pMDLg/pRRE

LOCUS       pMDLg/pRRE	8895 bp 	DNA	SYN
DEFINITION  pMDLg/pRRE
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="pMDLg/pRRE"
                     /mol_type="other DNA"
     misc_feature    complement(40..59)
                     /label="SV40pro_F_primer"
     promoter        162..738
                     /label="CMV_immearly_promoter"
     misc_feature    241..528
                     /label="CAG_enhancer"
     misc_feature    695..715
                     /label="CMV_fwd_primer"
     promoter        696..765
                     /label="CMV_promoter"
     promoter        708..827
                     /label="CMV2_promoter"
     misc_feature    739..758
                     /label="pCEP_fwd_primer"
     misc_feature    741..765
                     /label="LNCX_primer"
     misc_feature    1015..1398
                     /label="bGlob_int"
     gene            1437..2945
                     /label="Gag_HIV (variant)"
                     /gene="Gag_HIV (variant)"
     CDS             1437..2945
                     /label="ORF frame 3"
     CDS             3011..5749
                     /label="ORF frame 2"
     misc_feature    5438..5453
                     /label="cPPT"
     CDS             5694..6212
                     /label="ORF frame 3"
     misc_feature    5774..6007
                     /label="RRE"
     rep_origin      complement(7126..7745)
                     /label="pBR322_origin"
     gene            complement(7900..8760)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(7900..8760)
                     /label="ORF frame 1"
     promoter        complement(8802..8830)
                     /label="AmpR_promoter"
ORIGIN
    1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
   61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
  121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
  181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
  241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
  301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
  361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
  421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
  481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
  541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
  601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
  661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
  721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
  781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
  841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
  901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
  961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
 1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
 1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
 1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
 1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
 1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
 1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
 1381 ctggcccatc actttggcaa agcacgtgag atctgaattc gagatctgcc gccgccatgg
 1441 gtgcgagagc gtcagtatta agcgggggag aattagatcg atgggaaaaa attcggttaa
 1501 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
 1561 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
 1621 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
 1681 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
 1741 acaagataga ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcagctgaca
 1801 caggacacag caatcaggtc agccaaaatt accctatagt gcagaacatc caggggcaaa
 1861 tggtacatca ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga
 1921 aggctttcag cccagaagtg atacccatgt tttcagcatt atcagaagga gccaccccac
 1981 aagatttaaa caccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa
 2041 aagagaccat caatgaggaa gctgcagaat gggatagagt gcatccagtg catgcagggc
 2101 ctattgcacc aggccagatg agagaaccaa ggggaagtga catagcagga actactagta
 2161 ctagtaccct tcaggaacaa ataggatgga tgacacataa tccacctatc ccagtaggag
 2221 aaatctataa aagatggata atcctgggat taaataaaat agtaagaatg tatagcccta
 2281 ccagcattct ggacataaga caaggaccaa aggaaccctt tagagactat gtagaccgat
 2341 tctataaaac tctaagagcc gagcaagctt cacaagaggt aaaaaattgg atgacagaaa
 2401 ccttgttggt ccaaaatgcg aacccagatt gtaagactat tttaaaagca ttgggaccag
 2461 gagcgacact agaagaaatg atgacagcat gtcagggagt ggggggaccc ggccataaag
 2521 caagagtttt ggctgaagca atgagccaag taacaaatcc agctaccata atgatacaga
 2581 aaggcaattt taggaaccaa agaaagactg ttaagtgttt caattgtggc aaagaagggc
 2641 acatagccaa aaattgcagg gcccctagga aaaagggctg ttggaaatgt ggaaaggaag
 2701 gacaccaaat gaaagattgt actgagagac aggctaattt tttagggaag atctggcctt
 2761 cccacaaggg aaggccaggg aattttcttc agagcagacc agagccaaca gccccaccag
 2821 aagagagctt caggtttggg gaagagacaa caactccctc tcagaagcag gagccgatag
 2881 acaaggaact gtatccttta gcttccctca gatcactctt tggcagcgac ccctcgtcac
 2941 aataaagata ggggggcaat taaaggaagc tctattagat acaggagcag atgatacagt
 3001 attagaagaa atgaatttgc caggaagatg gaaaccaaaa atgatagggg gaattggagg
 3061 ttttatcaaa gtaggacagt atgatcagat actcatagaa atctgcggac ataaagctat
 3121 aggtacagta ttagtaggac ctacacctgt caacataatt ggaagaaatc tgttgactca
 3181 gattggctgc actttaaatt ttcccattag tcctattgag actgtaccag taaaattaaa
 3241 gccaggaatg gatggcccaa aagttaaaca atggccattg acagaagaaa aaataaaagc
 3301 attagtagaa atttgtacag aaatggaaaa ggaaggaaaa atttcaaaaa ttgggcctga
 3361 aaatccatac aatactccag tatttgccat aaagaaaaaa gacagtacta aatggagaaa
 3421 attagtagat ttcagagaac ttaataagag aactcaagat ttctgggaag ttcaattagg
 3481 aataccacat cctgcagggt taaaacagaa aaaatcagta acagtactgg atgtgggcga
 3541 tgcatatttt tcagttccct tagataaaga cttcaggaag tatactgcat ttaccatacc
 3601 tagtataaac aatgagacac cagggattag atatcagtac aatgtgcttc cacagggatg
 3661 gaaaggatca ccagcaatat tccagtgtag catgacaaaa atcttagagc cttttagaaa
 3721 acaaaatcca gacatagtca tctatcaata catggatgat ttgtatgtag gatctgactt
 3781 agaaataggg cagcatagaa caaaaataga ggaactgaga caacatctgt tgaggtgggg
 3841 atttaccaca ccagacaaaa aacatcagaa agaacctcca ttcctttgga tgggttatga
 3901 actccatcct gataaatgga cagtacagcc tatagtgctg ccagaaaagg acagctggac
 3961 tgtcaatgac atacagaaat tagtgggaaa attgaattgg gcaagtcaga tttatgcagg
 4021 gattaaagta aggcaattat gtaaacttct taggggaacc aaagcactaa cagaagtagt
 4081 accactaaca gaagaagcag agctagaact ggcagaaaac agggagattc taaaagaacc
 4141 ggtacatgga gtgtattatg acccatcaaa agacttaata gcagaaatac agaagcaggg
 4201 gcaaggccaa tggacatatc aaatttatca agagccattt aaaaatctga aaacaggaaa
 4261 atatgcaaga atgaagggtg cccacactaa tgatgtgaaa caattaacag aggcagtaca
 4321 aaaaatagcc acagaaagca tagtaatatg gggaaagact cctaaattta aattacccat
 4381 acaaaaggaa acatgggaag catggtggac agagtattgg caagccacct ggattcctga
 4441 gtgggagttt gtcaataccc ctcccttagt gaagttatgg taccagttag agaaagaacc
 4501 cataatagga gcagaaactt tctatgtaga tggggcagcc aatagggaaa ctaaattagg
 4561 aaaagcagga tatgtaactg acagaggaag acaaaaagtt gtccccctaa cggacacaac
 4621 aaatcagaag actgagttac aagcaattca tctagctttg caggattcgg gattagaagt
 4681 aaacatagtg acagactcac aatatgcatt gggaatcatt caagcacaac cagataagag
 4741 tgaatcagag ttagtcagtc aaataataga gcagttaata aaaaaggaaa aagtctacct
 4801 ggcatgggta ccagcacaca aaggaattgg aggaaatgaa caagtagatg ggttggtcag
 4861 tgctggaatc aggaaagtac tatttttaga tggaatagat aaggcccaag aagaacatga
 4921 gaaatatcac agtaattgga gagcaatggc tagtgatttt aacctaccac ctgtagtagc
 4981 aaaagaaata gtagccagct gtgataaatg tcagctaaaa ggggaagcca tgcatggaca
 5041 agtagactgt agcccaggaa tatggcagct agattgtaca catttagaag gaaaagttat
 5101 cttggtagca gttcatgtag ccagtggata tatagaagca gaagtaattc cagcagagac
 5161 agggcaagaa acagcatact tcctcttaaa attagcagga agatggccag taaaaacagt
 5221 acatacagac aatggcagca atttcaccag tactacagtt aaggccgcct gttggtgggc
 5281 ggggatcaag caggaatttg gcattcccta caatccccaa agtcaaggag taatagaatc
 5341 tatgaataaa gaattaaaga aaattatagg acaggtaaga gatcaggctg aacatcttaa
 5401 gacagcagta caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
 5461 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
 5521 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
 5581 agtttggaaa ggaccagcaa agctcctctg gaaaggtgaa ggggcagtag taatacaaga
 5641 taatagtgac ataaaagtag tgccaagaag aaaagcaaag atcatcaggg attatggaaa
 5701 acagatggca ggtgatgatt gtgtggcaag tagacaggat gaggattaac acatggaatt
 5761 ccggagcggc cgcaggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
 5821 gcgcagcctc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
 5881 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
 5941 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
 6001 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
 6061 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
 6121 gggacagaga aattaacaat tacacaagct tccgcggaat tcaccccacc agtgcaggct
 6181 gcctatcaga aagtggtggc tggtgtggct aatgccctgg cccacaagtt tcactaagct
 6241 cgcttccttg ctgtccaatt tctattaaag gttccttggt tccctaagtc caactactaa
 6301 actgggggat attatgaagg gccttgagca tctggattct gcctaataaa aaacatttat
 6361 tttcattgca atgatgtatt taaattattt ctgaatattt tactaaaaag ggaatgtggg
 6421 aggtcagtgc atttaaaaca taaagaaatg aagagctagt tcaaaccttg ggaaaataca
 6481 ctatatctta aactccatga aagaaggtga ggctgcaaac agctaatgca cattggcaac
 6541 agccctgatg cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt
 6601 ggaggttaaa gtttggctat gctgtatttt acattactta ttgttttagc tgtcctcatg
 6661 aatgtctttt cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt
 6721 ctcttgctta gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat
 6781 ggtttctcct cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact
 6841 tgaagaagga aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc
 6901 ccccactcac agtgacccgg aatccctcga catggcagtc tagcactagt gcggccgcag
 6961 atctgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
 7021 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
 7081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
 7141 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
 7201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
 7261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
 7321 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
 7381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
 7441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
 7501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
 7561 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
 7621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
 7681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
 7741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
 7801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
 7861 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
 7921 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
 7981 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
 8041 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
 8101 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
 8161 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
 8221 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
 8281 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
 8341 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
 8401 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
 8461 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
 8521 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
 8581 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
 8641 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
 8701 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
 8761 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
 8821 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
 8881 agtgccacct gacgt
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