| 出品公司: | Thermofisher |
|---|---|
| 载体名称: | pTet-Splice |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | Tet |
| 载体大小: | 5.2 kb |
| 5' 测序引物及序列: | pBluescriptKS primer:TCGAGGTCGACGGTATC |
| 3' 测序引物及序列: | pBluescriptSK Primer:TCTAGAACTAGTGGATC |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 这个质粒含Tet操纵子，这个操纵子控制质粒上面的CMV启动子，所以当这个质粒与激活质粒pTet-tTAK共表达的时候，目的蛋白可以在Doxycycline多西环素（一种四环素）的诱导下进行表达。所以这个质粒是哺乳细胞四环素诱导表达质粒。 |
| 产品目录号: | #217568 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 可诱导 |
| 病毒/非病毒: | 非病毒 |
买家导航
pTetSplice载体基本信息
pTetSplice载体图谱质粒图谱和多克隆位点信息
pTetSplice载体简介
pTetSplice载体序列
LOCUS pTet-Splice 5178 bp ds-DNA circular SYN 14-FEB-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5178)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, February 14, 2019 from SnapGene Viewer 3.2.1
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5178
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 15..285
/bound_moiety="tetracycline repressor TetR"
/note="tetracycline response element"
/note="contains seven copies of the tetracycline
operator tetO"
promoter 318..355
/note="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 484..500
/note="KS primer"
/note="common sequencing primer, one of multiple
similar variants"
primer_bind complement(534..550)
/note="SK primer"
/note="common sequencing primer, one of multiple
similar variants"
intron 753..818
/note="small t intron"
/note="simian virus 40 (SV40) small t antigen intron"
CDS 948..968
/codon_start=1
/product="nuclear localization signal of SV40
large T antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
polyA_signal 1393..1527
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(2319..2337)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2347..2363)
/note="M13 fwd"
/note="common sequencing primer, one of multiple
similar variants"
rep_origin 2505..2960
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication;
arrow indicates direction of (+) strand synthesis"
promoter 2986..3090
/gene="bla"
/note="AmpR promoter"
CDS 3091..3951
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin,
and related antibiotics"
rep_origin 4122..4710
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4998..5019
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the
presence of cAMP."
promoter 5034..5064
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 5072..5088
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator
to inhibit transcription in E. coli.
This inhibition can be relieved by adding
lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5096..5112
/note="M13 rev"
/note="common sequencing primer, one of multiple
similar variants"
promoter 5133..5151
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
301 ctcggtaccc gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
361 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
421 ccgggaccga tccagcctcc gcggccccga attcgagctc ggtacccggg gatcctctag
481 agtcgaggtc gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac
541 tagttctaga gcactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
601 ggtgccctta aacgcctggt gctacgcctg aataagtgat aataagcgga tgaatggcag
661 aaattcgccg gaggcggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
721 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
781 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
841 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
901 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
961 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
1021 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
1081 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
1141 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
1201 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
1261 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
1321 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
1381 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
1441 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
1501 atcaatgtat cttatcatgt ctggatcccc aggaagctcc tctgtgtcct cataaaccct
1561 aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc tctgtgtcct
1621 cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca cagaccgctt
1681 tctaagggta attttaaaat atctgggaag tcccttccac tgctgtgttc cagaagtgtt
1741 ggtaaacagc ccacaaatgt caacagcaga aacatacaag ctgtcagctt tgcacaaggg
1801 cccaacaccc tgctcatcaa gaagcactgt ggttgctgtg ttagtaatgt gcaaaacagg
1861 aggcacattt tccccacctg tgtaggttcc aaaatatcta gtgttttcat ttttacttgg
1921 atcaggaacc cagcactcca ctggataagc attatcctta tccaaaacag ccttgtggtc
1981 agtgttcatc tgctgactgt caactgtagc attttttggg gttacagttt gagcaggata
2041 tttggtcctg tagtttgcta acacaccctg cagctccaaa ggttccccac caacagcaaa
2101 aaaatgaaaa tttgaccctt gaatgggttt tccagcacca ttttcatgag ttttttgtgt
2161 ccctgaatgc aagtttaaca tagcagttac cccaataacc tcagttttaa cagtaacagc
2221 ttcccacatc aaaatatttc cacaggttaa gtcctcattt aaattaggca aaggaattgc
2281 tctagagcgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacgc
2341 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
2401 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
2461 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
2521 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
2581 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
2701 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
2821 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
2881 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
2941 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
3001 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
3061 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
3121 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
3181 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
3241 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
3301 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
3361 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
3421 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
3481 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
3541 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
3601 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
3661 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
3721 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
3781 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
3841 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
3901 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
3961 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
4021 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
4081 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
4141 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
4201 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
4261 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
4321 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
4381 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
4441 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
4501 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
4561 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
4621 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
4681 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
4741 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
4801 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
4861 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
4921 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
4981 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
5041 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
5101 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
5161 ctgggtaccg ggcccccc
//
