质粒名称: | pDGM6 |
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出品公司: | 爱迪基因 |
目录编号: | 110660 |
存储实验室: | David Russell |
载体骨架基本信息 |
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载体名称: | pDG |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 21845 bp |
完整质粒大小: | 21842 bp |
载体修饰: | 包装/辅助质粒pDGM6,包括血清型6衣壳阅读框,用编码SwaI和ClaI末端限制性位点的寡核苷酸引物5′-atttaaatcagtatggctgccgatggt-3′和5′-atcgatgctatggcatgagataa-3′对AAV 6型血清克隆的衣壳基因进行PCR扩增,并将得到的DNA片段插入相同酶切的质粒pDG中。 |
载体类型: | AAV |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | AAV6 cap genes, AAV2 rep genes, adenovirus helper genes |
物种来源: | adenovirus, adeno-associated virus |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | SwaI(未破坏) |
3' 克隆位点: | ClaI(未破坏) |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pDGM6 was a gift from David Russell (Addgene plasmid # 110660 ; http://n2t.net/addgene:110660 ; RRID:Addgene_110660) |
参考文献部分: | Systemic delivery of genes to striated muscles using adeno-associated viral vectors. Gregorevic P, Blankinship MJ, Allen JM, Crawford RW, Meuse L, Miller DG, Russell DW, Chamberlain JS. Nat Med. 2004 Aug;10(8):828-34. doi: 10.1038/nm1085. Epub 2004 Jul 25. 10.1038/nm1085 PubMed 15273747 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDGM6 21829 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.. ACCESSION . VERSION . KEYWORDS pDGM6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 21829) AUTHORS Gregorevic P, Blankinship MJ, Allen JM, Crawford RW, Meuse L, Miller DG, Russell DW, Chamberlain JS TITLE Systemic delivery of genes to striated muscles using adeno-associated viral vectors. JOURNAL Nat Med. 2004 Aug;10(8):828-34. doi: 10.1038/nm1085. Epub 2004 Jul 25. PUBMED 15273747 REFERENCE 2 (bases 1 to 21829) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..21829 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 21..123 /label=ITR /note="inverted terminal repeat of human adenovirus serotype 5" misc_signal 211..361 /label=Ad5 Psi /note="packaging signal for adenovirus serotype 5" primer_bind 483..499 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 483..499 /label=pBluescriptSK /note="For pBluescript vector" regulatory 7501..7510 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" repeat_region 19726..19828 /label=ITR /note="inverted terminal repeat of human adenovirus serotype 5" rep_origin complement(20019..20607) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(20099..20118) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(20759..21619) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 21382..21401 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(21620..21724) /gene="bla" /label=AmpR promoter primer_bind 21792..21810 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 ggatccgaat tcttaattaa catcatcaat aatatacctt attttggatt gaagccaata 61 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg 121 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg 181 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg 241 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc 301 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa 361 tatttgtcta gggccgcggg gactttgacc gtttacgtgg agactcgccc aggtgttttt 421 ctcaggtgtt ttccgcgttc cgggtcaaag ttggcgtttt attattatag tcagggggat 481 cctctagaac tagtggatcc gtccctagaa gtaaaaaagg gaaaaaagag tgtgtttgtc 541 aaaataggag acaggtggtg gcaaccaggg acttataggg gaccttacat ctacagacca 601 acagatgccc ccttaccata tacaggaaga tatgacttaa attgggatag gtgggtcaca 661 atcaacggct ataaagtgtt atacagatcc ctcccctttc gtgaaagact cgccagagct 721 agacctcctt ggtgtatgct aactgagaaa gagaaagacg acatgaaaca acaggtacat 781 gattatattt atctaggaac aggaatgcac ttttggggaa aggttttcca taccaaggaa 841 ggggcagtgg ctggactgat agaacattat tctgcaaaaa cttatggtat gagttattat 901 gattagcctt tatttgccca accttgcggt tcccagggtt taaataagtt tatggttaca 961 aactgttctt aaaacaagga tgtgagacaa gtggtttcct gagttggttt ggtatcaaat 1021 gttctgatct gagctcttag tgttctattt tcctatgttc ttttggaatc tatccaagtc 1081 ttatgtaaat gcttatgtaa accataatat aaaagagtgc tgattttttg agtaaacttg 1141 caacagtcct aacattcttc tctcgtgtgt ttgtgtctgt tcgccatccc gtctccgctc 1201 gtcacttatc cttcactttt cagagggtcc ccccgcagat cccggtcacc ctcaggtcgg 1261 gacctgcaga agacgcccga gtgagcacgc agggtctcca ttttgaagcg ggaggtttga 1321 acgcgcagcc gccatgccgg ggttttacga gattgtgatt aaggtcccca gcgaccttga 1381 cgagcatctg cccggcattt ctgacagctt tgtgaactgg gtggccgaga aggaatggga 1441 gttgccgcca gattctgaca tggatctgaa tctgattgag caggcacccc tgaccgtggc 1501 cgagaagctg cagcgcgact ttctgacgga atggcgccgt gtgagtaagg ccccggaggc 1561 ccttttcttt gtgcaatttg agaagggaga gagctacttc cacatgcacg tgctcgtgga 1621 aaccaccggg gtgaaatcca tggttttggg acgtttcctg agtcagattc gcgaaaaact 1681 gattcagaga atttaccgcg ggatcgagcc gactttgcca aactggttcg cggtcacaaa 1741 gaccagaaat ggcgccggag gcgggaacaa ggtggtggat gagtgctaca tccccaatta 1801 cttgctcccc aaaacccagc ctgagctcca gtgggcgtgg actaatatgg aacagtattt 1861 aagcgcctgt ttgaatctca cggagcgtaa acggttggtg gcgcagcatc tgacgcacgt 1921 gtcgcagacg caggagcaga acaaagagaa tcagaatccc aattctgatg cgccggtgat 1981 cagatcaaaa acttcagcca ggtacatgga gctggtcggg tggctcgtgg acaaggggat 2041 tacctcggag aagcagtgga tccaggagga ccaggcctca tacatctcct tcaatgcggc 2101 ctccaactcg cggtcccaaa tcaaggctgc cttggacaat gcgggaaaga ttatgagcct 2161 gactaaaacc gcccccgact acctggtggg ccagcagccc gtggaggaca tttccagcaa 2221 tcggatttat aaaattttgg aactaaacgg gtacgatccc caatatgcgg cttccgtctt 2281 tctgggatgg gccacgaaaa agttcggcaa gaggaacacc atctggctgt ttgggcctgc 2341 aactaccggg aagaccaaca tcgcggaggc catagcccac actgtgccct tctacgggtg 2401 cgtaaactgg accaatgaga actttccctt caacgactgt gtcgacaaga tggtgatctg 2461 gtgggaggag gggaagatga ccgccaaggt cgtggagtcg gccaaagcca ttctcggagg 2521 aagcaaggtg cgcgtggacc agaaatgcaa gtcctcggcc cagatagacc cgactcccgt 2581 gatcgtcacc tccaacacca acatgtgcgc cgtgattgac gggaactcaa cgaccttcga 2641 acaccagcag ccgttgcaag accggatgtt caaatttgaa ctcacccgcc gtctggatca 2701 tgactttggg aaggtcacca agcaggaagt caaagacttt ttccggtggg caaaggatca 2761 cgtggttgag gtggagcatg aattctacgt caaaaagggt ggagccaaga aaagacccgc 2821 ccccagtgac gcagatataa gtgagcccaa acgggtgcgc gagtcagttg cgcagccatc 2881 gacgtcagac gcggaagctt cgatcaacta cgcagacagg taccaaaaca aatgttctcg 2941 tcacgtgggc atgaatctga tgctgtttcc ctgcagacaa tgcgagagaa tgaatcagaa 3001 ttcaaatatc tgcttcactc acggacagaa agactgttta gagtgctttc ccgtgtcaga 3061 atctcaaccc gtttctgtcg tcaaaaaggc gtatcagaaa ctgtgctaca ttcatcatat 3121 catgggaaag gtgccagacg cttgcactgc ctgcgatctg gtcaatgtgg atttggatga 3181 ctgcatcttt gaacaataaa tgatttaaat caggtatggc tgccgatggt tatcttccag 3241 attggctcga ggacaacctc tctgagggca ttcgcgagtg gtgggacttg aaacctggag 3301 ccccgaaacc caaagccaac cagcaaaagc aggacgacgg ccggggtctg gtgcttcctg 3361 gctacaagta cctcggaccc ttcaacggac tcgacaaggg ggagcccgtc aacgcggcgg 3421 atgcagcggc cctcgagcac gacaaggcct acgaccagca gctcaaagcg ggtgacaatc 3481 cgtacctgcg gtataaccac gccgacgccg agtttcagga gcgtctgcaa gaagatacgt 3541 cttttggggg caacctcggg cgagcagtct tccaggccaa gaagagggtt ctcgaacctt 3601 ttggtctggt tgaggaaggt gctaagacgg ctcctggaaa gaaacgtccg gtagagcagt 3661 cgccacaaga gccagactcc tcctcgggca ttggcaagac aggccagcag cccgctaaaa 3721 agagactcaa ttttggtcag actggcgact cagagtcagt ccccgaccca caacctctcg 3781 gagaacctcc agcaaccccc gctgctgtgg gacctactac aatggcttca ggcggtggcg 3841 caccaatggc agacaataac gaaggcgccg acggagtggg taatgcctca ggaaattggc 3901 attgcgattc cacatggctg ggcgacagag tcatcaccac cagcacccga acatgggcct 3961 tgcccaccta taacaaccac ctctacaagc aaatctccag tgcttcaacg ggggccagca 4021 acgacaacca ctacttcggc tacagcaccc cctgggggta ttttgatttc aacagattcc 4081 actgccattt ctcaccacgt gactggcagc gactcatcaa caacaattgg ggattccggc 4141 ccaagagact caacttcaag ctcttcaaca tccaagtcaa ggaggtcacg acgaatgatg 4201 gcgtcacgac catcgctaat aaccttacca gcacggttca agtcttctcg gactcggagt 4261 accagttgcc gtacgtcctc ggctctgcgc accagggctg cctccctccg ttcccggcgg 4321 acgtgttcat gattccgcag tacggctacc taacgctcaa caatggcagc caggcagtgg 4381 gacggtcatc cttttactgc ctggaatatt tcccatcgca gatgctgaga acgggcaata 4441 actttacctt cagctacacc ttcgaggacg tgcctttcca cagcagctac gcgcacagcc 4501 agagcctgga ccggctgatg aatcctctca tcgaccagta cctgtattac ctgaacagaa 4561 ctcagaatca gtccggaagt gcccaaaaca aggacttgct gtttagccgg gggtctccag 4621 ctggcatgtc tgttcagccc aaaaactggc tacctggacc ctgttaccgg cagcagcgcg 4681 tttctaaaac aaaaacagac aacaacaaca gcaactttac ctggactggt gcttcaaaat 4741 ataaccttaa tgggcgtgaa tctataatca accctggcac tgctatggcc tcacacaaag 4801 acgacaaaga caagttcttt cccatgagcg gtgtcatgat ttttggaaag gagagcgccg 4861 gagcttcaaa cactgcattg gacaatgtca tgatcacaga cgaagaggaa atcaaagcca 4921 ctaaccccgt ggccaccgaa agatttggga ctgtggcagt caatctccag agcagcagca 4981 cagaccctgc gaccggagat gtgcatgtta tgggagcctt acctggaatg gtgtggcaag 5041 acagagacgt atacctgcag ggtcctattt gggccaaaat tcctcacacg gatggacact 5101 ttcacccgtc tcctctcatg ggcggctttg gacttaagca cccgcctcct cagatcctca 5161 tcaaaaacac gcctgttcct gcgaatcctc cggcagagtt ttcggctaca aagtttgctt 5221 cattcatcac ccagtattcc acaggacaag tgagcgtgga gattgaatgg gagctgcaga 5281 aagaaaacag caaacgctgg aatcccgaag tgcagtatac atctaactat gcaaaatctg 5341 ccaacgttga tttcactgtg gacaacaatg gactttatac tgagcctcgc cccattggca 5401 cccgttacct cacccgtccc ctgtaattgt ctgttaatca ataaaccggt taattcgtgt 5461 cagttgaact ttggtctcat gtcgttatta tcttatctgg tcaccatagc aatcgatgca 5521 tgtccttggg tccggcctgc tgaatgcgca ggcggtcggc catgccccag gcttcgtttt 5581 gacatcggcg caggtctttg tagtagtctt gcatgagcct ttctaccggc acttcttctt 5641 ctccttcctc ttgtcctgca tctcttgcat ctatcgctgc ggcggcggcg gagtttggcc 5701 gtaggtggcg ccctcttcct cccatcgtgt gaccccgaag cccctcatcg gctgaagcag 5761 ggctaggtcg gcgacaacgc gctcggctaa tatggcctgc tgcacctgcg tgagggtaga 5821 ctggaagtca tccatgtcca caaagcggtg gtatgcgccc gtgttgatgg tgtaagtgca 5881 gttggccata acggaccagt taacggtctg gtgacccggc tgcgagagct cggtgtacct 5941 gagacgcgag taagccctcg agtcaaatac gtagtcgtta caagtccgca ccaggtactg 6001 gtatcccacc aaaaagtgcg gcggcggctg gcggtagagg ggccagcgta gggtggccgg 6061 ggctccgggg gcgagatctt ccaacataag gcgatgatat ccgtagatgt acctggacat 6121 ccaggtgatg ccggcggcgg tggtggaggc gcgcggaaag tcgcggacgc ggttccagat 6181 gttgcgcagc ggcaaaaagt gctccatggt cgggacgctc tggccggtca ggcgcgcgca 6241 atcgttgacg ctctagaccg tgcaaaagga gagcctgtaa gcgggcactc ttccgtggtc 6301 tggtggataa attcgcaagg gtatcatggc ggacgaccgg ggttcgagcc ccgtatccgg 6361 ccgtccgccg tgatccatgc ggttaccgcc cgcgtgtcga acccaggtgt gcgacgtcag 6421 acaacggggg agtgctcctt ttggcttcct tccaggcgcg gcggctgctg cgctagcttt 6481 tttggccact ggccgcgcgc agcgtaagcg gttaggctgg aaagcgaaag cattaagtgg 6541 ctcgctccct gtagccggag ggttattttc caagggttga gtcgcgggac ccccggttcg 6601 agtctcggac cggccggact gcggcgaacg ggggtttgtc tccccgtcat gcaagacccc 6661 gcttgcaaat tcctccggaa acagggacga gccccttttt tgcttttccc agatgcatcc 6721 ggtgctgcgg cagatgcgcc cccctcctca gcagcggcaa gagcaagagc agcggcagac 6781 atgcagggca ccctcccctc ctcctaccgc gtcaggaggg gcgacatccg cggttgacgc 6841 ggcagcagat ggtgattacg aacccccgcg gcgccgggcc cggcactacc tggacttgga 6901 ggagggcgag ggcctggcgc ggctaggagc gccctctcct gagcggcacc caagggtgca 6961 gctgaagcgt gatacgcgtg aggcgtacgt gccgcggcag aacctgtttc gcgaccgcga 7021 gggagaggag cccgaggaga tgcgggatcg aaagttccac gcagggcgcg agctgcggca 7081 tggcctgaat cgcgagcggt tgctgcgcga ggaggacttt gagcccgacg cgcgaaccgg 7141 gattagtccc gcgcgcgcac acgtggcggc cgccgacctg gtaaccgcat acgagcagac 7201 ggtgaaccag ggcgatcgca ccctttggcg catcccattc tccagtaact ttatgtccat 7261 gggcgcactc acagacctgg gccaaaacct tctctacgcc aactccgccc acgcgctaga 7321 catgactttt gaggtggatc ccatggacga gcccaccctt ctttatgttt tgtttgaagt 7381 ctttgacgtg gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg tgtacctgcg 7441 cacgcccttc tcggccggca acgccacaac ataaagaagc aagcaacatc aacaacagct 7501 gccgccatgg gctccagtga gcaggaactg aaagccattg tcaaagatct tggttgtggg 7561 ccatattttt tgggcaccta tgacaagcgc tttccaggct ttgtttctcc acacaagctc 7621 gcctgcgcca tagtcaatac ggccggtcgc gagactgggg gcgtacactg gatggccttt 7681 gcctggaacc cgcactcaaa aacatgctac ctctttgagc cctttggctt ttctgaccag 7741 cgactcaagc aggtttacca gtttgagtac gagtcactcc tgcgccgtag cgccattgct 7801 tcttcccccg accgctgtat aacgctggaa aagtccaccc aaagcgtaca ggggcccaac 7861 tcggccgcct gtggactatt ctgctgcatg tttctccacg cctttgccaa ctggccccaa 7921 actcccatgg atcacaaccc caccatgaac cttattaccg gggtacccaa ctccatgctc 7981 aacagtcccc aggtacagcc caccctgcgt cgcaaccagg aacagctcta cagcttcctg 8041 gagcgccact cgccctactt ccgcagccac agtgcgcaga ttaggagcgc cacttctttt 8101 tgtcacttga aaaacatgta aaaataatgt actagagaca ctttcaataa aggcaaatgc 8161 ttttatttgt acactctcgg gtgattattt acccccaccc ttgccgtctg cgccgtttaa 8221 aaatcaaagg ggttctgccg cgcatcgcta tgcgccactg gcagggacac gttgcgatac 8281 tggtgtttag tgctccactt aaactcaggc acaaccatcc gcggcagctc ggtgaagttt 8341 tcactccaca ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc cgatatcttg 8401 aagtcgcagt tggggcctcc gccctgcgcg cgcgagttgc gatacacagg gttgcagcac 8461 tggaacacta tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc ggagatcaga 8521 tccgcgtcca ggtcctccgc gttgctcagg gcgaacggag tcaactttgg tagctgcctt 8581 cccaaaaagg gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg catcaaaagg 8641 tgaccgtgcc cggtctgggc gttaggatac agcgcctgca taaaagcctt gatctgctta 8701 aaagccacct gagcctttgc gccttcagag aagaacatgc cgcaagactt gccggaaaac 8761 tgattggccg gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt ggagatctgc 8821 accacatttc ggccccaccg gttcttcacg atcttggcct tgctagactg ctccttcagc 8881 gcgcgctgcc cgttttcgct cgtcacatcc atttcaatca cgtgctcctt atttatcata 8941 atgcttccgt gtagacactt aagctcgcct tcgatctcag cgcagcggtg cagccacaac 9001 gcgcagcccg tgggctcgtg atgcttgtag gtcacctctg caaacgactg caggtacgcc 9061 tgcaggaatc gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt cagctgcaac 9121 ccgcggtgct cctcgttcag ccaggtcttg catacggccg ccagagcttc cacttggtca 9181 ggcagtagtt tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc catcagcgcg 9241 cgcgcagcct ccatgccctt ctcccacgca gacacgatcg gcacactcag cgggttcatc 9301 accgtaattt cactttccgc ttcgctgggc tcttcctctt cctcttgcgt ccgcatacca 9361 cgcgccactg ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc tttgccatgc 9421 ttgattagca ccggtgggtt gctgaaaccc accatttgta gcgccacatc ttctctttct 9481 tcctcgctgt ccacgattac ctctggtgat ggcgggcgct cgggcttggg agaagggcgc 9541 ttctttttct tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg ccgcgggctg 9601 ggtgtgcgcg gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga ctcgatacgc 9661 cgcctcatcc gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga cggggacgac 9721 acgtcctcca tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg ggtggtttcg 9781 cgctgctcct cttcccgact ggccatttcc ttctcctata ggcagaaaaa gatcatggag 9841 tcagtcgaga agaaggacag cctaaccgcc ccctctgagt tcgccaccac cgcctccacc 9901 gatgccgcca acgcgcctac caccttcccc gtcgaggcac ccccgcttga ggaggaggaa 9961 gtgattatcg agcaggaccc aggttttgta agcgaagacg acgaggaccg ctcagtacca 10021 acagaggata aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca agtcgggcgg 10081 ggggacgaaa ggcatggcga ctacctagat gtgggagacg acgtgctgtt gaagcatctg 10141 cagcgccagt gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt gcccctcgcc 10201 atagcggatg tcagccttgc ctacgaacgc cacctattct caccgcgcgt accccccaaa 10261 cgccaagaaa acggcacatg cgagcccaac ccgcgcctca acttctaccc cgtatttgcc 10321 gtgccagagg tgcttgccac ctatcacatc tttttccaaa actgcaagat acccctatcc 10381 tgccgtgcca accgcagccg agcggacaag cagctggcct tgcggcaggg cgctgtcata 10441 cctgatatcg cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg acgcgacgag 10501 aagcgcgcgg caaacgctct gcaacaggaa aacagcgaaa atgaaagtca ctctggagtg 10561 ttggtggaac tcgagggtga caacgcgcgc ctagccgtac taaaacgcag catcgaggtc 10621 acccactttg cctacccggc acttaaccta ccccccaagg tcatgagcac agtcatgagt 10681 gagctgatcg tgcgccgtgc gcagcccctg gagagggatg caaatttgca agaacaaaca 10741 gaggagggcc tacccgcagt tggcgacgag cagctagcgc gctggcttca aacgcgcgag 10801 cctgccgact tggaggagcg acgcaaacta atgatggccg cagtgctcgt taccgtggag 10861 cttgagtgca tgcagcggtt ctttgctgac ccggagatgc agcgcaagct agaggaaaca 10921 ttgcactaca cctttcgaca gggctacgta cgccaggcct gcaagatctc caacgtggag 10981 ctctgcaacc tggtctccta ccttggaatt ttgcacgaaa accgccttgg gcaaaacgtg 11041 cttcattcca cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg cgtttactta 11101 tttctatgct acacctggca gacggccatg ggcgtttggc agcagtgctt ggaggagtgc 11161 aacctcaagg agctgcagaa actgctaaag caaaacttga aggacctatg gacggccttc 11221 aacgagcgct ccgtggccgc gcacctggcg gacatcattt tccccgaacg cctgcttaaa 11281 accctgcaac agggtctgcc agacttcacc agtcaaagca tgttgcagaa ctttaggaac 11341 tttatcctag agcgctcagg aatcttgccc gccacctgct gtgcacttcc tagcgacttt 11401 gtgcccatta agtaccgcga atgccctccg ccgctttggg gccactgcta ccttctgcag 11461 ctagccaact accttgccta ccactctgac ataatggaag acgtgagcgg tgacggtcta 11521 ctggagtgtc actgtcgctg caacctatgc accccgcacc gctccctggt ttgcaattcg 11581 cagctgctta acgaaagtca aattatcggt acctttgagc tgcagggtcc ctcgcctgac 11641 gaaaagtccg cggctccggg gttgaaactc actccggggc tgtggacgtc ggcttacctt 11701 cgcaaatttg tacctgagga ctaccacgcc cacgagatta ggttctacga agaccaatcc 11761 cgcccgccta atgcggagct taccgcctgc gtcattaccc agggccacat tcttggccaa 11821 ttgcaagcca tcaacaaagc ccgccaagag tttctgctac gaaagggacg gggggtttac 11881 ttggaccccc agtccggcga ggagctcaac ccaatccccc cgccgccgca gccctatcag 11941 cagcagccgc gggcccttgc ttcccaggat ggcacccaaa aagaagctgc agctgccgcc 12001 gccacccacg gacgaggagg aatactggga cagtcaggca gaggaggttt tggacgagga 12061 ggaggaggac atgatggaag actgggagag cctagacgag gaagcttccg aggtcgaaga 12121 ggtgtcagac gaaacaccgt caccctcggt cgcattcccc tcgccggcgc cccagaaatc 12181 ggcaaccggt tccagcatgg ctacaacctc cgctcctcag gcgccgccgg cactgcccgt 12241 tcgccgaccc aaccgtagat gggacaccac tggaaccagg gccggtaagt ccaagcagcc 12301 gccgccgtta gcccaagagc aacaacagcg ccaaggctac cgctcatggc gcgggcacaa 12361 gaacgccata gttgcttgct tgcaagactg tgggggcaac atctccttcg cccgccgctt 12421 tcttctctac catcacggcg tggccttccc ccgtaacatc ctgcattact accgtcatct 12481 ctacagccca tactgcaccg gcggcagcgg cagcaacagc agcggccaca cagaagcaaa 12541 ggcgaccgga tagcaagact ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag 12601 gaggaggagc gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag cttagaaaca 12661 ggatttttcc cactctgtat gctatatttc aacagagcag gggccaagaa caagagctga 12721 aaataaaaaa caggtctctg cgatccctca cccgcagctg cctgtatcac aaaagcgaag 12781 atcagcttcg gcgcacgctg gaagacgcgg aggctctctt cagtaaatac tgcgcgctga 12841 ctcttaagga ctagtttcgc gccctttctc aaatttaagc gcgaaaacta cgtcatctcc 12901 agcggccaca cccggcgcca gcacctgttg tcagcgccat tatgagcaag gaaattccca 12961 cgccctacat gtggagttac cagccacaaa tgggacttgc ggctggagct gcccaagact 13021 actcaacccg aataaactac atgagcgcgg gaccccacat gatatcccgg gtcaacggaa 13081 tacgcgccca ccgaaaccga attctcctgg aacaggcggc tattaccacc acacctcgta 13141 ataaccttaa tccccgtagt tggcccgctg ccctggtgta ccaggaaagt cctgctccca 13201 ccactgtggt acttcccaga gacgcccagg ccgaagttca gatgactaac tcaggggcgc 13261 agcttgcggg cggctttcgt cacagggtgc ggtcgcccgg gcagggtata actcacctga 13321 caatcagagg gcgaggtatt cagctcaacg acgagtcggt gagctcctcg cttggtctcc 13381 gtccggacgg gacatttcag atcggcggcg ccggccgctc ttcattcacg cctcgtcagg 13441 caatcctaac tctgcagacc tcgtcctctg agccgcgctc tggaggcatt ggaactctgc 13501 aatttattga ggagtttgtg ccatcggtct actttaaccc cttctcggga cctcccggcc 13561 actatccgga tcaatttatt cctaactttg acgcggtaaa ggactcggcg gacggctacg 13621 actgaatgtt aagtggagag gcagagcaac tgcgcctgaa acacctggtc cactgtcgcc 13681 gccacaagtg ctttgcccgc gactccggtg agttttgcta ctttgaattg cccgaggatc 13741 atatcgaggg cccggcgcac ggcgtccggc ttaccgccca gggagagctt gcccgtagcc 13801 tgattcggga gtttacccag cgccccctgc tagttgagcg ggacagggga ccctgtgttc 13861 tcactgtgat ttgcaactgt cctaaccctg gattacatca agatctttgt tgccatctct 13921 gtgctgagta taataaatac agaaattaaa atatactggg gctcctatcg ccatcctgta 13981 aacgccaccg tcttcacccg cccaagcaaa ccaaggcgaa ccttacctgg tacttttaac 14041 atctctccct ctgtgattta caacagtttc aacccagacg gagtgagtct acgagagaac 14101 ctctccgagc tcagctactc catcagaaaa aacaccaccc tccttacctg ccgggaacgt 14161 acgagtgcgt caccggccgc tgcaccacac ctaccgcctg accgtaaacc agactttttc 14221 cggacagacc tcaataactc tgtttaccag aacaggaggt gagcttagaa aacccttagg 14281 gtattaggcc aaaggcgcag ctactgtggg gtttatgaac aattcaagca actctacggg 14341 ctattctaat tcaggtttct ctagaaatgg acggaattat tacagagcag cgcctgctag 14401 aaagacgcag ggcagcggcc gagcaacagc gcatgaatca agagctccaa gacatggtta 14461 acttgcacca gtgcaaaagg ggtatctttt gtctggtaaa gcaggccaaa gtcacctacg 14521 acagtaatac caccggacac cgccttagct acaagttgcc aaccaagcgt cagaaattgg 14581 tggtcatggt gggagaaaag cccattacca taactcagca ctcggtagaa accgaaggct 14641 gcattcactc accttgtcaa ggacctgagg atctctgcac ccttattaag accctgtgcg 14701 gtctcaaaga tcttattccc tttaactaat aaaaaaaaat aataaagcat cacttactta 14761 aaatcagtta gcaaatttct gtccagttta ttcagcagca cctccttgcc ctcctcccag 14821 ctctggtatt gcagcttcct cctggctgca aactttctcc acaatctaaa tggaatgtca 14881 gtttcctcct gttcctgtcc atccgcaccc actatcttca tgttgttgca gatgaagcgc 14941 gcaagaccgt ctgaagatac cttcaacccc gtgtatccat atgacacgga aaccggtcct 15001 ccaactgtgc cttttcttac tcctcccttt gtatccccca atgggtttca agagagtccc 15061 cctggggtac tctctttgcg cctatccgaa cctctagtta cctccaatgg catgcttgcg 15121 ctcaaaatgg gcaacggcct ctctctggac gaggccggca accttacctc ccaaaatgta 15181 accactgtga gcccacctct caaaaaaacc aagtcaaaca taaacctgga aatatctgca 15241 cccctcacag ttacctcaga agccctaact gtggctgccg ccgcacctct aatggtcgcg 15301 ggcaacacac tcaccatgca atcacaggcc ccgctaaccg tgcacgactc caaacttagc 15361 attgccaccc aaggacccct cacagtgtca gaaggaaagc tagccctgca aacatcaggc 15421 cccctcacca ccaccgatag cagtaccctt actatcactg cctcaccccc tctaactact 15481 gccactggta gcttgggcat tgacttgaaa gagcccattt atacacaaaa tggaaaacta 15541 ggactaaagt acggggctcc tttgcatgta acagacgacc taaacacttt gaccgtagca 15601 actggtccag gtgtgactat taataatact tccttgcaaa ctaaagttac tggagccttg 15661 ggttttgatt cacaaggcaa tatgcaactt aatgtagcag gaggactaag gattgattct 15721 caaaacagac gccttatact tgatgttagt tatccgtttg atgctcaaaa ccaactaaat 15781 ctaagactag gacagggccc tctttttata aactcagccc acaacttgga tattaactac 15841 aacaaaggcc tttacttgtt tacagcttca aacaattcca aaaagcttga ggttaaccta 15901 agcactgcca aggggttgat gtttgacgct acagccatag ccattaatgc aggagatggg 15961 cttgaatttg gttcacctaa tgcaccaaac acaaatcccc tcaaaacaaa aattggccat 16021 ggcctagaat ttgattcaaa caaggctatg gttcctaaac taggaactgg ccttagtttt 16081 gacagcacag gtgccattac agtaggaaac aaaaataatg ataagctaac tttgtggacc 16141 acaccagctc catctcctaa ctgtagacta aatgcagaga aagatgctaa actcactttg 16201 gtcttaacaa aatgtggcag tcaaatactt gctacagttt cagttttggc tgttaaaggc 16261 agtttggctc caatatctgg aacagttcaa agtgctcatc ttattataag atttgacgaa 16321 aatggagtgc tactaaacaa ttccttcctg gacccagaat attggaactt tagaaatgga 16381 gatcttactg aaggcacagc ctatacaaac gctgttggat ttatgcctaa cctatcagct 16441 tatccaaaat ctcacggtaa aactgccaaa agtaacattg tcagtcaagt ttacttaaac 16501 ggagacaaaa ctaaacctgt aacactaacc attacactaa acggtacaca ggaaacagga 16561 gacacaactc caagtgcata ctctatgtca ttttcatggg actggtctgg ccacaactac 16621 attaatgaaa tatttgccac atcctcttac actttttcat acattgccca agaataaaga 16681 atcgtttgtg ttatgtttca acgtgtttat ttttcaattg cagaaaattt caagtcattt 16741 ttcattcagt agtatagccc caccaccaca tagcttatac agatcaccgt accttaatca 16801 aactcacaga accctagtat tcaacctgcc acctccctcc caacacacag agtacacagt 16861 cctttctccc cggctggcct taaaaagcat catatcatgg gtaacagaca tattcttagg 16921 tgttatattc cacacggttt cctgtcgagc caaacgctca tcagtgatat taataaactc 16981 cccgggcagc tcacttaagt tcatgtcgct gtccagctgc tgagccacag gctgctgtcc 17041 aacttgcggt tgcttaacgg gcggcgaagg agaagtccac gcctacatgg gggtagagtc 17101 ataatcgtgc atcaggatag ggcggtggtg ctgcagcagc gcgcgaataa actgctgccg 17161 ccgccgctcc gtcctgcagg aatacaacat ggcagtggtc tcctcagcga tgattcgcac 17221 cgcccgcagc ataaggcgcc ttgtcctccg ggcacagcag cgcaccctga tctcacttaa 17281 atcagcacag taactgcagc acagcaccac aatattgttc aaaatcccac agtgcaaggc 17341 gctgtatcca aagctcatgg cggggaccac agaacccacg tggccatcat accacaagcg 17401 caggtagatt aagtggcgac ccctcataaa cacgctggac ataaacatta cctcttttgg 17461 catgttgtaa ttcaccacct cccggtacca tataaacctc tgattaaaca tggcgccatc 17521 caccaccatc ctaaaccagc tggccaaaac ctgcccgccg gctatacact gcagggaacc 17581 gggactggaa caatgacagt ggagagccca ggactcgtaa ccatggatca tcatgctcgt 17641 catgatatca atgttggcac aacacaggca cacgtgcata cacttcctca ggattacaag 17701 ctcctcccgc gttagaacca tatcccaggg aacaacccat tcctgaatca gcgtaaatcc 17761 cacactgcag ggaagacctc gcacgtaact cacgttgtgc attgtcaaag tgttacattc 17821 gggcagcagc ggatgatcct ccagtatggt agcgcgggtt tctgtctcaa aaggaggtag 17881 acgatcccta ctgtacggag tgcgccgaga caaccgagat cgtgttggtc gtagtgtcat 17941 gccaaatgga acgccggacg tagtcatatt tcctgaagca aaaccaggtg cgggcgtgac 18001 aaacagatct gcgtctccgg tctcgccgct tagatcgctc tgtgtagtag ttgtagtata 18061 tccactctct caaagcatcc aggcgccccc tggcttcggg ttctatgtaa actccttcat 18121 gcgccgctgc cctgataaca tccaccaccg cagaataagc cacacccagc caacctacac 18181 attcgttctg cgagtcacac acgggaggag cgggaagagc tggaagaacc atgttttttt 18241 ttttattcca aaagattatc caaaacctca aaatgaagat ctattaagtg aacgcgctcc 18301 cctccggtgg cgtggtcaaa ctctacagcc aaagaacaga taatggcatt tgtaagatgt 18361 tgcacaatgg cttccaaaag gcaaacggcc ctcacgtcca agtggacgta aaggctaaac 18421 ccttcagggt gaatctcctc tataaacatt ccagcacctt caaccatgcc caaataattc 18481 tcatctcgcc accttctcaa tatatctcta agcaaatccc gaatattaag tccggccatt 18541 gtaaaaatct gctccagagc gccctccacc ttcagcctca agcagcgaat catgattgca 18601 aaaattcagg ttcctcacag acctgtataa gattcaaaag cggaacatta acaaaaatac 18661 cgcgatcccg taggtccctt cgcagggcca gctgaacata atcgtgcagg tctgcacgga 18721 ccagcgcggc cacttccccg ccaggaacca tgacaaaaga acccacactg attatgacac 18781 gcatactcgg agctatgcta accagcgtag ccccgatgta agcttgttgc atgggcggcg 18841 atataaaatg caaggtgctg ctcaaaaaat caggcaaagc ctcgcgcaaa aaagaaagca 18901 catcgtagtc atgctcatgc agataaaggc aggtaagctc cggaaccacc acagaaaaag 18961 acaccatttt tctctcaaac atgtctgcgg gtttctgcat aaacacaaaa taaaataaca 19021 aaaaaacatt taaacattag aagcctgtct tacaacagga aaaacaaccc ttataagcat 19081 aagacggact acggccatgc cggcgtgacc gtaaaaaaac tggtcaccgt gattaaaaag 19141 caccaccgac agctcctcgg tcatgtccgg agtcataatg taagactcgg taaacacatc 19201 aggttgattc acatcggtca gtgctaaaaa gcgaccgaaa tagcccgggg gaatacatac 19261 ccgcaggcgt agagacaaca ttacagcccc cataggaggt ataacaaaat taataggaga 19321 gaaaaacaca taaacacctg aaaaaccctc ctgcctaggc aaaatagcac cctcccgctc 19381 cagaacaaca tacagcgctt ccacagcggc agccataaca gtcagcctta ccagtaaaaa 19441 agaaaaccta ttaaaaaaac accactcgac acggcaccag ctcaatcagt cacagtgtaa 19501 aaaagggcca agtgcagagc gagtatatat aggactaaaa aatgacgtaa cggttaaagt 19561 ccacaaaaaa cacccagaaa accgcacgcg aacctacgcc cagaaacgaa agccaaaaaa 19621 cccacaactt cctcaaatcg tcacttccgt tttcccacgt tacgtcactt cccattttaa 19681 gaaaactaca attcccaaca catacaagtt actccgccct aaaacctacg tcacccgccc 19741 cgttcccacg ccccgcgcca cgtcacaaac tccaccccct cattatcata ttggcttcaa 19801 tccaaaataa ggtatattat tgatgatgtt aattaagaat tcggatctgc gacgcgaggc 19861 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg 19921 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggccagcaa 19981 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 20041 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 20101 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 20161 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 20221 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 20281 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 20341 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 20401 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 20461 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 20521 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 20581 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 20641 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 20701 ttcacctaga tccttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 20761 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 20821 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 20881 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 20941 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 21001 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 21061 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 21121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 21181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 21241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 21301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 21361 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca 21421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 21481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 21541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 21601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 21661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 21721 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 21781 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatt //