pCMV-dR8.91

LOCUS       pCMV-dR8.91	12150 bp 	DNA	SYN
DEFINITION  pCMV-dR8.91
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..12150
                     /organism="pCMV-dR8.91"
                     /mol_type="other DNA"
     promoter        22..574
                     /label="CMV_immearly_promoter"
     misc_feature    77..364
                     /label="CAG_enhancer"
     misc_feature    531..551
                     /label="CMV_fwd_primer"
     promoter        532..601
                     /label="CMV_promoter"
     promoter        544..663
                     /label="CMV2_promoter"
     misc_feature    575..594
                     /label="pCEP_fwd_primer"
     misc_feature    577..601
                     /label="LNCX_primer"
     gene            855..2357
                     /label="Gag_HIV (variant)"
                     /gene="Gag_HIV (variant)"
     CDS             855..2357
                     /label="ORF frame 3"

     CDS             2423..5161
                     /label="ORF frame 2"

     misc_feature    4850..4865
                     /label="cPPT"

     CDS             5613..6761
                     /label="ORF frame 3"

     misc_feature    5735..5968
                     /label="RRE"

     misc_feature    6491..6520
                     /label="HIV_Rev_NES"

     misc_feature    complement(7141..7158)
                     /label="Sp6_primer"

     gene            7396..7659
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"

     misc_feature    complement(7448..7467)
                     /label="pBRrevBam_primer"

     misc_feature    7800..7822
                     /label="pGEX_3_primer"

     promoter        7981..8009
                     /label="AmpR_promoter"

     gene            8051..8911
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             8051..8911
                     /label="ORF frame 2"

     rep_origin      9066..9685
                     /label="pBR322_origin"

     misc_feature    complement(9911..9931)
                     /label="pBABE_3_primer"

     misc_feature    complement(9917..10370)
                     /label="SV40_enhancer"

     promoter        9929..10197
                     /label="SV40_promoter"

     rep_origin      10096..10173
                     /label="SV40_origin"

     misc_feature    10158..10177
                     /label="SV40pro_F_primer"

     misc_feature    11378..11393
                     /label="SV40_int"

     misc_feature    11477..11492
                     /label="SV40_int"

     misc_feature    11498..11545
                     /label="SV40_3_splice"

     CDS             11622..12095
                     /label="ORF frame 3"

     misc_feature    11673..11693
                     /label="SV40 NLS"

     misc_feature    11679..11693
                     /label="NLS"

     promoter        12148..574
                     /label="CMV_immearly_promoter"

ORIGIN
    1 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
   61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
  121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
  181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
  241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
  301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
  361 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
  421 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
  481 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
  541 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
  601 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
  661 cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga gtgacgtaag
  721 taccgcctat agagtctata ggcccacccc cttggcttct tatgcgacgg atcgatcccg
  781 taataagctt cgaggtccgc ggccggccgc gttgacgcgc acggcaagag gcgaggggcg
  841 gcgactggtg agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgat
  901 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
  961 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
 1021 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
 1081 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
 1141 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag aaaaaagcac
 1201 agcaagcagc agctgacaca ggacacagca atcaggtcag ccaaaattac cctatagtgc
 1261 agaacatcca ggggcaaatg gtacatcagg ccatatcacc tagaacttta aatgcatggg
 1321 taaaagtagt agaagagaag gctttcagcc cagaagtgat acccatgttt tcagcattat
 1381 cagaaggagc caccccacaa gatttaaaca ccatgctaaa cacagtgggg ggacatcaag
 1441 cagccatgca aatgttaaaa gagaccatca atgaggaagc tgcagaatgg gatagagtgc
 1501 atccagtgca tgcagggcct attgcaccag gccagatgag agaaccaagg ggaagtgaca
 1561 tagcaggaac tactagtacc cttcaggaac aaataggatg gatgacacat aatccaccta
 1621 tcccagtagg agaaatctat aaaagatgga taatcctggg attaaataaa atagtaagaa
 1681 tgtatagccc taccagcatt ctggacataa gacaaggacc aaaggaaccc tttagagact
 1741 atgtagaccg attctataaa actctaagag ccgagcaagc ttcacaagag gtaaaaaatt
 1801 ggatgacaga aaccttgttg gtccaaaatg cgaacccaga ttgtaagact attttaaaag
 1861 cattgggacc aggagcgaca ctagaagaaa tgatgacagc atgtcaggga gtggggggac
 1921 ccggccataa agcaagagtt ttggctgaag caatgagcca agtaacaaat ccagctacca
 1981 taatgataca gaaaggcaat tttaggaacc aaagaaagac tgttaagtgt ttcaattgtg
 2041 gcaaagaagg gcacatagcc aaaaattgca gggcccctag gaaaaagggc tgttggaaat
 2101 gtggaaagga aggacaccaa atgaaagatt gtactgagag acaggctaat tttttaggga
 2161 agatctggcc ttcccacaag ggaaggccag ggaattttct tcagagcaga ccagagccaa
 2221 cagccccacc agaagagagc ttcaggtttg gggaagagac aacaactccc tctcagaagc
 2281 aggagccgat agacaaggaa ctgtatcctt tagcttccct cagatcactc tttggcagcg
 2341 acccctcgtc acaataaaga taggggggca attaaaggaa gctctattag atacaggagc
 2401 agatgataca gtattagaag aaatgaattt gccaggaaga tggaaaccaa aaatgatagg
 2461 gggaattgga ggttttatca aagtaagaca gtatgatcag atactcatag aaatctgcgg
 2521 acataaagct ataggtacag tattagtagg acctacacct gtcaacataa ttggaagaaa
 2581 tctgttgact cagattggct gcactttaaa ttttcccatt agtcctattg agactgtacc
 2641 agtaaaatta aagccaggaa tggatggccc aaaagttaaa caatggccat tgacagaaga
 2701 aaaaataaaa gcattagtag aaatttgtac agaaatggaa aaggaaggaa aaatttcaaa
 2761 aattgggcct gaaaatccat acaatactcc agtatttgcc ataaagaaaa aagacagtac
 2821 taaatggaga aaattagtag atttcagaga acttaataag agaactcaag atttctggga
 2881 agttcaatta ggaataccac atcctgcagg gttaaaacag aaaaaatcag taacagtact
 2941 ggatgtgggc gatgcatatt tttcagttcc cttagataaa gacttcagga agtatactgc
 3001 atttaccata cctagtataa acaatgagac accagggatt agatatcagt acaatgtgct
 3061 tccacaggga tggaaaggat caccagcaat attccagtgt agcatgacaa aaatcttaga
 3121 gccttttaga aaacaaaatc cagacatagt catctatcaa tacatggatg atttgtatgt
 3181 aggatctgac ttagaaatag ggcagcatag aacaaaaata gaggaactga gacaacatct
 3241 gttgaggtgg ggatttacca caccagacaa aaaacatcag aaagaacctc cattcctttg
 3301 gatgggttat gaactccatc ctgataaatg gacagtacag cctatagtgc tgccagaaaa
 3361 ggacagctgg actgtcaatg acatacagaa attagtggga aaattgaatt gggcaagtca
 3421 gatttatgca gggattaaag taaggcaatt atgtaaactt cttaggggaa ccaaagcact
 3481 aacagaagta gtaccactaa cagaagaagc agagctagaa ctggcagaaa acagggagat
 3541 tctaaaagaa ccggtacatg gagtgtatta tgacccatca aaagacttaa tagcagaaat
 3601 acagaagcag gggcaaggcc aatggacata tcaaatttat caagagccat ttaaaaatct
 3661 gaaaacagga aagtatgcaa gaatgaaggg tgcccacact aatgatgtga aacaattaac
 3721 agaggcagta caaaaaatag ccacagaaag catagtaata tggggaaaga ctcctaaatt
 3781 taaattaccc atacaaaagg aaacatggga agcatggtgg acagagtatt ggcaagccac
 3841 ctggattcct gagtgggagt ttgtcaatac ccctccctta gtgaagttat ggtaccagtt
 3901 agagaaagaa cccataatag gagcagaaac tttctatgta gatggggcag ccaataggga
 3961 aactaaatta ggaaaagcag gatatgtaac tgacagagga agacaaaaag ttgtccccct
 4021 aacggacaca acaaatcaga agactgagtt acaagcaatt catctagctt tgcaggattc
 4081 gggattagaa gtaaacatag tgacagactc acaatatgca ttgggaatca ttcaagcaca
 4141 accagataag agtgaatcag agttagtcag tcaaataata gagcagttaa taaaaaagga
 4201 aaaagtctac ctggcatggg taccagcaca caaaggaatt ggaggaaatg aacaagtaga
 4261 taaattggtc agtgctggaa tcaggaaagt actattttta gatggaatag ataaggccca
 4321 agaagaacat gagaaatatc acagtaattg gagagcaatg gctagtgatt ttaacctacc
 4381 acctgtagta gcaaaagaaa tagtagccag ctgtgataaa tgtcagctaa aaggggaagc
 4441 catgcatgga caagtagact gtagcccagg aatatggcag ctagattgta cacatttaga
 4501 aggaaaagtt atcttggtag cagttcatgt agccagtgga tatatagaag cagaagtaat
 4561 tccagcagag acagggcaag aaacagcata cttcctctta aaattagcag gaagatggcc
 4621 agtaaaaaca gtacatacag acaatggcag caatttcacc agtactacag ttaaggccgc
 4681 ctgttggtgg gcggggatca agcaggaatt tggcattccc tacaatcccc aaagtcaagg
 4741 agtaatagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
 4801 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
 4861 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
 4921 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
 4981 cagcagagat ccagtttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
 5041 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcatcag
 5101 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
 5161 acacatggaa ttctgcaaca actgctgttt atccatttca gaattgggtg tcgacatagc
 5221 agaataggcg ttactcgaca gaggagagca agaaatggag ccagtagatc ctagactaga
 5281 gccctggaag catccaggaa gtcagcctaa aactgcttgt accaattgct attgtaaaaa
 5341 gtgttgcttt cattgccaag tttgtttcat gacaaaagcc ttaggcatct cctatggcag
 5401 gaagaagcgg agacagcgac gaagagctca tcagaacagt cagactcatc aagcttctct
 5461 atcaaagcag taagtagtac atgtaatgca acctataata gtagcaatag tagcattagt
 5521 agtagcaata ataatagcaa tagttgtgtg gtccatagta atcatagaat ataggaaaat
 5581 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
 5641 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
 5701 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
 5761 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
 5821 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
 5881 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
 5941 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
 6001 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
 6061 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
 6121 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
 6181 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
 6241 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
 6301 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
 6361 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
 6421 gatccattcg attagtgaac ggatccttgg cacttatctg ggacgatctg cggagcctgt
 6481 gcctcttcag ctaccaccgc ttgagagact tactcttgat tgtaacgagg attgtggaac
 6541 ttctgggacg cagggggtgg gaagccctca aatattggtg gaatctccta caatattgga
 6601 gtcaggagct aaagaatagt gctgttagct tgctcaatgc cacagccata gcagtagctg
 6661 aggggacaga tagggttata gaagtagtac aaggagcttg tagagctatt cgccacatac
 6721 ctagaagaat aagacagggc ttggaaagga ttttgctata agctcgaggc cgccccggtg
 6781 accttcagac cttggcactg gaggtggccc ggcagaagcg cggcatcgtg gatcagtgct
 6841 gcaccagcat ctgctctctc taccaactgg agaactactg caactaggcc caccactacc
 6901 ctgtccaccc ctctgcaatg aataaaacct ttgaaagagc actacaagtt gtgtgtacat
 6961 gcgtgcatgt gcatatgtgg tgcgggggga acatgagtgg ggctggctgg agtggcgatg
 7021 ataagctgtc aaacatgaga attaattctt gaagacgaaa gggcctcgtg atacgcctat
 7081 ttttataggt taatgtcatg ataataatgg tttcttagtc tagaattaat tccgtgtatt
 7141 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
 7201 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
 7261 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
 7321 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
 7381 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
 7441 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
 7501 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
 7561 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
 7621 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
 7681 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
 7741 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
 7801 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
 7861 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
 7921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
 7981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
 8041 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
 8101 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
 8161 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
 8221 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
 8281 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
 8341 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
 8401 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
 8461 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
 8521 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
 8581 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
 8641 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
 8701 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
 8761 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
 8821 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
 8881 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
 8941 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
 9001 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
 9061 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
 9121 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
 9181 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
 9241 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
 9301 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
 9361 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
 9421 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
 9481 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
 9541 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
 9601 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
 9661 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
 9721 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
 9781 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
 9841 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
 9901 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
 9961 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
10021 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
10081 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
10141 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
10201 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
10261 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
10321 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
10381 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
10441 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
10501 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
10561 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
10621 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
10681 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
10741 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
10801 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
10861 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
10921 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
10981 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
11041 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
11101 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
11161 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
11221 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
11281 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
11341 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaaccc
11401 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
11461 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
11521 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
11581 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
11641 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
11701 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
11761 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
11821 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
11881 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
11941 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
12001 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
12061 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttggg
12121 ctgcaggaat taattcgagc tcgcccgaca
//