出品公司: | 爱迪基因 |
---|---|
载体名称: | FUW-M2rtTA |
质粒类型: | 慢病毒载体;四环素调控载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | Ubc |
载体大小: | 7979 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 |
宿主细胞(系): | 大部分哺乳动物细胞 |
备注: | FUW-M2rtTA vector expressing the reverse tetracycline transactivator |
产品目录号: | #20342 |
稳定性: | 稳表达 |
组成型/诱导型: | 诱导型 |
病毒/非病毒: | 慢病毒 |
买家导航
FUW-M2rtTA载体质粒基本信息
FUW-M2rtTA质粒图谱载体图谱和FUW-M2rtTA载体序列质粒序列多克隆位点信息
FUW-M2rtTA质粒载体简介
Lentiviral plasmid expressing the reverse tetracycline transactivator.
FUW-M2rtTA质粒序列载体序列
LOCUS FUW-M2rtTA 7979 bp DNA SYN DEFINITION FUW-M2rtTA ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7979 /organism="FUW-M2rtTA" /mol_type="other DNA" misc_feature 7..594 /label="WPRE" CDS 95..1144 /label="ORF frame 2" CDS 108..620 /label="ORF frame 3" misc_feature complement(597..613) /label="pBluescriptKS_primer" misc_feature 781..796 /label="cPPT" misc_feature 781..802 /label="U3PPT" misc_feature 1118..1298 /label="HIV-1_5_LTR" misc_feature 1118..1298 /label="truncHIV-1_3_LTR" misc_feature complement(1324..1341) /label="BGH_rev_primer" gene complement(2346..3206) /label="Ampicillin" /gene="Ampicillin" CDS complement(2346..3206) /label="ORF frame 1" promoter complement(3248..3276) /label="AmpR_promoter" promoter 3578..4154 /label="CMV_immearly_promoter" misc_feature 3657..3944 /label="CAG_enhancer" misc_feature 4111..4131 /label="CMV_fwd_primer" misc_feature 4174..4354 /label="truncHIV-1_3_LTR" misc_feature 4174..4354 /label="HIV-1_5_LTR" misc_feature 4465..4509 /label="HIV-1_psi_pack" CDS 4897..5784 /label="ORF frame 1" misc_feature 5019..5252 /label="RRE" misc_feature 5783..5798 /label="cPPT" promoter 5956..7172 /label="hUbC_promoter" CDS 7201..7947 /label="ORF frame 1" ORIGIN 1 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 61 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 121 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 181 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 241 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 301 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 361 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct 421 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 481 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 541 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata 601 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac 661 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac 721 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 781 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga 841 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc 901 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga 961 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca 1021 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt 1081 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat 1141 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 1201 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 1261 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat 1321 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 1381 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 1441 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 1501 gggaggattg ggaagacaat agcaggcatg tgagcaaaag gccagcaaaa ggccaggaac 1561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 1621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 1681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 2041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 2101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 2161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 2221 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 2281 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 2341 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 2401 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 2461 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 2521 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 2581 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 2641 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 2701 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2761 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2821 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2881 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2941 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 3001 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 3061 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 3121 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 3181 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 3241 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 3301 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc 3361 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt 3421 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta 3481 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg 3541 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt 3601 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat 3661 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa 3721 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg 3781 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc 3841 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct 3901 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga 3961 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa 4021 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc 4081 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg 4141 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga 4201 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 4261 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 4321 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 4381 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 4441 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 4501 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 4561 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 4621 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 4681 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 4741 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 4801 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 4861 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa 4921 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag 4981 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc 5041 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga 5101 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa 5161 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct 5221 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc 5281 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt 5341 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata 5401 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa 5461 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata 5521 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt 5581 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca 5641 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga 5701 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat 5761 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga 5821 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac 5881 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa 5941 cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc 6001 cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc 6061 cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta 6121 tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc 6181 agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc 6241 gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc 6301 gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt 6361 gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg 6421 gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga 6481 actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac 6541 gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga 6601 acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg 6661 gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg 6721 tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga 6781 gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg 6841 gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc 6901 tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga 6961 ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt 7021 tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg 7081 aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta 7141 aattctggcc gtttttggct tttttgttag acgaagcttg ggcccgggaa ttaattcacc 7201 atgtctagac tggacaagag caaagtcata aacggcgctc tggaattact caatggagtc 7261 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc 7321 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg 7381 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg 7441 aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat 7501 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg 7561 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt 7621 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca 7681 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag 7741 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag 7801 ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc 7861 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat 7921 tttgaccttg acatgctccc cgggtaacta agtaaggatc aattcgatat caagcttat //
FUW-M2rtTA载体图谱质粒图谱pdf版和FUW-M2rtTA载体序列质粒序列等相关资料下载
FUW-M2rtTA质粒载体应用举例
- 上一篇:pCMV-dR8.91
- 下一篇:pLentG-KOSM