| 质粒名称: | PUAS-Luc2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 24343 |
| 存储实验室: | Liqun Luo |
|
载体骨架基本信息 |
|
| 载体名称: | pGL4.23 |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4283 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Luciferase |
| 标签/融合蛋白: | 5XGAL4 (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | LucNRev |
|
文献引用方法 |
|
| 材料和方法部分: | pUAS-luc2 was a gift from Liqun Luo (爱迪基因 plasmid # 24343) |
| 参考文献部分: | The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pUAS-luc2 4623 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pUAS-luc2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4623)
AUTHORS Potter CJ, Tasic B, Russler EV, Liang L, Luo L.
TITLE The Q System: A Repressible Binary System for Transgene Expression,
Lineage Tracing, and Mosaic Analysis
JOURNAL Volume 141, Issue 3, 536-548, 30 April 2010
PUBMED 20434990
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|PUAS-Luc2|
FEATURES Location/Qualifiers
source 1..4623
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 85..179
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 198..436
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
primer_bind 383..404
/label=pCasper-hs
/note="Drosophila Hsp70 promoter, forward primer"
regulatory 475..484
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 481..2133
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 2177..2298
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2188..2207)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2242..2261
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2546..2563)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2717..3305)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2797..2816)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3505..4365)
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4470..4518
/note="synthetic polyadenylation signal"
misc_feature 4532..4623
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4572..4591
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg
121 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
181 ggagactcta gcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc
241 aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca
301 gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa
361 ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata
421 caagaagaga actctgaata gggaattggg aattcgttaa cagatccgat atccgccacc
481 atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact cgaagacggg
541 accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc cggcaccatc
601 gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt cgagatgagc
661 gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg gatcgtggtg
721 tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg
781 gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag catgggcatc
841 agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct caacgtgcaa
901 aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga ctaccagggc
961 ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa cgagtacgac
1021 ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa cagtagtggc
1081 agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt ccgattcagt
1141 catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat cctcagcgtg
1201 gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat ctgcggcttt
1261 cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt gcaagactat
1321 aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa gagcactctc
1381 atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc gccgctcagc
1441 aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg ccagggctac
1501 ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga caagcctggc
1561 gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga caccggtaag
1621 acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat catgagcggc
1681 tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg gctgcacagc
1741 ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg gctgaagagc
1801 ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat cctgctgcaa
1861 caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc cggcgagctg
1921 cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga gatcgtggac
1981 tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt gttcgtggac
2041 gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga gattctcatt
2101 aaggccaaga agggcggcaa gatcgccgtg taataattct agagtcgggg cggccggccg
2161 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
2221 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
2281 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
2341 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
2401 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
2461 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
2521 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
2581 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
2641 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
2701 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
2761 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
2821 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
2881 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
2941 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
3001 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
3061 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
3121 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
3181 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
3241 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
3301 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
3361 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
3421 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc
3481 gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc
3541 gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat
3601 tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc
3661 ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc
3721 tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag
3781 ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg
3841 ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg
3901 atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag
3961 taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt
4021 catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga
4081 gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc
4141 acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc
4201 aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc
4261 ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc
4321 cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca
4381 atattattga agcatttatc agggttacta gtacgtctct caaggataag taagtaatat
4441 taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt
4501 gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga
4561 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc
4621 tct
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pGP-CMV-Gcamp6f
- 下一篇：pQUAST>stop>mCD8-GFP
