pCEP4-DsRed2

LOCUS       pCEP4-DsRed2               10860 bp DNA     circular SYN 12-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Biofeng
  TITLE     Direct Submission
  JOURNAL   Exported Jul 12, 2023 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      626..635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             632..1309
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     polyA_signal    1346..1480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2203..3992
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4294..6219)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     promoter        6740..6844
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6845..7705
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7876..8464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        8895..9040
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9083..10120
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     polyA_signal    10162..10210
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccgccac catggcctcc tccgagaacg tcatcaccga
      661 gttcatgcgc ttcaaggtgc gcatggaggg caccgtgaac ggccacgagt tcgagatcga
      721 gggcgagggc gagggccgcc cctacgaggg ccacaacacc gtgaagctga aggtgaccaa
      781 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa
      841 ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg
      901 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggcgaccg tgacccagga
      961 ctcctccctg caggacggct gcttcatcta caaggtgaag ttcatcggcg tgaacttccc
     1021 ctccgacggc cccgtgatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     1081 gtacccccgc gacggcgtgc tgaagggcga gacccacaag gccctgaagc tgaaggacgg
     1141 cggccactac ctggtggagt tcaagtccat ctacatggcc aagaagcccg tgcagctgcc
     1201 cggctactac tacgtggacg ccaagctgga catcacctcc cacaacgagg actacaccat
     1261 cgtggagcag tacgagcgca ccgagggccg ccaccacctg ttcctgtaga agcttgctag
     1321 cggccgctcg aggccggcaa ggccggatcc agacatgata agatacattg atgagtttgg
     1381 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1441 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1501 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     1561 caaatgtggt atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa
     1621 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     1681 agcagacaag cccgtcaggc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
     1741 tcgactctag aggatcgatg ccccgccccg gacgaactaa acctgactac gacatctctg
     1801 ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact
     1861 gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta
     1921 gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga
     1981 gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg
     2041 aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga
     2101 ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag
     2161 ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt
     2221 cccgggtagt agtatatact atccagacta accctaattc aatagcatat gttacccaac
     2281 gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc
     2341 ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg agggtagtga
     2401 accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga
     2461 atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga
     2521 acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa
     2581 atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac
     2641 cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa
     2701 atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct
     2761 atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc
     2821 agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt
     2881 ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg
     2941 ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg
     3001 gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg
     3061 gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca
     3121 aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg
     3181 gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca
     3241 gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg
     3301 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     3361 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     3421 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     3481 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     3541 gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta
     3601 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
     3661 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     3721 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     3781 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     3841 atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat
     3901 atgataccca gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg
     3961 ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc tcccattctt aggtgaattt
     4021 aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg
     4081 ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc
     4141 caattgcccc atgttgggag gacgaaaatg gtgacaagac agatggccag aaatacacca
     4201 acagcacgca tgatgtctac tggggattta ttctttagtg cgggggaata cacggctttt
     4261 aatacgattg agggcgtctc ctaacaagtt acatcactcc tgcccttcct caccctcatc
     4321 tccatcacct ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag ccccttccac
     4381 cataggtgga aaccagggag gcaaatctac tccatcgtca aagctgcaca cagtcaccct
     4441 gatattgcag gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac
     4501 ctcagcaaat atatgagttt gtaaaaagac catgaaataa cagacaatgg actcccttag
     4561 cgggccaggt tgtgggccgg gtccaggggc cattccaaag gggagacgac tcaatggtgt
     4621 aagacgacat tgtggaatag caagggcagt tcctcgcctt aggttgtaaa gggaggtctt
     4681 actacctcca tatacgaaca caccggcgac ccaagttcct tcgtcggtag tcctttctac
     4741 gtgactccta gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga
     4801 acctccttga ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc
     4861 ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca tctgcggggc cctgctctat
     4921 cgctcccggg ggcacgtcag gctcaccatc tgggccacct tcttggtggt attcaaaata
     4981 atcggcttcc cctacagggt ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg
     5041 agacccggat gatgatgact gactactggg actcctgggc ctcttttctc cacgtccacg
     5101 acctctcccc ctggctcttt cacgacttcc ccccctggct ctttcacgtc ctctaccccg
     5161 gcggcctcca ctacctcctc gaccccggcc tccactacct cctcgacccc ggcctccact
     5221 gcctcctcga ccccggcctc cacctcctgc tcctgcccct cctgctcctg cccctcctcc
     5281 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     5341 tcctgcccct cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgcccc
     5401 tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc
     5461 tgcccctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc
     5521 tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgctcc
     5581 tgcccctcct gcccctcctg cccctcctgc tcctgcccct cctcctgctc ctgcccctcc
     5641 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     5701 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     5761 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc
     5821 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgcccc
     5881 tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcccgctcc
     5941 tgctcctgct cctgttccac cgtgggtccc tttgcagcca atgcaacttg gacgtttttg
     6001 gggtctccgg acaccatctc tatgtcttgg ccctgatcct gagccgcccg gggctcctgg
     6061 tcttccgcct cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat caccccctct
     6121 tctttgaggt ccactgccgc cggagccttc tggtccagat gtgtctccct tctctcctag
     6181 gccatttcca ggtcctgtac ctggcccctc gtcagacatg attcacacta aaagagatca
     6241 atagacatct ttattagacg acgctcagtg aatacaggga gtgcagactc ctgccccctc
     6301 caacagcccc cccaccctca tccccttcat ggtcgctgtc agacagatcc aggtctgaaa
     6361 attccccatc ctccgaacca tcctcgtcct catcaccaat tactcgcagc ccggaaaact
     6421 cccgctgaac atcctcaaga tttgcgtcct gagcctcaag ccaggcctca aattcctcgt
     6481 cccccttttt gctggacggt agggatgggg attctcggga cccctcctct tcctcttcaa
     6541 ggtcaccaga cagagatgct actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg
     6601 atcagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt
     6661 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6721 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6781 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6841 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6901 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6961 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7021 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7081 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7141 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7201 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7261 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7321 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7381 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7441 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7501 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7561 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7621 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7681 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7741 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7801 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7861 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7921 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7981 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     8041 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8101 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8161 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8221 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8281 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8341 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8401 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8461 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttgaagctg
     8521 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat
     8581 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc
     8641 cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc gcgtgcggct gctggagatg
     8701 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag
     8761 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccctgcttca
     8821 tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg
     8881 tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca
     8941 cgcagatgca gtcggggcgg cgcggtccga ggtccacttc gcatattaag gtgacgcgtg
     9001 tggcctcgaa caccgagcga ccctgcagcg acccgcttaa cagcgtcaac agcgtgccgc
     9061 agatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     9121 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     9181 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     9241 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     9301 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     9361 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     9421 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     9481 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     9541 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     9601 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     9661 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     9721 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     9781 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     9841 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     9901 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     9961 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
    10021 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
    10081 cagcactcgt ccggatcggg agatggggga ggctaactga aacacggaag gagacaatac
    10141 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg
    10201 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
    10261 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
    10321 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagccact
    10381 ggccccgtgg gttagggacg gggtccccca tggggaatgg tttatggttc gtgggggtta
    10441 ttattttggg cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca gacccatggt
    10501 ttttggatgg cctgggcatg gaccgcatgt actggcgcga cacgaacacc gggcgtctgt
    10561 ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg atttctggcg tgccaagcta
    10621 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
    10681 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
    10741 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
    10801 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgccat
//