| 质粒名称: | p58sheLL |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 37324 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HPV58L1+L2,p58LLw |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p58sheLL was a gift from John Schiller (Addgene plasmid # 37324 ; http://n2t.net/addgene:37324 ; RRID:Addgene_37324) |
| 参考文献部分: | Neutralization of HPV16, 18, 31, and 58 pseudovirions with antisera induced by immunizing rabbits with synthetic peptides representing segments of the HPV16 minor capsid protein L2 surface region. Kondo K, Ishii Y, Ochi H, Matsumoto T, Yoshikawa H, Kanda T. Virology. 2007 Feb 20;358(2):266-72. Epub 2006 Sep 28. 10.1016/j.virol.2006.08.037 PubMed 17010405 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p58sheLL 10730 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p58sheLL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10730)
AUTHORS Kondo K, Ishii Y, Ochi H, Matsumoto T, Yoshikawa H, Kanda T
TITLE Neutralization of HPV16, 18, 31, and 58 pseudovirions with antisera
induced by immunizing rabbits with synthetic peptides representing
segments of the HPV16 minor capsid protein L2 surface region.
JOURNAL Virology. 2007 Feb 20;358(2):266-72. Epub 2006 Sep 28.
PUBMED 17010405
REFERENCE 2 (bases 1 to 10730)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10730
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 3667..4255
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3720..3740)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4138..4149)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4292..4309)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4298..4522
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4568..4996
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4655..4674)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4865..4886
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5005..5025)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS 6954..7322
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
ALRDPAGNCVHFVAEEQD"
polyA_signal 7452..7573
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7489..7508)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7543..7562
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7622..7638)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7622..7638)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7635..7657)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7646..7662
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7670..7700)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7715..7736
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7853..7870)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8024..8609)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8104..8123)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8780..9640)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9403..9422
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9641..9745)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9820..9839)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 10011..10390
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 10391..10594
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 10545..10565
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 10596..10614
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 10617..10635
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 10645..10669
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 tccggagggc cgcgccacca tgagcgtgtg gaggcccagc gaggccaccg tgtacctgcc
61 ccccgtgccc gtgagcaagg tggtgagcac cgacgagtac gtgagcagga ccagcatcta
121 ctactacgcc ggcagcagca ggctgctggc cgtgggcaac ccctacttca gcatcaagag
181 ccccaacaac aacaagaagg tgctggtgcc caaggtgagc ggcctgcagt acagggtgtt
241 cagggtgagg ctgcccgacc ccaacaagtt cggcttcccc gacaccagct tctacaaccc
301 cgacacccag aggctggtgt gggcctgcgt gggcctggag atcggcaggg gccagcccct
361 gggcgtgggc gtgagcggcc acccctacct gaacaagttc gacgacaccg agaccagcaa
421 caggtacccc gcccagcccg gcagcgacaa cagggagtgc ctgagcatgg actacaagca
481 gacccagctg tgcctgatcg gctgcaagcc ccccaccggc gagcactggg gcaagggcgt
541 ggcctgcaac aacaacgccg ccgccaccga ctgccccccc ctggagctgt tcaacagcat
601 catcgaggac ggcgacatgg tggacaccgg cttcggctgc atggacttcg gcaccctgca
661 ggccaacaag agcgacgtgc ccatcgacat ctgcaacagc acctgcaagt accccgacta
721 cctgaagatg gccagcgagc cctacggcga cagcctgttc ttcttcctga ggagggagca
781 gatgttcgtg aggcacttct tcaacagggc cggcaagctg ggcgaggccg tgcccgacga
841 cctgtacatc aagggcagcg gcaacaccgc cgtgatccag agcagcgcct tcttccccac
901 ccccagcggc agcatcgtga ccagcgagag ccagctgttc aacaagccct actggctgca
961 gagggcccag ggccacaaca acggcatctg ctggggcaac cagctgttcg tgaccgtggt
1021 ggacaccacc aggagcacca acatgaccct gtgcaccgag gtgaccaagg agggcaccta
1081 caagaacgac aacttcaagg agtacgtgag gcacgtggag gagtacgacc tgcagttcgt
1141 gttccagctg tgcaagatca ccctgaccgc cgagatcatg acctacatcc acaccatgga
1201 cagcaacatc ctggaggact ggcagttcgg cctgaccccc ccccccagcg ccagcctgca
1261 ggacacctac aggttcgtga ccagccaggc catcacctgc cagaagaccg ccccccccaa
1321 ggagaaggag gaccccctga acaagtacac cttctgggag gtgaacctga aggagaagtt
1381 cagcgccgac ctggaccagt tccccctggg caggaagttc ctgctgcaga gcggcctgaa
1441 ggccaagccc aggctgaaga ggagcgcccc caccaccagg gcccccagca ccaagaggaa
1501 gaaggtgaag aagtgaaagc tttttgaatt ctttggatcc actagggctc gaggaagctt
1561 aaaacagctc tggggttgta cccaccccag aggcccacgt ggcggctagt actccggtat
1621 tgcggtaccc ttgtacgcct gttttatact cccttcccgt aacttagacg cacaaaacca
1681 agttcaatag aagggggtac aaaccagtac caccacgaac aagcacttct gtttccccgg
1741 tgatgtcgta tagactgctt gcgtggttga aagcgacgga tccgttatcc gcttatgtac
1801 ttcgagaagc ccagtaccac ctcggaatct tcgatgcgtt gcgctcagca ctcaacccca
1861 gagtgtagct taggctgatg agtctggaca tccctcaccg gtgacggtgg tccaggctgc
1921 gttggcggcc tacctatggc taacgccatg ggacgctagt tgtgaacaag gtgtgaagag
1981 cctattgagc tacataagaa tcctccggcc cctgaatgcg gctaatccca acctcggagc
2041 aggtggtcac aaaccagtga ttggcctgtc gtaacgcgca agtccgtggc ggaaccgact
2101 actttgggtg tccgtgtttc cttttatttt attgtggctg cttatggtga caatcacaga
2161 ttgttatcat aaagcgaatt ggattgcggc cgcgccacca tgaggcacaa gaggagcacc
2221 aggaggaaga gggccagcgc cacccagctg taccagacct gcaaggccag cggcacctgc
2281 ccccccgacg tgatccccaa ggtggagggc accaccatcg ccgaccagat cctgaggtac
2341 ggcagcctgg gcgtgttctt cggcggcctg ggcatcggca ccggcagcgg caccggcggc
2401 aggaccggct acgtgcccct gggcagcacc ccccccagcg aggccatccc cctgcagccc
2461 atcaggcccc ccgtgaccgt ggacaccgtg ggccccctgg acagcagcat cgtgagcctg
2521 atcgaggaga gcagcttcat cgacgccggc gcccccgccc ccagcatccc cacccccagc
2581 ggcttcgaca tcaccaccag cgccgacacc acccccgcca tcctgaacgt gagcagcatc
2641 ggcgagagca gcatccagac cgtgagcacc cacctgaacc ccagcttcac cgagcccagc
2701 gtgctgaggc cccccgcccc cgccgaggcc agcggccacc tgatcttcag cagccccacc
2761 gtgagcaccc acagctacga gaacatcccc atggacacct tcgtgatcag caccgacagc
2821 ggcaacgtga ccagcagcac ccccatcccc ggcagcaggc ccgtggccag gctgggcctg
2881 tacagcagga acacccagca ggtgaaggtg gtggaccccg ccttcctgac cagcccccac
2941 aggctggtga cctacgacaa ccccgccttc gagggcttca accccgagga caccctgcag
3001 ttccagcaca gcgacatcag ccccgccccc gaccccgact tcctggacat cgtggccctg
3061 cacaggcccg ccctgaccag caggaggggc accgtgaggt acagcagggt gggccagaag
3121 gccaccctga ggaccaggag cggcaagcag atcggcgcca aggtgcacta ctaccaggac
3181 ctgagcccca tccagcccgt gcaggagcag gtgcagcagc agcagcagtt cgagctgcag
3241 agcctgaaca ccagcgtgag cccctacagc atcaacgacg gcctgtacga catctacgcc
3301 gacgacgccg acaccatcca cgacttccag agccccctgc acagccacac cagcttcgcc
3361 accaccagga ccagcaacgt gagcatcccc ctgaacaccg gcttcgacac ccccctggtg
3421 agcctggagc ccggccccga catcgccagc agcgtgacca gcatgagcag ccccttcatc
3481 cccatcagcc ccctgacccc cttcaacacc atcatcgtgg acggcgccga cttcatgctg
3541 caccccagct acttcatcct gaggaggagg aggaagaggt tcccctactt cttcgccgac
3601 gtgagggtgg ccgcctgaaa gctttttgaa ttctttggat ccactagcga tatcaagctt
3661 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
3721 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
3781 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
3841 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
3901 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
3961 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
4021 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
4081 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
4141 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
4201 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
4261 taccgtcggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
4321 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
4381 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
4441 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
4501 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
4561 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
4621 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
4681 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
4741 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
4801 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
4861 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
4921 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
4981 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
5041 gctccccagc aggcagaagt atgcaaagca tgcagaattc tatcaaatat ttaaagaaaa
5101 aaaaattgta tcaactttct acaatctctt tcagaagaca gaagcagagg gaatacttcc
5161 taaatcattc aactaggcca gcattacctt aataccggaa ctagaaaatg acattacaag
5221 aaaagaaaac aacagaccaa tatctctcat gaacaaagat acaaacattt tcaacaaaat
5281 attagcaaaa agaatccaag aatgtatcaa aaaatataca ccacaaccaa gtagaattta
5341 ttccagatat gtaagggtgg ttcaacgttt gaaaatcaat taacgtaatt tgtcccatca
5401 acaggttaaa gaagaaaatc acatggtcat attgatagac acagaaaaag catttgacaa
5461 aatttaacac ccattcatga tgcaatctct cagtaaacta ggaatagagg aaaacttcct
5521 cagcttgaat gtaccttcct ctcaattttg ctatgaacct gaaactcctc ttaaaaaata
5581 aagtttttca tttaaaaaga aaacaaaaaa catggaggag cgttgatgta tctcatttta
5641 gaccaatcag ctatggatag ttaggcgaca gcacagatag ctgctgtact tctgtttctg
5701 gcaatgttcc agactacatt taaaaaattt ttaattatag acttgtactt aatgttcaag
5761 aaaaatatga aaatggcttt gccgtgttaa tgctactctt ttttaaaaaa aactaaagtt
5821 caaactttat ttatatttca ttagtttttt agctactgtt ctttttctgt tctgggatct
5881 cattcagaat gccacattac atataattct catgtctcct tgggttcctc ttagttttga
5941 cagttcctca gacttttctt atttttgatg accttgacag ttttgaggag tactggttag
6001 atatagggta atggttttta aagtatattt gtcatgattt atactggggt aagggtttgg
6061 ggaggaagcc catggggtaa agtactgttc tcatcacatc atatcaaggt tatataccat
6121 caatattgcc acagatgtta cttagccttt taatatttct ctaatttagt gtatatgcaa
6181 tgatagttct ctgatttctg agattgagtt tctcatgtgt aatgattatt tagagtttct
6241 ctttcatctg ttcaaatttt tgtctagttt tattttttac tgatttgtaa gacttctttt
6301 tataatctgc atattacaat tctctttact ggggtgttgc aaatattttc tgtcattcta
6361 tggcctgact tttcttaatg gttttttaat tttaaaaata agtcttaata ttcatgcaat
6421 ctaattaaca atcttttctt tgtggttagg actttgagtc ataagaaatt tttctctaca
6481 ctgaagtcat gatggcatgc ttctatatta ttttctaaaa gatttaaagt tttgccttct
6541 ccatttagac ttataattca ctggaatttt tttgtgtgta tggtatgaca tatgggttcc
6601 cttttatttt ttacatataa atatatttcc ctgtttttct aaaaaagaaa aagatcatca
6661 ttttcccatt gtaaaatgcc atattttttt cataggtcac ttacatatat caatgggtct
6721 gtttctgagc tctactctat tttatcagcc tcactgtcta tccccacaca tctcatgctt
6781 tgctctaaat cttgatattt agtggaacat tctttcccat tttgttctac aagaatattt
6841 ttgttattgt cttttgggct tctatataca ttttagaatg aggttggcaa gttaacaaac
6901 agcttttttg gggtgaacat attgactaca aatttatgtg gaaagaaagt accaagttga
6961 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
7021 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
7081 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
7141 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
7201 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
7261 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
7321 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
7381 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
7441 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
7501 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
7561 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
7621 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
7681 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
7741 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
7801 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
7861 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
7921 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
7981 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
8041 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
8101 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
8161 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
8221 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
8281 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
8341 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
8401 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
8461 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
8521 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
8581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
8641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
8701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
8761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
8821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
8881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
8941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
9001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
9061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
9121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
9181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
9241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
9301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
9361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
9421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
9481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
9541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
9601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
9661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
9721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
9781 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
9841 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
9901 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
9961 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
10021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
10081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
10141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
10201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
10261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
10321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
10381 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
10441 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggaaccaaaa
10501 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
10561 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
10621 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
10681 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
//
