca caaggccctg aagctgaagg acggcggcca
3361 ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta
3421 ctactacgtg gactccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
3481 gcagtacgag cgcgccgagg gccgccacca cctgttcctg tagcggccaa ttctgcagtc
3541 gacatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
3601 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
3661 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
3721 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
3781 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
3841 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
3901 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
3961 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
4021 cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
4081 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caatcgatat
4141 ggctgagccc cgccaggagt tcgaagtgat ggaagatcac gctgggacgt acgggttggg
4201 ggacaggaaa gatcaggggg gctacaccat gcaccaagac caagagggtg acacggacgc
4261 tggcctgaaa gaatctcccc tgcagacccc cactgaggac ggatctgagg aaccgggctc
4321 tgaaacctct gatgctaaga gcactccaac agcggaagat gtgacagcac ccttagtgga
4381 tgagggagct cccggcaagc aggctgccgc gcagccccac acggagatcc cagaaggaac
4441 cacagctgaa gaagcaggca ttggagacac ccccagcctg gaagacgaag ctgctggtca
4501 cgtgacccaa gctcgcatgg tcagtaaaag caaagacggg actggaagcg atgacaaaaa
4561 agccaagggg gctgatggta aaacgaagat cgccacaccg cggggagcag cccctccagg
4621 ccagaagggc caggccaacg ccaccaggat tccagcaaaa accccgcccg ctccaaagac
4681 accacccagc tctggtgaac ctccaaaatc aggggatcgc agcggctaca gcagccccgg
4741 ctccccaggc actcccggca gccgctcccg caccccgtcc cttccaaccc cacccacccg
4801 ggagcccaag aaggtggcag tggtccgtac tccacccaag tcgccgtctt ccgccaagag
4861 ccgcctgcag acagcccccg tgcccatgcc agacctgaag aatgtcaagt ccaagatcgg
4921 ctccactgag aacctgaagc accagccggg aggcgggaag gtgcagataa ttaataagaa
4981 gctggatctt agcaacgtcc agtccaagtg tggctcaaag gataatatca aacacgtccc
5041 gggaggcggc agtgtgcaaa tagtctacaa accagttgac ctgagcaagg tgacctccaa
5101 gtgtggctca ttaggcaaca tccatcataa accaggaggt ggccaggtgg aagtaaaatc
5161 tgagaagctt gacttcaagg acagagtcca gtcgaagatt gggtccctgg acaatatcac
5221 ccacgtccct ggcggaggaa ataaaaagat tgaaacccac aagctgacct tccgcgagaa
5281 cgccaaagcc aagacagacc acggggcgga gatcgtgtac aagtcgccag tggtgtctgg
5341 ggacacgtct ccacggcatc tcagcaatgt ctcctccacc ggcagcatcg acatggtaga
5401 ctcgccccag ctcgccacgc tagctgacga ggtgtctgcc tccctggcca agcagggttt
5461 gctcgaggcg gccgctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
5521 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
5581 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
5641 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
5701 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
5761 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
5821 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
5881 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
5941 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
6001 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
6061 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
6121 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
6181 cgagctgtac aagtaatcta gataactgat cctctcccta tagtgagtcg tattaaattc
6241 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
6301 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
6361 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
6421 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
6481 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
6541 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
6601 aggggtgggt ccgggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
6661 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
6721 ccgggccttt cgacctgcag cccaagctta ccatgattga acaagatgga ttgcacgcag
6781 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacgatcg
6841 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
6901 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
6961 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
7021 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
7081 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
7141 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
7201 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
7261 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
7321 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
7381 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
7441 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
7501 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgacct gaggatcata
7561 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
7621 ctgaacctga aacataaaat gaatgcaatt gatagatcct aatcaacctc tggattacaa
7681 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
7741 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
7801 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
7861 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
7921 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
7981 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
8041 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
8101 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
8161 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
8221 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
8281 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
8341 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
8401 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
8461 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
8521 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
8581 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
8641 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
8701 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
8761 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
8821 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
8881 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
8941 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
9001 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
9061 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
9121 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
9181 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
9241 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
9301 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
9361 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
9421 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
9481 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
9541 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
9601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
9661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
9721 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
9781 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
9841 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
9901 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
9961 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
10021 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
10081 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
10141 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
10201 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
10261 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
10321 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
10381 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
10441 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
10501 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
10561 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
10621 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
10681 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
10741 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
10801 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
10861 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
10921 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
10981 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
11041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
11101 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
11161 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
11221 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagta
//
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