| 质粒名称: | pAAV2/8 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 112864 |
| 存储实验室: | James M. Wilson |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37度 |
|
插入基因信息 |
|
| 插入基因名称: | Rep2/Cap8 |
| 物种来源: | AAV |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Truncated P5 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | KS primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV2/8 was a gift from James M. Wilson (Addgene plasmid # 112864 ; http://n2t.net/addgene:112864 ; RRID:Addgene_112864) |
买家导航
pAAV28载体基本信息
pAAV28载体图谱质粒图谱和多克隆位点信息
pAAV28载体简介
pAAV28载体序列
LOCUS pAAV2/8 7336 bp ds-DNA circular SYN 21-AUG-2019
DEFINITION AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION .
VERSION .
KEYWORDS pAAV2/8
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7336)
TITLE AAV packaging plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7336)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 21, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7336
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(4404..4424)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4404..4422)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4443..4459)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4443..4459)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4456..4478)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4467..4483
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4491..4521)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4536..4557
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4674..4691)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4845..5433)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4925..4944)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5604..6464)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6227..6246
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6465..6569)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6595..7050)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6732..6753)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6944..6963
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7177..7199
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7191..7208
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 7192..7208
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7218..7237
/label=T7
/note="T7 promoter, forward primer"
promoter 7218..7236
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 7265..7281
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 7266..7282
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc
61 ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat
121 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag
181 cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggctct tttctttgtg
241 caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg
301 aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt
361 taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc
421 gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa
481 acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg
541 aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag
601 gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact
661 tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag
721 cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg
781 tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc
841 cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa
901 attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc
961 acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag
1021 accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc
1081 aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg
1141 aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc
1201 gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc
1261 aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg
1321 ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag
1381 gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg
1441 gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca
1501 gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg
1561 gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg
1621 aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc aaatatctgc
1681 ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt
1741 tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat gggaaaggtg
1801 ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg catctttgaa
1861 caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga
1921 caacctctct gagggcattc gcgagtggtg ggcgctgaaa cctggagccc cgaagcccaa
1981 agccaaccag caaaagcagg acgacggccg gggtctggtg cttcctggct acaagtacct
2041 cggacccttc aacggactcg acaaggggga gcccgtcaac gcggcggacg cagcggccct
2101 cgagcacgac aaggcctacg accagcagct gcaggcgggt gacaatccgt acctgcggta
2161 taaccacgcc gacgccgagt ttcaggagcg tctgcaagaa gatacgtctt ttgggggcaa
2221 cctcgggcga gcagtcttcc aggccaagaa gcgggttctc gaacctctcg gtctggttga
2281 ggaaggcgct aagacggctc ctggaaagaa gagaccggta gagccatcac cccagcgttc
2341 tccagactcc tctacgggca tcggcaagaa aggccaacag cccgccagaa aaagactcaa
2401 ttttggtcag actggcgact cagagtcagt tccagaccct caacctctcg gagaacctcc
2461 agcagcgccc tctggtgtgg gacctaatac aatggctgca ggcggtggcg caccaatggc
2521 agacaataac gaaggcgccg acggagtggg tagttcctcg ggaaattggc attgcgattc
2581 cacatggctg ggcgacagag tcatcaccac cagcacccga acctgggccc tgcccaccta
2641 caacaaccac ctctacaagc aaatctccaa cgggacatcg ggaggagcca ccaacgacaa
2701 cacctacttc ggctacagca ccccctgggg gtattttgac tttaacagat tccactgcca
2761 cttttcacca cgtgactggc agcgactcat caacaacaac tggggattcc ggcccaagag
2821 actcagcttc aagctcttca acatccaggt caaggaggtc acgcagaatg aaggcaccaa
2881 gaccatcgcc aataacctca ccagcaccat ccaggtgttt acggactcgg agtaccagct
2941 gccgtacgtt ctcggctctg cccaccaggg ctgcctgcct ccgttcccgg cggacgtgtt
3001 catgattccc cagtacggct acctaacact caacaacggt agtcaggccg tgggacgctc
3061 ctccttctac tgcctggaat actttccttc gcagatgctg agaaccggca acaacttcca
3121 gtttacttac accttcgagg acgtgccttt ccacagcagc tacgcccaca gccagagctt
3181 ggaccggctg atgaatcctc tgattgacca gtacctgtac tacttgtctc ggactcaaac
3241 aacaggaggc acggcaaata cgcagactct gggcttcagc caaggtgggc ctaatacaat
3301 ggccaatcag gcaaagaact ggctgccagg accctgttac cgccaacaac gcgtctcaac
3361 gacaaccggg caaaacaaca atagcaactt tgcctggact gctgggacca aataccatct
3421 gaatggaaga aattcattgg ctaatcctgg catcgctatg gcaacacaca aagacgacga
3481 ggagcgtttt tttcccagta acgggatcct gatttttggc aaacaaaatg ctgccagaga
3541 caatgcggat tacagcgatg tcatgctcac cagcgaggaa gaaatcaaaa ccactaaccc
3601 tgtggctaca gaggaatacg gtatcgtggc agataacttg cagcagcaaa acacggctcc
3661 tcaaattgga actgtcaaca gccagggggc cttacccggt atggtctggc agaaccggga
3721 cgtgtacctg cagggtccca tctgggccaa gattcctcac acggacggca acttccaccc
3781 gtctccgctg atgggcggct ttggcctgaa acatcctccg cctcagatcc tgatcaagaa
3841 cacgcctgta cctgcggatc ctccgaccac cttcaaccag tcaaagctga actctttcat
3901 cacgcaatac agcaccggac aggtcagcgt ggaaattgaa tgggagctgc agaaggaaaa
3961 cagcaagcgc tggaaccccg agatccagta cacctccaac tactacaaat ctacaagtgt
4021 ggactttgct gttaatacag aaggcgtgta ctctgaaccc cgccccattg gcacccgtta
4081 cctcacccgt aatctgtaat tgcctgttaa tcaataaacc ggttgattcg tttcagttga
4141 actttggtct ctgcgaaggg cgaattcgtt taaacctgca ggactagagg tcctgtatta
4201 gaggtcacgt gagtgttttg cgacattttg cgacaccatg tggtcacgct gggtatttaa
4261 gcccgagtga gcacgcaggg tctccatttt gaagcgggag gtttgaacgc gcagccgcca
4321 agccgaattc tgcagatatc catcacactg gcggccgctc gactagagcg gccgccaccg
4381 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
4441 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
4501 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
4561 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
4621 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
4681 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
4741 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
4801 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
4861 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
4921 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
4981 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
5041 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
5101 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
5161 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
5221 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
5281 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
5341 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
5401 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
5461 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
5521 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
5581 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
5641 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
5701 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
5761 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
5821 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
5881 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
5941 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
6001 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
6061 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
6121 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
6181 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
6241 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
6301 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
6361 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
6421 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
6481 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
6541 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
6601 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
6661 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
6721 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
6781 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
6841 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
6901 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
6961 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
7021 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
7081 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
7141 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
7201 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc
7261 tcgatcgagg tcgacggtat cgggggagct cgcagggtct ccattttgaa gcgggaggtt
7321 tgaacgcgca gccgcc
//
