| 质粒名称: | pLL29 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 15457 |
| 存储实验室: | Chia Lee |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 7029 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 金黄色葡萄球菌 大肠杆菌穿梭载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | 7029 bp |
| 基因突变: | -- |
| GenBank编号 | EF540895 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLL29 was a gift from Chia Lee (Addgene plasmid # 15457 ; http://n2t.net/addgene:15457 ; RRID:Addgene_15457) |
| 参考文献部分: | Improved single-copy integration vectors for Staphylococcus aureus. Luong TT, Lee CY. J Microbiol Methods. 2007 Jul . 70(1):186-90. 10.1016/j.mimet.2007.04.007 PubMed 17512993 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLL29 7029 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLL29
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7029)
AUTHORS Luong TT, Lee CY
TITLE Improved single-copy integration vectors for Staphylococcus aureus.
JOURNAL J Microbiol Methods. 2007 Jul . 70(1):186-90.
PUBMED 17512993
REFERENCE 2 (bases 1 to 7029)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7029
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..57
/label=MCS
/note="pUC18/19 multiple cloning site"
CDS complement(3285..4076)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
CDS complement(5328..6278)
/codon_start=1
/gene="rep101"
/product="RepA protein needed for replication with the
pSC101 origin"
/label=Rep101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 6326..6548
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
ORIGIN
1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttttt
61 tattacctac aacttcttta gcacttaaac gaccatcatc agttattgga acaacgccat
121 aatgcatatg tggtgttttt tcgtccatgt gaactgttgc atataataaa ttatctttac
181 cgtattcttg ttctaaaaac tctttagcat attcaaaaaa ctgctttgta tcttctggcg
241 tttgattatc aaagaaatca ttgtctgatg taattaaacc atcaatgtgt ttaatcgcgt
301 ctgttctaat ttttcttttg cctgtataat tctgttcgat tttttcatca atcaagttat
361 taaaattctg tttattagca tttaccaaat cataatttaa gtaagtttta ctatggtcta
421 tatcttcatt ttcataatta ttattttctc tttgaacatg tttttgtatg cccgttgtat
481 ttgttccaga tttaactttt gaaactctaa caatggaata agacatataa aaatcactcc
541 taagcacaaa ggttttatgt gtatttaata gtttaagtct aacacactag acttattttt
601 aataagtcgt taatacgtgt gctctgcgag gcttaaacct gtttttgcta acgcaaaaaa
661 tgagtggcaa aatgctagcc actcatagtt ctaaaccaaa atataatata actattcaaa
721 ctgcttttca gaacgtttaa atacaataat cgtcaaaaga caacataaaa taataaggat
781 agccatggct acaagaatat tactatacac tccagaagaa taatttataa attccagaac
841 tagtttacga ttaatcaatt gtagtgacaa taaacctcct acaattgcta tacctgttcc
901 ctctgataaa aaacttgtga aatttagcaa actcattcca gaagcaactt cttcttcaga
961 aagactactt gatactattt ttgatataac tgttttagta aaagataatc cgcccataac
1021 aaatataaac ataaaagtag tcaaccacat actaaactca acaaaaaatg caatagttaa
1081 aaaacttata gagatagaca atgatcctaa aataaaaaca aataatgatc cttttctatc
1141 cactaaaaaa ccaccaaaat aaccaaaaac aataacactc atggttccag gaaaaataac
1201 actattacct attgtcgcta catttacatg ataaatagtt ttcatcatat aaggcaccat
1261 tgatataaaa ccagctacta tagaaaatat tagcccacca gaaaacaaac caagcataaa
1321 cggaatgttt ttccctagtt taggattaat aaaagggtta gaaactcttg aaatatgttt
1381 aataaaaatc acaaaaaaga ttgtgaagag tattaaaaaa gtccaattat aatttgtcgt
1441 aaataacata aaacatataa tacttataga cattaaaaca atacctacga tatctaatgt
1501 attttttgtt gatttaccag gtaccattac tttaataaga aaaggtatag ttactattgt
1561 aatcatagga agtataagta ggtaagacca atgaatataa tgtgctatta ttccccctat
1621 tgaaggacct aacccttcac ctaaagctac aattgatcct ataaaaccaa aggctttgcc
1681 ttgttttttt cttgtaatat ttctagctac aaccaccata atcagtgaag ggaatgcagc
1741 agatcctact ccttgtacta acctaccaaa aatcaaaata aaaaagtgat tgtgaccaat
1801 aaaagcaatc aatgaaccaa gacagctcaa actaatacca ataattaaca atttttttat
1861 atttatataa tcagataatt ttccatatac tgctgttcct atcgaaaaag ttaacatata
1921 tgcagtgttt acccagtttg taattccagg agtagtatta aaatgatttg caatatcagg
1981 taaagaaaca tttaaaacca tttcatttaa tacactaaaa aatgaaagaa tacaaagcca
2041 aaataaaacg ctataaaaca aacctttaaa ttttttatat aaactaaaca attttacctc
2101 tttacttgag gtgactaaag tttatagggg taaattattc tttcacctca tataaattcc
2161 ccaaattttt aaatagacac ttcataaaaa aatcctccta aattagaata gtttaaatat
2221 atcatttttg ttcagtaata ttaatatgta cactatttcc aaaatttaaa ttcatgttgc
2281 caaaaatcga ttatgtcttt tgcggggcaa aaaaagggca gattatttga aaaagggcaa
2341 acgcttgtgg aaaagctaaa aggttaaaaa tgacaaaaac cttgatacaa cagtgttttt
2401 ggacgctcgt gtacgttaga gaatgaccgg tttaccatca tacaaggatg ggattaactt
2461 gtgttaaaaa gcctttaata tcagttgtta caaaggattt gtagcgtctt taaaaataaa
2521 aaagggcaga aaaagggcag atacctttta gtacacaagt ttttctaatt tttgctctaa
2581 ctctctgtcc attttctctg ttacatgtgt atacaccttt atagtcgttt tttccagatc
2641 tggaattaat gaggcattct aacattacag aaacaattca aacgtactct catttatata
2701 ctgataaaaa acatcaagct atgagcatat ttgattaaac ggtatcaaat tggtatcaaa
2761 taacaattaa ggagtttata aaatgcgtaa taacaagcct aaaataagta ttcaaaacga
2821 cccatgggaa gtgaaattta tatacattta aatttcatga gacaataaac gttgatttaa
2881 tgcgtttttt tgcctttttt attttcctta ttttttctgt tttacaacaa aatggtatca
2941 aaaatggtat catttgtagt tattttagct tcacatatta aaacaaccac actcctaaat
3001 taataggtgg tgtggttttg ttggttgtgt ggggatcgat agatctggat ctggatttta
3061 atgcggatgt tgcgattact tcgccaacta ttgcgataac aagaaaaagc cagcctttca
3121 tgatatatct cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga
3181 cttgacctga tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta
3241 agccgcgccg cgaagcggcg tcggcttgaa cgaattgtta gacattattt gccgactacc
3301 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
3361 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
3421 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
3481 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
3541 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
3601 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
3661 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
3721 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
3781 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
3841 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
3901 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
3961 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
4021 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg
4081 tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
4141 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
4201 ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
4261 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga
4321 accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
4381 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
4441 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
4501 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
4561 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
4621 catcgtttgt tcgcccagct tctgtatgga acgggcatgc ggatcagtga gggtttgcaa
4681 ctgcgggtca aggatctgga tttcgatcac ggcacgatca tcgtgcggga gggcaagggc
4741 tccaaggatc gggccttgat gttacccgag agcttggcac ccagcctgcg cgagcagggg
4801 aattaattcc cacgggtttt gctgcccgca aacgggctgt tctggtgttg ctagtttgtt
4861 atcagaatcg cagatccggc ttcagccggt ttgccggctg aaagcgctat ttcttccaga
4921 attgccatga ttttttcccc acgggaggcg tcactggctc ccgtgttgtc ggcagctttg
4981 attcgataag cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa
5041 gttgtctcag gtgttcaatt tcatgttcta gttgctttgt tttactggtt tcacctgttc
5101 tattaggtgt tacatgctgt tcatctgtta cattgtcgat ctgttcatgg tgaacagctt
5161 tgaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca ccgttttcat
5221 ctgtgcatat ggacagtttt ccctttgata tgtaacggtg aacagttgtt ctacttttgt
5281 ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg
5341 tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa
5401 ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc
5461 tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttatg taggtaggaa
5521 tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt
5581 tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg
5641 cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt
5701 ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc aagcattaac
5761 atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt
5821 agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca
5881 tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca
5941 ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc
6001 tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt
6061 gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg
6121 ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt
6181 agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc
6241 gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga
6301 ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt
6361 tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct
6421 agaccctctg taaattccgg tagacctttg tgtgtttttt ttgtttatat tcaagtggtt
6481 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg
6541 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt
6601 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg
6661 ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt
6721 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta
6781 caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac
6841 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt
6901 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt
6961 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct
7021 tactgtcgg
//
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