质粒名称: | pBHt2 |
---|---|
出品公司: | 爱迪基因 |
目录编号: | 104175 |
存储实验室: | Seogchan Kang |
载体骨架基本信息 |
|
载体名称: | pCAMBIA1300 |
载体出品公司: | CAMBIA, Canberra, Australia |
载体抗性: | 卡那霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | 在花椰菜花叶病毒35S(CaMV35S)的控制下,用XhoI和bstsxia消化pCAMBIA1300,去除hph基因。将末端钝化并连接到1.4kb的pCB1004 HpaI片段上,该片段在巢曲霉trpC启动子下含有hph基因。然后将hph基因的3′端连接到CaMV35S poly(A)终止信号。 |
载体类型: | 真菌表达 |
筛选标记: | 潮霉素 |
高拷贝/低拷贝: | -- |
生长条件: | 37 ℃ |
插入基因信息 |
|
插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
|
材料和方法部分: | pBHt2 was a gift from Seogchan Kang (Addgene plasmid # 104175 ; http://n2t.net/addgene:104175 ; RRID:Addgene_104175) |
参考文献部分: | Agrobacterium-Mediated Transformation of Fusarium oxysporum: An Efficient Tool for Insertional Mutagenesis and Gene Transfer. Mullins ED, Chen X, Romaine P, Raina R, Geiser DM, Kang S. Phytopathology. 2001 Feb;91(2):173-80. doi: 10.1094/PHYTO.2001.91.2.173. 10.1094/PHYTO.2001.91.2.173 PubMed 18944391 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBHt2 8494 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Empty backbone for Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum. ACCESSION . VERSION . KEYWORDS pBHt2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8494) AUTHORS Mullins ED, Chen X, Romaine P, Raina R, Geiser DM, Kang S TITLE Agrobacterium-Mediated Transformation of Fusarium oxysporum: An Efficient Tool for Insertional Mutagenesis and Gene Transfer. JOURNAL Phytopathology. 2001 Feb;91(2):173-80. doi: 10.1094/PHYTO.2001.91.2.173. PUBMED 18944391 REFERENCE 2 (bases 1 to 8494) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8494 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..25 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" polyA_signal 103..277 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(308..1333) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" protein_bind 1813..1834 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 1849..1879 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1887..1903 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 1892..1914 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 1911..1927 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 1911..1927 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" misc_feature 1936..1992 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(1996..2013) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(1996..2012) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2005..2027) /label=M13/pUC Forward /note="In lacZ gene" misc_feature 2215..2239 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 3540..4169 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 4598..5671 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 5737..5931 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(6167..6189) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 6275..6415 /label=bom /note="basis of mobility region from pBR322" primer_bind 6330..6349 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(6430..6447) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6601..7189) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6681..6700) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7276..8070) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" ORIGIN 1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg 61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact 121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac 181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc 241 cttatctggg aactactcac acattattat ggagaaactc gaaactggtt cccggtcggc 301 atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga 361 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc 421 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat 481 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat 541 acgcccggag gcgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct 601 gctgctccat acaagccaac cacggcctcc agaagaggat gttggcgacc tcgtattggg 661 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca 721 ggacattgtt ggagccgaaa tccgcatgca cgaggtgccg gacttcgggg cagtcctcgg 781 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca 841 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag 901 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg 961 ccgcagcgat cgcatccatg gcctccgcga ccggctggag aacagcgggc agttcggttt 1021 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct 1081 cgctgaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc 1141 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc 1201 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca 1261 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt 1321 caggcttttt catttggatg cttgggtaga ataggtaagt cagattgaat ctgaaataaa 1381 gggaggaagg gcgaacttaa gaaggtatga ccgggtcgtc cacttacctt gcttgacaaa 1441 cgcaccaagt tatcgtgcac caagcagcag atgataataa tgtcctcgtt cctgtctgct 1501 aataagagtc acacttcgag cgccgccgct actgctacaa gtggggctga tctgaccagt 1561 tgcctaaatg aaccatcttg tcaaacgaca caaattttgt gctcaccgcc tggacgacta 1621 aaccaaaata ggcattcatt gttgacctcc actagctcca gccaagccca aaaaatgctc 1681 cttcaatatc agttttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg 1741 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 1801 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 1861 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 1921 ctatgacatg attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag 1981 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt 2041 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga 2101 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag 2161 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag 2221 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa 2281 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc 2341 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg 2401 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca 2461 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta 2521 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct 2581 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact 2641 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg 2701 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag 2761 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga 2821 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg 2881 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga 2941 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc 3001 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac 3061 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga 3121 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc 3181 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg 3241 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag 3301 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat 3361 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct 3421 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt 3481 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca 3541 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc 3601 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc 3661 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca 3721 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga 3781 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg 3841 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc 3901 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc 3961 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc 4021 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg 4081 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg 4141 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag 4201 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc 4261 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca 4321 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga 4381 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag 4441 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc 4501 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct 4561 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg 4621 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca 4681 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc 4741 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag 4801 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg 4861 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg 4921 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg 4981 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct 5041 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg 5101 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga 5161 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa 5221 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta 5281 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg 5341 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta 5401 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg 5461 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga 5521 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta 5581 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct 5641 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc 5701 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa 5761 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg 5821 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta 5881 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg 5941 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg 6001 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg 6061 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc 6121 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 6181 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 6241 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg 6301 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 6361 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 6421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 6481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 6541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 6661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6781 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7021 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7261 catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca 7321 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc 7381 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct 7441 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag 7501 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag 7561 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag 7621 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg 7681 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat 7741 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact 7801 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag 7861 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt 7921 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac 7981 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt 8041 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat 8101 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca 8161 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat 8221 aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt 8281 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct 8341 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg 8401 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc 8461 cgagctgccg gtcggggagc tgttggctgg ctgg //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:pGKO1
- 下一篇:PCS2+Cas9-mSA