买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
Plasmid is part of the " DEL-MARKER-SET ". For an individual plasmid order use accession number P30114.
Transformed E.coli cells are resistant to ampicillin and kanamycin. The plasmid contains the kanMX gene from Tn903 that confers resistance to the aminoglycosid antibiotic G418 of transformed yeasts. Due to the flanking loxP sites the deletion marker can be removed by expressing cre recombinase.
Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants.
载体序列
LOCUS AF298793 4009 bp DNA circular SYN 11-MAR-2002
DEFINITION PCR template vector pUG6, complete sequence.
ACCESSION AF298793
VERSION AF298793.1 GI:11344909
KEYWORDS .
SOURCE PCR template vector pUG6
ORGANISM PCR template vector pUG6
artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4009)
AUTHORS Guldener,U., Heck,S., Fielder,T., Beinhauer,J. and Hegemann,J.H.
TITLE A new efficient gene disruption cassette for repeated use in
budding yeast
JOURNAL Nucleic Acids Res. 24 (13), 2519-2524 (1996)
MEDLINE 96292254
PUBMED 8692690
REFERENCE 2 (bases 1 to 4009)
AUTHORS Gueldener,U., Heinisch,J., Koehler,G.J., Voss,D. and Hegemann,J.H.
TITLE A second set of loxP marker cassettes for Cre-mediated multiple
gene knockouts in budding yeast
JOURNAL Nucleic Acids Res. 30 (6), E23 (2002)
MEDLINE 21882009
PUBMED 11884642
REFERENCE 3 (bases 1 to 4009)
AUTHORS Gueldener,U., Hegemann,J.H., Heck,S., Fiedler,T. and Beinhauer,J.D.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) Institut fuer Mikrobiologie,
Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
40225, Germany
FEATURES Location/Qualifiers
source 1..4009
/organism="PCR template vector pUG6"
/mol_type="other DNA"
/db_xref="taxon:142962"
/note="derived from Saccharomyces cerevisiae"
misc_feature 53..86
/note="loxP site"
promoter 87..484
/note="from TEF"
gene 485..1294
/gene="kanMX"
CDS 485..1294
/gene="kanMX"
/codon_start=1
/transl_table=11
/product="geneticin resistance protein"
/protein_id="AAG34543.1"
/db_xref="GI:11344910"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
terminator 1295..1559
/note="from TEF"
misc_feature 1560..1593
/note="loxP site"
gene complement(2682..3542)
/gene="AmpR"
CDS complement(2682..3542)
/gene="AmpR"
/codon_start=1
/transl_table=11
/product="ampicillin resistance protein"
/protein_id="AAG34544.1"
/db_xref="GI:11344911"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1026 a 953 c 949 g 1081 t
ORIGIN
1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acaaccctta atataacttc
61 gtataatgta tgctatacga agttattagg tctagagatc tgtttagctt gcctcgtccc
121 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg
181 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg
241 tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct
301 cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc
361 acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc
421 atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac
481 aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga ttaaattcca acatggatgc
541 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
601 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
661 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
721 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
781 ccccggcaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
841 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
901 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
961 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
1021 aatgcataag cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
1081 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
1141 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
1201 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
1261 gcagtttcat ttgatgctcg atgagttttt ctaatcagta ctgacaataa aaagattctt
1321 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca
1381 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta
1441 agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg
1501 tcgattcgat actaacgccg ccatccagtg tcgaaaacga gctctcgaga acccttaata
1561 taacttcgta taatgtatgc tatacgaagt tattaggtga tatcagatcc actagtggcc
1621 tatgcggccg cggatctgcc ggtctcccta tagtgagtcg tattaatttc gataagccag
1681 gttaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
1741 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
1801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
1861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
1921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
1981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
2041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
2101 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
2161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
2221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
2281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
2341 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
2401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
2461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
2521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
2581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
2641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
2701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
2761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
2821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
2881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
2941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
3001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
3061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
3121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
3181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
3241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
3301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
3361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
3421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
3481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
3541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
3601 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
3661 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
3721 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
3781 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
3841 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
3901 gagcagattg tactgagagt gcaccatatg gacatattgt cgttagaacg cggctacaat
3961 taatacataa ccttatgtat catacacata cgatttaggt gacactata
//
