| 质粒名称: | pLIB |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 80610 |
| 存储实验室: | Jan-Michael Peters |
|
载体骨架基本信息 |
|
| 载体名称: | pLIB |
| 载体出品公司: | -- |
| 空载体大小: | 4970 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 昆虫表达 |
| 筛选标记: | 庆大霉素 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | polyhedrin |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | TCAACAGGTTGAACTGCTGATC |
| 3’测序引物 | GGTGTAGCGTCGTAAGCTAATAC |
|
文献引用方法 |
|
| 材料和方法部分: | pLIB was a gift from Jan-Michael Peters (Addgene plasmid # 80610 ; http://n2t.net/addgene:80610 ; RRID:Addgene_80610) |
| 参考文献部分: | biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes. Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM. Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 10.1073/pnas.1604935113. Epub 2016 Apr 25. 10.1073/pnas.1604935113 PubMed 27114506 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLIB 4970 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pLIB - Library vector (biGBac system for expression of protein
complexes in insect cells).
ACCESSION .
VERSION .
KEYWORDS pLIB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4970)
AUTHORS Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown
NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
TITLE biGBac enables rapid gene assembly for the expression of large
multisubunit protein complexes.
JOURNAL Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi:
10.1073/pnas.1604935113. Epub 2016 Apr 25.
PUBMED 27114506
REFERENCE 2 (bases 1 to 4970)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4970
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 102..556
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(189..208)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 398..419
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 582..686
/gene="bla"
/label=AmpR promoter
CDS 687..1547
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(905..924)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1718..2306
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2207..2226
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2460..2477
/label=L4440
/note="L4440 vector, forward primer"
mobile_element 2611..2835
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (right end of the Tn7 transposon)"
CDS complement(2902..3435)
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/label=GmR
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
promoter complement(3624..3652)
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
protein_bind 4048..4081
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter 4129..4220
/gene="polh from Autographa californica"
/label=polyhedrin promoter
/note="promoter for the baculovirus polyhedrin gene"
primer_bind 4145..4162
/label=Polyhedrin-F
/note="Polyhedrin promoter, forward primer"
polyA_signal 4479..4613
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4516..4535)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4570..4589
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
mobile_element 4723..4888
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
3961 atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa ctgctgatca
4021 acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta tacgaagtta
4081 tctggtttta ctagtatcga ttcgcgacct actccggaat attaatagat catggagata
4141 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
4201 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
4261 cccggtccga agcgcgcgga attcaaaggc ctacgtcgac gagctcacta gtcgcggccg
4321 ctttcgaatc tagagcctgc agtctcgaca agcttgtcga gaagtactag aggatcataa
4381 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
4441 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
4501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
4561 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac
4621 tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
4681 gccaccgctg agcaataact atcataaccc ctaggagatc cgaaccagat aagtgaaatc
4741 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt
4801 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc
4861 cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa
4921 tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
//
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