pAX01-ComK

LOCUS       pAX01-ComK                8378 bp ds-DNA     circular SYN 06-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   biofeng.com
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 6, 2023 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      546..580
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      660..687
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      706..792
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2079..2183
                     /gene="bla"
                     /label=AmpR promoter
     terminator      4271..4305
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     promoter        4949..5053
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5054..5914
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6085..6673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6859..6999
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(7101..7292)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggggtgatgt caaagcttga aaaaacgcac gtaacaaaag caaaatttat gctccatggg
       61 ggagactaca accccgatca gtggctggat cggcccgata ttttagctga cgatatcaaa
      121 ctgatgaagc tttctcatac gaatacgttt tctgtcggca tttttgcatg gagcgcactt
      181 gagccggagg agggcgtata tcaatttgaa tggctggatg atatttttga gcggattcac
      241 agtataggcg gccgggtcat attagcaacg ccgagcggag cccgtccggc ctggctgtcg
      301 caaacctatc cggaagtttt gcgcgtcaat gcctcccgcg tcaaacagct gcacggcgga
      361 aggcacaacc actgcctcac atctaaagtc taccgagaaa aaacacggca catcaaccgc
      421 ttattagcag aacgatacgg acatcacccg gcgctgttaa tgtggcacat ttcaaacgaa
      481 tacgggggag attgccactg tgaatcgatg cggccgctct agagcaacgt tcttgccatt
      541 gctgcataaa aaacgcccgg cggcaaccga gcgttctgaa ttaattaatc atcgggaaga
      601 tcttcatcac cgaaacgcgg caggcagctc tagagttaac aagagtttgt agaaacgcaa
      661 aaaggccatc cgtcaggatg gccttctgct tagctagagc ggcggatttg tcctactcag
      721 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
      781 tcgttttatt tgatgcctca agctagagag tcattaccag atctcactgc agagatcccc
      841 gggtctagag tctagggacc tctttagctc cttggaagct gtcagtagta tacctaataa
      901 tttatctaca ttccctttag taacgtgtaa ctttccaaat ttacaaaagc gactcataga
      961 attatttcct cccgttaaat aatagataac tattaaaaat agacaatact tgctcataag
     1021 taacggtact taaattgttt actttggcgt gtttcattgc ttgatgaaac tgatttttag
     1081 taaacagttg acgatattct cgattgaccc attttgaaac aaagtacgta tatagcttcc
     1141 aatatttatc tggaacatct gtggtatggc gggtaagttt tattaagaca ctgtttactt
     1201 ttggtttagg atgaaagcat tccgctggca gcttaagcaa ttgctgaatc gagacttgag
     1261 tgtgcaagag caaccctagt gttcggtgaa tatccaaggt acgcttgtag aatccttctt
     1321 caacaatcag atagatgtca gacgcatggc tttcaaaaac cactttttta ataatttgtg
     1381 tgcttaaatg gtaaggaata ctcccaacaa ttttatacct ctgtttgtta gggaattgaa
     1441 actgtagaat atcttggtga attaaagtga cacgagtatt cagttttaat ttttctgacg
     1501 ataagttgaa tagatgactg tctaattcaa tagacgttac ctgtttactt attttagcca
     1561 gtttcgtcgt taaatgccct ttacctgttc caatttcgta aacggtatcg gtttctttta
     1621 aattcaattg ttttattatt tggttgagta ctttttcact cgttaaaaag ttttgagaat
     1681 attttatatt tttgttcatg taatcactcc ttcttaatta caaattttta gcatctaatt
     1741 taacttcaat tcctattata caaaatttta agatactgca ctatcaacac actcttaagt
     1801 ttgcttctaa gtcttatttc cataacttct tttacgtttc cgccattctt tgctgtttcg
     1861 atttttatga tatggtgcaa gtcagcacga acacgaaccg tcttatctcc cattatatct
     1921 ttttttgcac tgattggtgt atcatttcgt ttttcttttt gtgctagagg atcaattctt
     1981 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     2041 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2101 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2161 aataatattg aaaaaggaag agtgcggccg cccgcggcta tttttctaat accgttcccc
     2221 gagctcacgc aaaataaaat cataaatcat cttcgtccgc tcttctttcg ggtacagcat
     2281 aaattcctgt cttttcggca cgcggtggtc gattctgtct tggaatttgg ttctgagcca
     2341 cgctgttcgg tatacctggt tctcgaacga attataagag atcggcagct ccatcgtttt
     2401 cccattggaa aacgtcactt ccgtatcgtc gaattcagtc gctttgaatt cttttacatg
     2461 cacatgggaa atccagccgc attggggtct tgtcgaagaa agtgtaggga ataaaaagat
     2521 ttggttcgaa gggtccacca tgatcggcgg cttgtgtgaa atttttgtca cttcataagt
     2581 tcctgctttt cttcccgcat agcttgatcc aaaaaatcgg cagcttctgt cgacaatttg
     2641 cagcggcttc atgtttacat aaaacacgca atctttttca ataattttgg aacagatttt
     2701 gccgtctatt tcttctggca gcacggcaat tgttgcgccg ttcacttcat acgattctaa
     2761 aggtgcgtct gttttctgac tcatattatg gcctcctttg gatcccattt ccccctttga
     2821 tttttagata tcactagttt ggaccatttg tcatttcccc ctttgattta agtgaacaag
     2881 tttatccatc aactatctta attgagttag tttgtttatc caataaacta actttatctc
     2941 atcatataca aaataaatgt ttatttcaat gtttttttta gaaaatttag ttataatatt
     3001 agatatgata cttttaaata tctaattcaa gcttcaaaaa acaccaactt agttcggtgg
     3061 ataaacaaag gagtggttat tattcaaatt gcagatcaag ctttagtaaa aaaaatgaat
     3121 caaaaattaa tattagatga aattttgaag aactcccctg tctccagggc aactctctct
     3181 gagattacag gattaaacaa gtctactgtc tcctctcaag taaatacact gcttgaaaaa
     3241 gattttattt ttgaaattgg ggcagggcaa tctagaggcg gcagaagacc tgtaatgctt
     3301 gtttttaata agaatgcagg ctactcgatt ggtattgata taggagtcga ctatcttaac
     3361 ggaattctaa ccgacttaga aggaaatatt attctcgaga agacttctga cttgtctagt
     3421 tcttccgcta gtgaagtaaa agagatttta tttgcactta ttcatggttt tgtaacccat
     3481 atgcctgagt ccccttatgg tctagtcgga ataggaattt gtgttccagg ccttgtagat
     3541 cgtcatcagc aaattatttt catgcctaac ttaaattgga atatcaaaga tttgcagttt
     3601 ttaattgaga gtgagtttaa tgttccggtt tttgttgaaa atgaagctaa tgcaggagca
     3661 tacggtgaaa aagtatttgg tatgacaaaa aactatgaaa acatcgttta catcagtatt
     3721 aatatcggaa ttggaactgg acttgttatt aacaacgaat tgtataaagg tgttcagggt
     3781 ttttctgggg aaatgggtca tatgacgata gattttaatg gacccaaatg cagctgtgga
     3841 aatcgaggct gttgggaatt atatgcttct gaaaaagcgt tactggcttc gctctctaaa
     3901 gaagaaaaga atatttctcg aaaagagatt gtggaacgcg caaataaaaa tgatgtagaa
     3961 atgttaaatg cacttcaaaa ctttggcttt tatatcggaa ttggattaac caatatcctt
     4021 aatacatttg atatagaagc tgttatcttg agaaatcata taattgaatc tcatcccatt
     4081 gttttaaata cgattaaaaa cgaagtttct tctagagtcc attctcattt agacaataaa
     4141 tgtgaactat tgccttcttc gttaggaaaa aatgcacctg ctttaggagc ggtttctatc
     4201 gttattgatt cttttttaag tgttacccct ataagttagg agctccccgg gacgttcttg
     4261 ccattgctgc ataaaaaacg cccggcggca accgagcgtt ctgaattaat taatcatcgc
     4321 gactgcagag atatcgattt caagctatat ttggagttga gcctcttgaa acggacaccc
     4381 tgtatccgaa ggatcgaaac gctgtcagct accgcagcca aatatatgaa atgaaggatt
     4441 atgcaaccgt gattgatgta aagacagctt cagtggaagc ggtgtatcaa gaagattttt
     4501 atgcgcgcac gccagcggtc acaagccatg agtatcagca gggcaaggcg tattttatcg
     4561 gcgcgcgttt ggaggatcaa tttcagcgtg atttctatga gggtctgatc acagacctgt
     4621 ctctctctcc agtttttccg gttcggcacg gaaaaggcgt ctccgtacaa gcgaggcagg
     4681 atcaggacaa tgattatatt tttgtcatga atttcacgga agaaaaacag ctggtcacgt
     4741 ttgatcagag tgtgaaggac ataatgacag gagacatatt gtcaggcgac ctgacgatgg
     4801 aaaagtatga agtgagaatt gtcgtaaaca cacattagcc catcattctt gaagacgaaa
     4861 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4921 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4981 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5041 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5101 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5161 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5221 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5281 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     5341 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5401 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5461 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5521 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5581 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5641 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5701 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5761 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5821 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5881 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5941 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6001 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6061 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6121 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6181 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6241 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6301 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6361 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6421 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     6481 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6541 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6601 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6661 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6721 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6781 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6841 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     6901 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     6961 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     7021 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7081 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     7141 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     7201 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     7261 atgttaaggg cggttttttc ctgtttggtc acttgatgcc tccgtgtaag ggggaatttc
     7321 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
     7381 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
     7441 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
     7501 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
     7561 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg
     7621 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
     7681 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
     7741 gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc
     7801 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag
     7861 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc
     7921 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag
     7981 gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga
     8041 ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc
     8101 cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc
     8161 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat
     8221 ccagcctcgc gtcgcgacta agaaaatgcc gtcaaatccg ctcgccatga cttcactaac
     8281 gatgcctttg aaaatcttca agttcttttc tactaattca aggcgtgtct caccaggttt
     8341 ttggtttgct ccggcgcaaa tgcagacaat atcaggat
//