| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pLentG-KOSM |
| 质粒类型: | 慢病毒系统载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 13kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | GFP |
| 载体抗性: | 氨苄青霉素（Ampicillian） |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pLentG-KOSM is a simple lentiviral vector in which defined factors are in-frame fused into a single open
reading frame (ORF) via self-cleaving 2A peptides and are controlled by a CMV promoter. The mouse
transcription factor ORF is followed by IRES-GFP as a reporter for viral transduction. This polycistronic
expression system can efficiently reprogram mouse or human somatic cells into iPS cells; for example, in
infected MEF (GFP+), the reprogramming efficiency is around 1%, which is at least 10-fold higher than
the reprogramming method using a pool of viruses in which each virus expresses a different stem cell
factor gene. Notably, most of the iPS cells generated using the pLentG-KOSM system contain only a
single copy of the viral vector (Ref. 1).
载体序列
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
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