| 质粒名称: | pLVX-M-puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 125839Boyi Gan |
| Vladislav Verkhusha | |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-puro |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 8041bp |
| 载体类型: | Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 标记： | Puromycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 无 |
|
文献引用方法 |
|
| 材料和方法部分: |
pLVX-M-puro was a gift from Boyi Gan (Addgene plasmid # 125839 ; http://n2t.net/addgene:125839 ; RRID:Addgene_125839)
|
| 参考文献部分: | BAP1 links metabolic regulation of ferroptosis to tumour suppression. Zhang Y, Shi J, Liu X, Feng L, Gong Z, Koppula P, Sirohi K, Li X, Wei Y, Lee H, Zhuang L, Chen G, Xiao ZD, Hung MC, Chen J, Huang P, Li W, Gan B. Nat Cell Biol. 2018 Oct;20(10):1181-1192. doi: 10.1038/s41556-018-0178-0. Epub 2018 Sep 10. 10.1038/s41556-018-0178-0 PubMed 30202049 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-M-puro 8043 bp ds-DNA circular SYN 17-JAN-2024
DEFINITION modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI.
ACCESSION .
VERSION .
KEYWORDS pLVX-M-puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8043)
AUTHORS Zhang Y, Shi J, Liu X, Feng L, Gong Z, Koppula P, Sirohi K, Li X,
Wei Y, Lee H, Zhuang L, Chen G, Xiao ZD, Hung MC, Chen J, Huang P,
Li W, Gan B
TITLE BAP1 links metabolic regulation of ferroptosis to tumour
suppression.
JOURNAL Nat Cell Biol. 2018 Oct;20(10):1181-1192. doi:
10.1038/s41556-018-0178-0. Epub 2018 Sep 10.
PUBMED 30202049
REFERENCE 2 (bases 1 to 8043)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 17, 2024 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8043
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
enhancer 2201..2504
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2505..2708
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2659..2679
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2705..2729
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 2878..3377
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2913..2936)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 3310..3329
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 3398..3997
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3398..3417)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3894..3914
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 4011..4599
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4064..4084)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4482..4493)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4806..5439
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5568..5584)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5568..5584)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5581..5603)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5592..5608
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5616..5646)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5661..5682
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5799..5816)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5970..6558)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6050..6069)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6729..7589)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7352..7371
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7590..7694)
/gene="bla"
/label=AmpR promoter
polyA_signal 7742..7876
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7779..7798)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7833..7852
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
2821 ctcatcggga tcccgctcga ctactcggaa ttccgacatg ctctagataa ttctaccggg
2881 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
2941 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
3001 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
3061 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
3121 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
3181 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
3241 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga agggcctccg
3301 ggggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
3361 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
3421 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
3481 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
3541 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
3601 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
3661 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
3721 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
3781 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
3841 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
3901 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
3961 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc
4021 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
4081 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
4141 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
4201 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
4261 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
4321 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
4381 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
4441 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
4501 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
4561 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
4621 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
4681 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
4741 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
4801 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
4861 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
4921 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
4981 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
5041 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
5101 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
5161 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
5221 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
5281 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
5341 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
5401 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
5461 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
5521 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
5581 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
5641 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
5701 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
5761 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
5821 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
5881 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
5941 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
6001 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
6061 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
6121 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
6181 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
6241 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
6301 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
6361 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
6421 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
6481 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
6541 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
6601 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
6661 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
6721 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
6781 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
6841 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
6901 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
6961 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
7021 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
7081 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
7141 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
7201 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
7261 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
7321 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
7381 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
7441 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
7501 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
7561 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
7621 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
7681 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
7741 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
7801 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
7861 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
7921 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
7981 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
8041 agt
//
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