| 质粒名称: | CoV2-Spike-D614G |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 177960 |
| 存储实验室: | Jennifer Doudna |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) |
| 物种来源: | Other |
| 插入大小: | -- |
| 基因突变: | D614G |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | CoV2-Spike-D614G was a gift from Jennifer Doudna (Addgene plasmid # 177960 ; http://n2t.net/addgene:177960 ; RRID:Addgene_177960) |
| 参考文献部分: | Rapid assessment of SARS-CoV-2 evolved variants using virus-like particles. Syed AM, Taha TY, Tabata T, Chen IP, Ciling A, Khalid MM, Sreekumar B, Chen PY, Hayashi JM, Soczek KM, Ott M, Doudna JA. Science. 2021 Nov 4:eabl6184. doi: 10.1126/science.abl6184. 10.1126/science.abl6184 PubMed 34735219 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS CoV2-Spike-D614G 9169 bp ds-DNA circular SYN 17-NOV-2021
DEFINITION Transient mammalian expression of codon optimized SARS-CoV-2 Spike
protein (original Wuhan Hu1 sequence + D614G mutation).
ACCESSION .
VERSION .
KEYWORDS CoV2-Spike-D614G
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9169)
AUTHORS Syed AM, Taha TY, Tabata T, Chen IP, Ciling A, Khalid MM, Sreekumar
B, Chen PY, Hayashi JM, Soczek KM, Ott M, Doudna JA
TITLE Rapid assessment of SARS-CoV-2 evolved variants using virus-like
particles.
JOURNAL Science. 2021 Nov 4:eabl6184. doi: 10.1126/science.abl6184.
PUBMED 34735219
REFERENCE 2 (bases 1 to 9169)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 17, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9169
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(529..1389)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1152..1171
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1390..1494)
/gene="bla"
/label=AmpR promoter
primer_bind complement(1569..1588)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 1760..2139
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2140..2343
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2294..2314
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2388..2407
/label=T7
/note="T7 promoter, forward primer"
promoter 2388..2406
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6285..6302)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6291..6515
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6561..6989
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6648..6667)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6858..6879
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6998..7018)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7003..7332
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7183..7318
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7245..7264
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7399..8193
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7453..7472)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 8063..8082
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 8367..8488
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8404..8423)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8458..8477
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8537..8553)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8537..8553)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8550..8572)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8561..8577
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8585..8615)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8630..8651
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8768..8785)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8939..358)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9019..9038)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
61 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
361 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
421 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
1021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
1081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
1141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
1201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
1261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
1321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
1381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
1441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
1501 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
1561 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
1621 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
1681 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
1741 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
1801 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
1861 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
1921 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
1981 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
2041 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
2101 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
2161 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
2221 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
2281 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
2341 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
2401 tatagggaga cccaagctgg ctagcatgtt tgtcttcctg gtcctgctgc ctctggtctc
2461 gtctcagtgc gtgaacctga ctactagaac ccagctgcct cctgcctata ctaactcctt
2521 cacccgcggc gtgtactacc cagacaaggt gttccgcagc tccgtgctgc actccaccca
2581 ggatctgttc ctgcccttct tcagcaacgt gacctggttc cacgccatcc acgtgagcgg
2641 caccaatggc accaagcggt tcgacaatcc cgtgctgcca ttcaacgatg gcgtgtactt
2701 cgcctccacc gagaagagca acatcatccg cggctggatc ttcggcacca ccctggactc
2761 caagacccag agcctgctga tcgtgaacaa tgccaccaac gtggtcatca aggtgtgcga
2821 gttccagttc tgcaatgatc cattcctggg cgtgtactac cacaagaaca ataagtcctg
2881 gatggagagc gagttccgcg tgtacagctc cgccaacaat tgcaccttcg agtacgtgtc
2941 ccagcccttc ctgatggacc tggagggcaa gcagggcaat ttcaagaacc tgcgcgagtt
3001 cgtgttcaag aatatcgatg gctacttcaa gatctactcc aagcacaccc ccatcaacct
3061 ggtgcgcgac ctgccacagg gcttcagcgc cctggagcca ctggtggatc tgccaatcgg
3121 catcaacatc accaggttcc agaccctgct ggccctgcac cgcagctacc tgaccccagg
3181 cgacagctcc agcggatgga ccgctggagc tgctgcctac tacgtgggct acctgcagcc
3241 ccgcaccttc ctgctgaagt acaacgagaa tggcaccatc accgacgccg tggattgcgc
3301 cctggatcca ctgtccgaga caaagtgcac cctgaagagc ttcaccgtgg agaagggcat
3361 ctaccagacc tccaatttcc gcgtgcagcc aaccgagagc atcgtgcgct tccccaatat
3421 caccaacctg tgcccattcg gcgaggtgtt caacgctacc aggttcgcca gcgtgtacgc
3481 ttggaatcgc aagcgcatct ccaactgcgt ggccgactac agcgtgctgt acaactccgc
3541 cagcttctcc accttcaagt gctacggcgt gtcccccacc aagctgaatg atctgtgctt
3601 caccaacgtg tacgccgata gcttcgtgat caggggcgac gaggtgcgcc agatcgctcc
3661 aggacagacc ggcaagatcg ctgactacaa ttacaagctg cccgacgatt tcaccggctg
3721 cgtgatcgcc tggaactcca acaatctgga tagcaaagtg ggcggcaact acaattacct
3781 gtaccgcctg ttccgcaagt ccaatctgaa gccattcgag cgcgacatct ccaccgagat
3841 ctaccaggct ggaagcaccc catgcaatgg agtggagggc ttcaactgct acttccccct
3901 gcagagctac ggcttccagc caaccaacgg agtgggatac cagccataca gggtggtggt
3961 gctgtccttc gagctgctgc acgctccagc taccgtgtgc ggaccaaaga agagcaccaa
4021 tctggtgaag aacaagtgcg tgaacttcaa tttcaacggc ctgaccggaa ccggcgtgct
4081 gaccgagtcc aacaagaagt tcctgccatt ccagcagttc ggaagggaca tcgctgatac
4141 caccgacgcc gtgcgcgacc cacagaccct ggagatcctg gatatcaccc catgctcctt
4201 cggcggcgtg agcgtgatca ccccaggaac caataccagc aaccaggtgg ccgtgctgta
4261 ccagggcgtg aattgcaccg aggtgccagt ggctatccac gctgatcagc tgaccccaac
4321 ctggcgcgtg tacagcaccg gatccaacgt gttccagacc cgcgccggat gcctgatcgg
4381 agctgagcac gtgaacaatt cctacgagtg cgacatccca atcggagctg gaatctgcgc
4441 cagctaccag acccagacca actccccaag gagggctcgc agcgtggcca gccagtccat
4501 catcgcctac accatgtccc tgggcgccga gaatagcgtg gcctacagca acaattccat
4561 cgccatccca accaacttca ccatctccgt gaccaccgag atcctgcccg tgtccatgac
4621 caagaccagc gtggactgca ccatgtacat ctgcggcgat tccaccgagt gcagcaacct
4681 gctgctgcag tacggcagct tctgcaccca gctgaatcgc gccctgaccg gaatcgctgt
4741 ggagcaggat aagaacaccc aggaggtgtt cgcccaggtg aagcagatct acaagacccc
4801 cccaatcaag gacttcggcg gcttcaattt cagccagatc ctgcccgatc caagcaagcc
4861 ctccaagcgc agcttcatcg aggacctgct gttcaacaag gtgaccctgg ccgatgccgg
4921 cttcatcaag cagtacggcg attgcctggg cgacatcgct gcccgcgacc tgatctgcgc
4981 ccagaagttc aatggcctga ccgtgctgcc accactgctg accgatgaga tgatcgctca
5041 gtacacctcc gccctgctgg ccggaaccat caccagcgga tggaccttcg gcgctggagc
5101 cgccctgcag atccccttcg ccatgcagat ggcctaccgc ttcaacggca tcggcgtgac
5161 ccagaatgtg ctgtacgaga accagaagct gatcgccaat cagttcaact ccgccatcgg
5221 caagatccag gactccctgt ccagcaccgc cagcgccctg ggcaagctgc aggatgtggt
5281 gaatcagaac gcccaggccc tgaataccct ggtgaagcag ctgtccagca acttcggcgc
5341 catctccagc gtgctgaatg atatcctgag ccgcctggac aaggtggagg ctgaggtgca
5401 gatcgatagg ctgatcaccg gccgcctgca gtccctgcag acctacgtga cccagcagct
5461 gatcagggct gctgagatca gggccagcgc caatctggct gctaccaaga tgtccgagtg
5521 cgtgctggga cagagcaaga gggtggactt ctgcggcaag ggctaccacc tgatgtcctt
5581 cccacagagc gccccacacg gagtggtgtt cctgcacgtg acctacgtgc cagcccagga
5641 gaagaacttc accaccgctc cagctatctg ccacgatggc aaggctcact tcccacgcga
5701 gggcgtgttc gtgtccaacg gcacccactg gttcgtgacc cagcgcaatt tctacgagcc
5761 ccagatcatc accaccgaca ataccttcgt gagcggcaac tgcgacgtgg tcatcggaat
5821 cgtgaacaat accgtgtacg atcccctgca gccagagctg gactccttca aggaggagct
5881 ggataagtac ttcaagaatc acaccagccc cgacgtggat ctgggcgaca tctccggcat
5941 caatgccagc gtggtgaaca tccagaagga gatcgaccgc ctgaacgagg tggccaagaa
6001 tctgaacgag tccctgatcg atctgcagga gctgggcaag tacgagcagt acatcaagtg
6061 gccatggtac atctggctgg gcttcatcgc cggcctgatc gccatcgtga tggtgaccat
6121 catgctgtgc tgcatgacct cctgctgcag ctgcctgaag ggctgctgct cctgcggcag
6181 ctgctgcaag ttcgatgagg acgatagcga gcccgtgctg aagggcgtca aactgcacta
6241 tacatagctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
6301 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
6361 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
6421 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
6481 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
6541 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
6601 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
6661 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
6721 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
6781 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
6841 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
6901 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
6961 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
7021 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
7081 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
7141 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
7201 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
7261 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
7321 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
7381 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
7441 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
7501 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
7561 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
7621 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
7681 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
7741 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
7801 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
7861 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
7921 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
7981 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
8041 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
8101 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
8161 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
8221 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
8281 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
8341 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
8401 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
8461 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
8521 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
8581 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
8641 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
8701 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
8761 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
8821 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
8881 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
8941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
9001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
9061 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
9121 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggt
//
