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pCEP4-DsRed2载体质粒基本信息
pCEP4-DsRed2质粒图谱载体图谱和pCEP4-DsRed2载体序列质粒序列多克隆位点信息
pCEP4-DsRed2质粒载体简介
pCEP4-DsRed2质粒序列载体序列
LOCUS pCEP4-DsRed2 10860 bp DNA circular SYN 12-JUL-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10860) AUTHORS Biofeng TITLE Direct Submission JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2 https://www.biofeng.com FEATURES Location/Qualifiers source 1..10860 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 4..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 384..587 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" regulatory 626..635 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 632..1309 /codon_start=1 /product="improved tetrameric variant of DsRed fluorescent protein" /label=DsRed2 /note="mammalian codon-optimized" /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH LFL" polyA_signal 1346..1480 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 2203..3992 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" CDS complement(4294..6219) /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" promoter 6740..6844 /gene="bla" /label=AmpR promoter CDS 6845..7705 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 7876..8464 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 8895..9040 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" CDS 9083..10120 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE MGEAN" polyA_signal 10162..10210 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" ORIGIN 1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat 421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc 541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc 601 gtcagatctc tagaagctgg gtaccgccac catggcctcc tccgagaacg tcatcaccga 661 gttcatgcgc ttcaaggtgc gcatggaggg caccgtgaac ggccacgagt tcgagatcga 721 gggcgagggc gagggccgcc cctacgaggg ccacaacacc gtgaagctga aggtgaccaa 781 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa 841 ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg 901 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggcgaccg tgacccagga 961 ctcctccctg caggacggct gcttcatcta caaggtgaag ttcatcggcg tgaacttccc 1021 ctccgacggc cccgtgatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct 1081 gtacccccgc gacggcgtgc tgaagggcga gacccacaag gccctgaagc tgaaggacgg 1141 cggccactac ctggtggagt tcaagtccat ctacatggcc aagaagcccg tgcagctgcc 1201 cggctactac tacgtggacg ccaagctgga catcacctcc cacaacgagg actacaccat 1261 cgtggagcag tacgagcgca ccgagggccg ccaccacctg ttcctgtaga agcttgctag 1321 cggccgctcg aggccggcaa ggccggatcc agacatgata agatacattg atgagtttgg 1381 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 1441 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 1501 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 1561 caaatgtggt atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa 1621 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 1681 agcagacaag cccgtcaggc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg 1741 tcgactctag aggatcgatg ccccgccccg gacgaactaa acctgactac gacatctctg 1801 ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact 1861 gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta 1921 gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga 1981 gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg 2041 aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga 2101 ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag 2161 ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt 2221 cccgggtagt agtatatact atccagacta accctaattc aatagcatat gttacccaac 2281 gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc 2341 ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg agggtagtga 2401 accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga 2461 atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga 2521 acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa 2581 atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac 2641 cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa 2701 atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct 2761 atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc 2821 agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt 2881 ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg 2941 ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg 3001 gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg 3061 gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca 3121 aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg 3181 gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca 3241 gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg 3301 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta 3361 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta 3421 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta 3481 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta 3541 gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta 3601 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat 3661 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat 3721 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat 3781 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat 3841 atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat 3901 atgataccca gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg 3961 ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc tcccattctt aggtgaattt 4021 aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg 4081 ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc 4141 caattgcccc atgttgggag gacgaaaatg gtgacaagac agatggccag aaatacacca 4201 acagcacgca tgatgtctac tggggattta ttctttagtg cgggggaata cacggctttt 4261 aatacgattg agggcgtctc ctaacaagtt acatcactcc tgcccttcct caccctcatc 4321 tccatcacct ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag ccccttccac 4381 cataggtgga aaccagggag gcaaatctac tccatcgtca aagctgcaca cagtcaccct 4441 gatattgcag gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac 4501 ctcagcaaat atatgagttt gtaaaaagac catgaaataa cagacaatgg actcccttag 4561 cgggccaggt tgtgggccgg gtccaggggc cattccaaag gggagacgac tcaatggtgt 4621 aagacgacat tgtggaatag caagggcagt tcctcgcctt aggttgtaaa gggaggtctt 4681 actacctcca tatacgaaca caccggcgac ccaagttcct tcgtcggtag tcctttctac 4741 gtgactccta gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga 4801 acctccttga ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc 4861 ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca tctgcggggc cctgctctat 4921 cgctcccggg ggcacgtcag gctcaccatc tgggccacct tcttggtggt attcaaaata 4981 atcggcttcc cctacagggt ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg 5041 agacccggat gatgatgact gactactggg actcctgggc ctcttttctc cacgtccacg 5101 acctctcccc ctggctcttt cacgacttcc ccccctggct ctttcacgtc ctctaccccg 5161 gcggcctcca ctacctcctc gaccccggcc tccactacct cctcgacccc ggcctccact 5221 gcctcctcga ccccggcctc cacctcctgc tcctgcccct cctgctcctg cccctcctcc 5281 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc 5341 tcctgcccct cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgcccc 5401 tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc 5461 tgcccctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc 5521 tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgctcc 5581 tgcccctcct gcccctcctg cccctcctgc tcctgcccct cctcctgctc ctgcccctcc 5641 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc 5701 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc 5761 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc 5821 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgcccc 5881 tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcccgctcc 5941 tgctcctgct cctgttccac cgtgggtccc tttgcagcca atgcaacttg gacgtttttg 6001 gggtctccgg acaccatctc tatgtcttgg ccctgatcct gagccgcccg gggctcctgg 6061 tcttccgcct cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat caccccctct 6121 tctttgaggt ccactgccgc cggagccttc tggtccagat gtgtctccct tctctcctag 6181 gccatttcca ggtcctgtac ctggcccctc gtcagacatg attcacacta aaagagatca 6241 atagacatct ttattagacg acgctcagtg aatacaggga gtgcagactc ctgccccctc 6301 caacagcccc cccaccctca tccccttcat ggtcgctgtc agacagatcc aggtctgaaa 6361 attccccatc ctccgaacca tcctcgtcct catcaccaat tactcgcagc ccggaaaact 6421 cccgctgaac atcctcaaga tttgcgtcct gagcctcaag ccaggcctca aattcctcgt 6481 cccccttttt gctggacggt agggatgggg attctcggga cccctcctct tcctcttcaa 6541 ggtcaccaga cagagatgct actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg 6601 atcagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt 6661 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 6721 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 6781 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 6841 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 6901 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 6961 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 7021 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 7081 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 7141 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 7201 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 7261 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 7321 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 7381 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 7441 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 7501 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 7561 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 7621 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 7681 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 7741 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 7801 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 7861 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 7921 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 7981 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 8041 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 8101 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 8161 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 8221 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 8281 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 8341 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 8401 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 8461 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttgaagctg 8521 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat 8581 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc 8641 cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc gcgtgcggct gctggagatg 8701 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag 8761 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccctgcttca 8821 tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg 8881 tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca 8941 cgcagatgca gtcggggcgg cgcggtccga ggtccacttc gcatattaag gtgacgcgtg 9001 tggcctcgaa caccgagcga ccctgcagcg acccgcttaa cagcgtcaac agcgtgccgc 9061 agatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa 9121 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga 9181 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg 9241 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc 9301 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg 9361 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca 9421 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt 9481 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc 9541 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc 9601 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca 9661 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc 9721 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt 9781 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca 9841 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca 9901 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg 9961 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag 10021 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc 10081 cagcactcgt ccggatcggg agatggggga ggctaactga aacacggaag gagacaatac 10141 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg 10201 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg 10261 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt 10321 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagccact 10381 ggccccgtgg gttagggacg gggtccccca tggggaatgg tttatggttc gtgggggtta 10441 ttattttggg cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca gacccatggt 10501 ttttggatgg cctgggcatg gaccgcatgt actggcgcga cacgaacacc gggcgtctgt 10561 ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg atttctggcg tgccaagcta 10621 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat 10681 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat 10741 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata 10801 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgccat //
