出品人: | Lynch,M.D. and Gill,R.T. |
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载体名称: | pBT-2载体,pBT2载体,pBT2质粒,pBT-2质粒 |
质粒类型: | 广宿主载体;克隆载体;原核载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | 无 |
载体大小: | 2558 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 卡那霉素 |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 多个菌属 |
备注: | -- |
产品目录号: | -- |
组成型/诱导型: | 组成型 |
买家导航
pBT-2载体 pBT2质粒基本信息
pBT-2质粒图谱 pBT2载体图谱和pBT-2载体序列 pBT2质粒序列多克隆位点信息
pBT-2质粒 pBT2载体简介
pBT-2质粒序列 pBT2载体序列
LOCUS DQ058720 2558 bp DNA circular SYN 08-MAY-2006 DEFINITION Broad host range vector pBT-2, complete sequence. ACCESSION DQ058720 VERSION DQ058720.1 GI:69048110 KEYWORDS . SOURCE Broad host range vector pBT-2 ORGANISM Broad host range vector pBT-2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2558) AUTHORS Lynch,M.D. and Gill,R.T. TITLE Broad host range vectors for stable genomic library construction PUBMED 16496398 REFERENCE 2 (bases 1 to 2558) AUTHORS Lynch,M.D. and Gill,R.T. TITLE Direct Submission JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering, University of Colorado, ECCH 111, Campus Box 424, Boulder, CO 80309, USA FEATURES Location/Qualifiers source 1..2558 /organism="Broad host range vector pBT-2" /mol_type="other DNA" /db_xref="taxon:334952" regulatory 60..96 /regulatory_class="terminator" /note="SoxR terminator" gene 872..1534 /gene="rep" /note="rep gene from pBBR1, GenBank Accession Number X66730" CDS 872..1534 /gene="rep" /note="required for replication" /codon_start=1 /transl_table=11 /product="Rep" /protein_id="AAY99689.1" /db_xref="GI:69048111" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP RR" gene 1631..2446 /gene="kan" /note="kanamycin resistance gene from TN903" CDS 1631..2446 /gene="kan" /codon_start=1 /transl_table=11 /product="kanamycin kinase" /protein_id="AAY99690.1" /db_xref="GI:69048112" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGL VGASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" regulatory 2463..2494 /regulatory_class="terminator" /note="TonB terminator" ORIGIN 1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg 121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca 181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc 241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag 301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc 361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc 421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg 481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt 541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg 601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg 661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac 721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg 781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa 841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat 901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg 961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca 1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg 1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt 1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg 1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca 1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta 1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat 1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg 1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga 1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca 1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa 1621 ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc 1681 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg 1741 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg 1801 caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact ggctgacgga 1861 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact 1921 caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg 1981 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg 2041 taattgtcct tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa 2101 taacggtttg gttggtgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca 2161 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg 2221 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt 2281 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg 2341 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga 2401 tatgaataaa ttgcagtttc acttgatgct cgatgagttt ttctgagggc ggatccccct 2461 caagtcaaaa gcctccggtc ggaggctttt gactttctgc tatggaggtc aggtatgatt 2521 taaatggtca gtattgagcg atatctagag aattcgtc //