| 质粒名称: | Fuw-dCas9-Dnmt3a_IM |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 84478 |
| 存储实验室: | Rudolf Jaenisch |
|
载体骨架基本信息 |
|
| 载体名称: | FUW |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9207 bp |
| 完整质粒大小: | 14313 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | Zeocin; 抗性标记不在LTR内，不会包装到病毒中 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCas9-Dnmt3a_IM |
| 物种来源: | H. sapiens (human), Synthetic |
| 插入大小: | 5106 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AscI (未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | aactatgcgctcggg |
| 3' 测序引物 | taaagcagcgtatcc |
|
文献引用方法 |
|
| 材料和方法部分: | Fuw-dCas9-Dnmt3a_IM was a gift from Rudolf Jaenisch (Addgene plasmid # 84478 ; http://n2t.net/addgene:84478 ; RRID:Addgene_84478) |
| 参考文献部分: | Editing DNA Methylation in the Mammalian Genome. Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R. Cell. 2016 Sep 22;167(1):233-247.e17. doi: 10.1016/j.cell.2016.08.056. 10.1016/j.cell.2016.08.056 PubMed 27662091 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Fuw-dCas9-Dnmt3a_IM 14241 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Lentiviral construct to express dCas9 fused with an inactive Dnmt3a
catalytic domain (E664A, E756A).
ACCESSION .
VERSION .
KEYWORDS Fuw-dCas9-Dnmt3a_IM
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14241)
AUTHORS Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D,
Young RA, Jaenisch R
TITLE Editing DNA Methylation in the Mammalian Genome.
JOURNAL Cell. 2016 Sep 22;167(1):233-247.e17. doi:
10.1016/j.cell.2016.08.056.
PUBMED 27662091
REFERENCE 2 (bases 1 to 14241)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..14241
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 91..470
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 472..670
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 625..645
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 688..868
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 915..1040
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1533..1766
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2293..2410
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2475..3686
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 3523..3542
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 3737..7840
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 7853..7873
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7877..7897
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7901..7921
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 8858..9446
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8911..8931)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(9329..9340)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(9449..9465)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9450..9466)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 9971..10151
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(10177..10194)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 10183..10407
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 10453..10881
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(10540..10559)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 10750..10771
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(10890..10910)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 10934..11144
/label=SV40 promoter
/note="SV40 early promoter"
promoter 10956..11152
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 11003..11138
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 11065..11084
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 11200..11247
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 11266..11640
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 11770..11891
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11807..11826)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 11861..11880
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(11940..11956)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11940..11956)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11953..11975)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11964..11980
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11988..12018)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 12033..12054
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(12171..12188)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(12342..12930)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(12422..12441)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(13101..13961)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 13724..13743
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13962..14066)
/gene="bla"
/label=AmpR promoter
primer_bind complement(14141..14160)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
61 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
121 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
181 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
241 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
301 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
481 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
541 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
601 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
661 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
721 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
781 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
841 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
901 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
961 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
1021 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
1081 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
1141 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
1201 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
1261 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
1321 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
1381 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
1441 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
1501 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
1561 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
1621 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
1681 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
1741 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
1801 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
1861 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
1921 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
1981 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
2041 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
2101 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
2161 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
2221 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
2281 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
2341 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
2401 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttaacccgtg
2461 tcggctccag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
2521 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
2581 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
2641 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
2701 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
2761 cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca
2821 gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag
2881 cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg
2941 tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg
3001 ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt
3061 gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa
3121 ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
3181 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
3241 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
3301 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
3361 tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
3421 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
3481 agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
3541 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
3601 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
3661 ggccgttttt ggcttttttg ttagacgaag cttgggctgc aggtcgactc tagaagttaa
3721 cctcgagggc gcgcccatgg acaagaagta ttctatcgga ctggccatcg ggactaatag
3781 cgtcgggtgg gccgtgatca ctgacgagta caaggtgccc tctaagaagt tcaaggtgct
3841 cgggaacacc gaccggcatt ccatcaagaa aaatctgatc ggagctctcc tctttgattc
3901 aggggagacc gctgaagcaa cccgcctcaa gcggactgct agacggcggt acaccaggag
3961 gaagaaccgg atttgttacc ttcaagagat attctccaac gaaatggcaa aggtcgacga
4021 cagcttcttc cataggctgg aagaatcatt cctcgtggaa gaggataaga agcatgaacg
4081 gcatcccatc ttcggtaata tcgtcgacga ggtggcctat cacgagaaat acccaaccat
4141 ctaccatctt cgcaaaaagc tggtggactc aaccgacaag gcagacctcc ggcttatcta
4201 cctggccctg gcccacatga tcaagttcag aggccacttc ctgatcgagg gcgacctcaa
4261 tcctgacaat agcgatgtgg ataaactgtt catccagctg gtgcagactt acaaccagct
4321 ctttgaagag aaccccatca atgcaagcgg agtcgatgcc aaggccattc tgtcagcccg
4381 gctgtcaaag agccgcagac ttgagaatct tatcgctcag ctgccgggtg aaaagaaaaa
4441 tggactgttc gggaacctga ttgctctttc acttgggctg actcccaatt tcaagtctaa
4501 tttcgacctg gcagaggatg ccaagctgca actgtccaag gacacctatg atgacgatct
4561 cgacaacctc ctggcccaga tcggtgacca atacgccgac cttttccttg ctgctaagaa
4621 tctttctgac gccatcctgc tgtctgacat tctccgcgtg aacactgaaa tcaccaaggc
4681 ccctctttca gcttcaatga ttaagcggta tgatgagcac caccaggacc tgaccctgct
4741 taaggcactc gtccggcagc agcttccgga gaagtacaag gaaatcttct ttgaccagtc
4801 aaagaatgga tacgccggct acatcgacgg aggtgcctcc caagaggaat tttataagtt
4861 tatcaaacct atccttgaga agatggacgg caccgaagag ctcctcgtga aactgaatcg
4921 ggaggatctg ctgcggaagc agcgcacttt cgacaatggg agcattcccc accagatcca
4981 tcttggggag cttcacgcca tccttcggcg ccaagaggac ttctacccct ttcttaagga
5041 caacagggag aagattgaga aaattctcac tttccgcatc ccctactacg tgggacccct
5101 cgccagagga aatagccggt ttgcttggat gaccagaaag tcagaagaaa ctatcactcc
5161 ctggaacttc gaagaggtgg tggacaaggg agccagcgct cagtcattca tcgaacggat
5221 gactaacttc gataagaacc tccccaatga gaaggtcctg ccgaaacatt ccctgctcta
5281 cgagtacttt accgtgtaca acgagctgac caaggtgaaa tatgtcaccg aagggatgag
5341 gaagcccgca ttcctgtcag gcgaacaaaa gaaggcaatt gtggaccttc tgttcaagac
5401 caatagaaag gtgaccgtga agcagctgaa ggaggactat ttcaagaaaa ttgaatgctt
5461 cgactctgtg gagattagcg gggtcgaaga tcggttcaac gcaagcctgg gtacctacca
5521 tgatctgctt aagatcatca aggacaagga ttttctggac aatgaggaga acgaggacat
5581 ccttgaggac attgtcctga ctctcactct gttcgaggac cgggaaatga tcgaggagag
5641 gcttaagacc tacgcccatc tgttcgacga taaagtgatg aagcaactta aacggagaag
5701 atataccgga tggggacgcc ttagccgcaa actcatcaac ggaatccggg acaaacagag
5761 cggaaagacc attcttgatt tccttaagag cgacggattc gctaatcgca acttcatgca
5821 acttatccat gatgattccc tgacctttaa ggaggacatc cagaaggccc aagtgtctgg
5881 acaaggtgac tcactgcacg agcatatcgc aaatctggct ggttcacccg ctattaagaa
5941 gggtattctc cagaccgtga aagtcgtgga cgagctggtc aaggtgatgg gtcgccataa
6001 accagagaac attgtcatcg agatggccag ggaaaaccag actacccaga agggacagaa
6061 gaacagcagg gagcggatga aaagaattga ggaagggatt aaggagctcg ggtcacagat
6121 ccttaaagag cacccggtgg aaaacaccca gcttcagaat gagaagctct atctgtacta
6181 ccttcaaaat ggacgcgata tgtatgtgga ccaagagctt gatatcaaca ggctctcaga
6241 ctacgacgtg gacgccatcg tccctcagag cttcctcaaa gacgactcaa ttgacaataa
6301 ggtgctgact cgctcagaca agaaccgggg aaagtcagat aacgtgccct cagaggaagt
6361 cgtgaaaaag atgaagaact attggcgcca gcttctgaac gcaaagctga tcactcagcg
6421 gaagttcgac aatctcacta aggctgagag gggcggactg agcgaactgg acaaagcagg
6481 attcattaaa cggcaacttg tggagactcg gcagattact aaacatgtcg cccaaatcct
6541 tgactcacgc atgaatacca agtacgacga aaacgacaaa cttatccgcg aggtgaaggt
6601 gattaccctg aagtccaagc tggtcagcga tttcagaaag gactttcaat tctacaaagt
6661 gcgggagatc aataactatc atcatgctca tgacgcatat ctgaatgccg tggtgggaac
6721 cgccctgatc aagaagtacc caaagctgga aagcgagttc gtgtacggag actacaaggt
6781 ctacgacgtg cgcaagatga ttgccaaatc tgagcaggag atcggaaagg ccaccgcaaa
6841 gtacttcttc tacagcaaca tcatgaattt cttcaagacc gaaatcaccc ttgcaaacgg
6901 tgagatccgg aagaggccgc tcatcgagac taatggggag actggcgaaa tcgtgtggga
6961 caagggcaga gatttcgcta ccgtgcgcaa agtgctttct atgcctcaag tgaacatcgt
7021 gaagaaaacc gaggtgcaaa ccggaggctt ttctaaggaa tcaatcctcc ccaagcgcaa
7081 ctccgacaag ctcattgcaa ggaagaagga ttgggaccct aagaagtacg gcggattcga
7141 ttcaccaact gtggcttatt ctgtcctggt cgtggctaag gtggaaaaag gaaagtctaa
7201 gaagctcaag agcgtgaagg aactgctggg tatcaccatt atggagcgca gctccttcga
7261 gaagaaccca attgactttc tcgaagccaa aggttacaag gaagtcaaga aggaccttat
7321 catcaagctc ccaaagtata gcctgttcga actggagaat gggcggaagc ggatgctcgc
7381 ctccgctggc gaacttcaga agggtaatga gctggctctc ccctccaagt acgtgaattt
7441 cctctacctt gcaagccatt acgagaagct gaaggggagc cccgaggaca acgagcaaaa
7501 gcaactgttt gtggagcagc ataagcatta tctggacgag atcattgagc agatttccga
7561 gttttctaaa cgcgtcattc tcgctgatgc caacctcgat aaagtcctta gcgcatacaa
7621 taagcacaga gacaaaccaa ttcgggagca ggctgagaat atcatccacc tgttcaccct
7681 caccaatctt ggtgcccctg ccgcattcaa gtacttcgac accaccatcg accggaaacg
7741 ctatacctcc accaaagaag tgctggacgc caccctcatc caccagagca tcaccggact
7801 ttacgaaact cggattgacc tctcacagct cggaggggat gagggagctg atccaaaaaa
7861 gaagagaaag gtagacccaa agaaaaaacg aaaagtagat ccgaaaaaga agaggaaggt
7921 gggatccaac cacgaccagg aatttgaccc tccaaaggtt tacccacctg tcccagctga
7981 gaagaggaag cccatccggg tgctgtctct ctttgatgga atcgctacag ggctcctggt
8041 gctgaaggac ttgggcattc aggtggaccg ctacattgcc tcggccgtgt gtgaggactc
8101 catcacggtg ggcatggtgc ggcaccaggg gaagatcatg tacgtcgggg acgtccgcag
8161 cgtcacacag aagcatatcc aggagtgggg cccattcgat ctggtgattg ggggcagtcc
8221 ctgcaatgac ctctccatcg tcaaccctgc tcgcaagggc ctctacgagg gcactggccg
8281 gctcttcttt gagttctacc gcctcctgca tgatgcgcgg cccaaggagg gagatgatcg
8341 ccccttcttc tggctctttg ccaatgtggt ggccatgggc gttagtgaca agagggacat
8401 ctcgcgattt ctcgagtcca accctgtgat gattgatgcc aaagaagtgt cagctgcaca
8461 cagggcccgc tacttctggg gtaaccttcc cggtatgaac aggccgttgg catccactgt
8521 gaatgataag ctggagctgc aggagtgtct ggagcatggc aggatagcca agttcagcaa
8581 agtgaggacc attactacga ggtcaaactc cataaagcag ggcaaagacc agcattttcc
8641 tgtcttcatg aatgagaaag aggacatctt atggtgcact gaaatggaaa gggtatttgg
8701 tttcccagtc cactatactg acgtctccaa catgagccgc ttggcgaggc agagactgct
8761 gggccggtca tggagcgtgc cagtcatccg ccacctcttc gctccgctga aggagtattt
8821 tgcgtgtgtg taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat
8881 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
8941 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
9001 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
9061 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
9121 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
9181 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
9241 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
9301 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
9361 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
9421 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
9481 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
9541 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
9601 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
9661 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
9721 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
9781 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
9841 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
9901 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
9961 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
10021 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
10081 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
10141 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
10201 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
10261 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
10321 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
10381 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
10441 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
10501 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
10561 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
10621 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
10681 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
10741 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
10801 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
10861 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
10921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
10981 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
11041 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
11101 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
11161 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
11221 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
11281 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
11341 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
11401 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
11461 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
11521 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
11581 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
11641 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
11701 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
11761 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
11821 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
11881 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
11941 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
12001 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
12061 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
12121 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
12181 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
12241 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
12301 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
12361 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
12421 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
12481 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
12541 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
12601 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
12661 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
12721 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
12781 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
12841 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
12901 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
12961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
13021 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
13081 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
13141 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
13201 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
13261 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
13321 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
13381 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
13441 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
13501 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
13561 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
13621 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
13681 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
13741 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
13801 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
13861 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
13921 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
13981 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
14041 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
14101 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
14161 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
14221 gcaaaattta agctacaaca a
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：bRA89
- 下一篇：pEP4 E02S EN2K
