LOCUS Exported 5667 bp ds-DNA circular SYN 28-JUL-2016
DEFINITION Transient expression vector with a GUS reporter gene for plant
transformation.
ACCESSION AF502128
VERSION .
KEYWORDS pBI221
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5667)
AUTHORS Chen PY, Wang CK, Soong SC, To KY.
TITLE Complete sequence of the binary vector pBI121 and its application in
cloning T-DNA insertion from transgenic plants.
JOURNAL Mol. Breed. 11, 287-293 (2003)
REFERENCE 2 (bases 1 to 5667)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, July 28, 2016
FEATURES Location/Qualifiers
source 1..5667
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
misc_feature 1..19
/note="MCS"
promoter 512..857
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 896..2707
/codon_start=1
/gene="uidA (or gusA) from E. coli"
/product="beta-glucuronidase"
/note="GUS"
/translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
terminator 2778..3030
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(3039..3055)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 3529..3633
/gene="bla"
/note="AmpR promoter"
CDS 3634..4494
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4665..5253
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 5577..5607
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 5615..5631
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5639..5655
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 5651..5653
/note="ATG"
ORIGIN
1 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
61 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
121 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
181 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
241 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
301 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
361 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
421 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
481 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
541 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
601 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
661 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
721 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
781 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
841 ttcatttgga gagaacacgg gggactctag aggatccccg ggtggtcagt cccttatgtt
901 acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag
961 tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag
1021 ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa
1081 ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca
1141 gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca
1201 ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt
1261 tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac
1321 tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca
1381 tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac
1441 ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt
1501 tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca
1561 ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg
1621 gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg
1681 tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct
1741 gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg
1801 tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg
1861 ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga
1921 tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg
1981 cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg
2041 ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca
2101 cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg
2161 ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac
2221 ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt
2281 gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga
2341 gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat
2401 caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag
2461 tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc
2521 cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg
2581 cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt
2641 tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa
2701 acaatgaatc aacaactctc ctggcgcacc atcgtcggct acagcctcgg gaattgctac
2761 cgagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
2821 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
2881 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
2941 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
3001 cgcgcgcggt gtcatctatg ttactagatc gggaattcac tggccgtcgt tttacaacgt
3061 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
3121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
3181 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
3241 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
3301 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
3361 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
3421 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
3481 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
3541 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
3601 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
3661 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
3721 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
3781 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
3841 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
3901 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
3961 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
4021 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
4081 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
4141 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
4201 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
4261 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
4321 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
4381 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
4441 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
4501 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
4561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
4621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
4681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
4741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
4801 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
4861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
4921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
4981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
5041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
5101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
5161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
5221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
5281 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
5341 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
5401 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
5461 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
5521 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
5581 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
5641 ggaaacagct atgaccatga ttacgcc
//
