| 质粒名称: | pCXLE-hOCT3/4 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 27076 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pCXLE |
| 载体出品公司: | -- |
| 空载体大小: | 10180 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | OCT3/4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1108 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI (未破坏) |
| 3’克隆位点: | EcoRI (未破坏) |
| 5’测序引物 | pCAG-F |
| 3’测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCXLE-hOCT3/4 was a gift from Shinya Yamanaka (Addgene plasmid # 27076 ; http://n2t.net/addgene:27076 ; RRID:Addgene_27076) |
| 参考文献部分: | A more efficient method to generate integration-free human iPS cells. Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S. Nat Methods. 2011 May;8(5):409-12 10.1038/nmeth.1591 PubMed 21460823 |
买家导航
pCXLEhOCT3/4载体基本信息
pCXLEhOCT3/4载体图谱质粒图谱和多克隆位点信息
pCXLEhOCT3/4载体简介
pCXLEhOCT3/4载体序列
LOCUS pCXLE-hOCT3/4 11288 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Non-integrating (episomal) expression of human OCT3/4.
ACCESSION .
VERSION .
KEYWORDS pCXLE-hOCT3/4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11288)
AUTHORS Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong
H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
M, Takahashi J, Saji H, Yamanaka S
TITLE A more efficient method to generate integration-free human iPS
cells.
JOURNAL Nat Methods. 2011 Apr 3. ():.
PUBMED 21460823
REFERENCE 2 (bases 1 to 11288)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11288
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 31..619
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(84..104)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(502..513)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(709..728)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 774..829
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(828..847)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(1190..1206)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1190..1206)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1203..1225)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1214..1230
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1238..1268)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1283..1304
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
protein_bind complement(1393..1426)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 1887..3812
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
protein_bind complement(3980..4013)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin 4200..5989
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind complement(6531..6548)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6702..7290)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6782..6801)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7461..8321)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8084..8103
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8322..8426)
/gene="bla"
/label=AmpR promoter
enhancer 8457..8836
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8838..9114
/label=chicken beta-actin promoter
intron 9115..10131
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 10055..10077
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 10139..10158
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
ORIGIN
1 aattcaagct tcggggacta gtcatatgat aatcaacctc tggattacaa aatttgtgaa
61 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
121 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
181 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
241 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
301 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
361 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
421 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
481 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
541 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
601 ctttgggccg cctccccgca tcggtaaatt cactcctcag gtgcaggctg cctatcagaa
661 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
721 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
781 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
841 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
901 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
961 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
1021 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
1081 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
1141 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
1201 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
1261 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
1321 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ctcaattccg atcatattca
1381 ataaccctta atataacttc gtataatgta tgctatacga agttattagg tctgaagagg
1441 agtttacgtc cagccaagct taggatcaat tctcatgttt gacagcttat catcgataag
1501 ctgatcctca caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg
1561 tgaccttgaa gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa
1621 gggggacgag gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca
1681 gcgggagttt tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg
1741 ggaattttca gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc
1801 tgttggaggg ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg
1861 tctattgatc tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa
1921 atggcctagg agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc
1981 aaagaagagg gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg
2041 gaagaccagg agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga
2101 gaccccaaaa acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag
2161 gagcaggagc gggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagcag
2221 gaggaggggc aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag
2281 caggaggagg ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag
2341 gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag
2401 gaggaggggc aggagcagga ggaggggcag gaggggcagg agcaggagga ggggcaggag
2461 gggcaggagg ggcaggagca ggaggagggg caggagcagg aggggcagga ggggcaggag
2521 gggcaggagc aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag
2581 caggaggggc aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag
2641 gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag
2701 gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggagggg
2761 caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag
2821 gagcaggagg aggggcagga gcaggagggg caggagcagg aggtggaggc cggggtcgag
2881 gaggcagtgg aggccggggt cgaggaggta gtggaggccg gggtcgagga ggtagtggag
2941 gccgccgggg tagaggacgt gaaagagcca gggggggaag tcgtgaaaga gccaggggga
3001 gaggtcgtgg acgtggagaa aagaggccca ggagtcccag tagtcagtca tcatcatccg
3061 ggtctccacc gcgcaggccc cctccaggta gaaggccatt tttccaccct gtaggggaag
3121 ccgattattt tgaataccac caagaaggtg gcccagatgg tgagcctgac gtgcccccgg
3181 gagcgataga gcagggcccc gcagatgacc caggagaagg cccaagcact ggaccccggg
3241 gtcagggtga tggaggcagg cgcaaaaaag gagggtggtt tggaaagcat cgtggtcaag
3301 gaggttccaa cccgaaattt gagaacattg cagaaggttt aagagctctc ctggctagga
3361 gtcacgtaga aaggactacc gacgaaggaa cttgggtcgc cggtgtgttc gtatatggag
3421 gtagtaagac ctccctttac aacctaaggc gaggaactgc ccttgctatt ccacaatgtc
3481 gtcttacacc attgagtcgt ctcccctttg gaatggcccc tggacccggc ccacaacctg
3541 gcccgctaag ggagtccatt gtctgttatt tcatggtctt tttacaaact catatatttg
3601 ctgaggtttt gaaggatgcg attaaggacc ttgttatgac aaagcccgct cctacctgca
3661 atatcagggt gactgtgtgc agctttgacg atggagtaga tttgcctccc tggtttccac
3721 ctatggtgga aggggctgcc gcggagggtg atgacggaga tgacggagat gaaggaggtg
3781 atggagatga gggtgaggaa gggcaggagt gatgtaactt gttaggagac gccctcaatc
3841 gtattaaaag ccgtgtattc ccccgcacta aagaataaat ccccagtaga catcatgcgt
3901 gctgttggtg tatttctggc catctgtctt gtcaccattt tcgtcctccc aacatggggc
3961 aattgccgga acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtcc
4021 ctcgaagagg ttcactagcg gatctcaatt gggcataccc atgttgtcac gtcactcagc
4081 tccgcgctca acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc
4141 agacatgcga cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc
4201 aggaaaagga caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg
4261 atagcactcc cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca
4321 tatgctaccc ggatacagat taggatagca tatactaccc agatatagat taggatagca
4381 tatgctaccc agatatagat taggatagcc tatgctaccc agatataaat taggatagca
4441 tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
4501 tatgctaccc agatatagat taggatagca tatgctaccc agatatagat taggatagca
4561 tatgctatcc agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata
4621 ctaccctaat ctctattagg atagcatatg ctacccggat acagattagg atagcatata
4681 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
4741 ctacccagat ataaattagg atagcatata ctacccagat atagattagg atagcatatg
4801 ctacccagat atagattagg atagcctatg ctacccagat atagattagg atagcatatg
4861 ctatccagat atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca
4921 gcgacctcgt gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg
4981 taatttgtcc tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg
5041 caggtattcc ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt
5101 agcggggtta caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc
5161 ggcgtgtggg ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt
5221 gtctttgttt atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga
5281 caaccagtgg agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt
5341 gtaacaacat ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta
5401 tcatattgca ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc
5461 agtctttacg gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt
5521 cattcctaca ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc
5581 acaatgccac cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt
5641 gttttcgagc acctcacata caccttactg ttcacaactc agcagttatt ctattagcta
5701 aacgaaggag aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat
5761 cttcagccac tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat
5821 gtaaataaaa ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt
5881 actaacccta attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg
5941 ttagtctgga tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca
6001 agggggcctt ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac
6061 acaggcccct ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg
6121 tacatgtccc ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt
6181 tgtgttgcag tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca
6241 aatgtgcaca tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt
6301 ccccccccgt gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag
6361 ggattacatg cactgccccg cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt
6421 ccggggcggg gcatcgatcc tctagagtcg acgctagcgg atccgctgca ttaatgaatc
6481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
6541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
6601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
6661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
6721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
6781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
6841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
6901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
6961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
7021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
7081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
7141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
7201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
7261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
7321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
7381 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
7441 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
7501 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
7561 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
7621 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
7681 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
7741 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
7801 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
7861 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
7921 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
7981 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
8041 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
8101 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
8161 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
8221 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
8281 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
8341 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
8401 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
8461 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
8521 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
8581 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
8641 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
8701 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
8761 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
8821 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
8881 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
8941 ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
9001 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
9061 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
9121 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
9181 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
9241 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
9301 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
9361 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
9421 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
9481 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
9541 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
9601 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
9661 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
9721 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
9781 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
9841 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
9901 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
9961 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
10021 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
10081 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
10141 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcgccct tcaccatggc
10201 gggacacctg gcttcggatt tcgccttctc gccccctcca ggtggtggag gtgatgggcc
10261 aggggggccg gagccgggct gggttgatcc tcggacctgg ctaagcttcc aaggccctcc
10321 tggagggcca ggaatcgggc cgggggttgg gccaggctct gaggtgtggg ggattccccc
10381 atgccccccg ccgtatgagt tctgtggggg gatggcgtac tgtgggcccc aggttggagt
10441 ggggctagtg ccccaaggcg gcttggagac ctctcagcct gagggcgaag caggagtcgg
10501 ggtggagagc aactccgatg gggcctcccc ggagccctgc accgtcaccc ctggtgccgt
10561 gaagctggag aaggagaagc tggagcaaaa cccggaggag tcccaggaca tcaaagctct
10621 gcagaaagaa ctcgagcaat ttgccaagct cctgaagcag aagaggatca ccctgggata
10681 tacacaggcc gatgtggggc tcaccctggg ggttctattt gggaaggtat tcagccaaac
10741 gaccatctgc cgctttgagg ctctgcagct tagcttcaag aacatgtgta agctgcggcc
10801 cttgctgcag aagtgggtgg aggaagctga caacaatgaa aatcttcagg agatatgcaa
10861 agcagaaacc ctcgtgcagg cccgaaagag aaagcgaacc agtatcgaga accgagtgag
10921 aggcaacctg gagaatttgt tcctgcagtg cccgaaaccc acactgcagc agatcagcca
10981 catcgcccag cagcttgggc tcgagaagga tgtggtccga gtgtggttct gtaaccggcg
11041 ccagaagggc aagcgatcaa gcagcgacta tgcacaacga gaggattttg aggctgctgg
11101 gtctcctttc tcagggggac cagtgtcctt tcctctggcc ccagggcccc attttggtac
11161 cccaggctat gggagccctc acttcactgc actgtactcc tcggtccctt tccctgaggg
11221 ggaagccttt ccccctgtct ctgtcaccac tctgggctct cccatgcatt caaactgagg
11281 taagggcg
//
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