pCX-DR

LOCUS       pCX-DR               11229 bp ds-DNA     circular SYN 30-OCT-2017
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11229)
  TITLE     Direct Submission
            http://www.biofeng.com/
FEATURES             Location/Qualifiers
     source          1..11229
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    196..655
                     /label=CaMV35S promoter
     regulatory      673..682
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             679..1353
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     misc_feature    1354..1410
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(1466..1771)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     terminator      2279..2531
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2553..2577
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3876..4505
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4939..6006
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6072..6266
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6610..6750
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6936..7524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7611..8405)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8830..8854
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8932..9106
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9146..10171)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10238..10915)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gaattcgagc tcggtaccca ctggattttg gttttaggaa ttagaaattt tattgataga
       61 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
      121 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac
      181 tcgaaattcg agctccaccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
      241 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
      301 ctccgctcat gatccgggga attaattccg atcctatctg tcacttcatc aaaaggacag
      361 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcattc
      421 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
      481 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
      541 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
      601 gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca agatcctcta
      661 gcgctaccgg tcgccaccat ggcctcctcc gagaacgtca tcaccgagtt catgcgcttc
      721 aaggtgcgca tggagggcac cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      781 ggccgcccct acgagggcca caacaccgtg aagctgaagg tgaccaaggg cggccccctg
      841 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag
      901 caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag
      961 cgcgtgatga acttcgagga cggcggcgtg gcgaccgtga cccaggactc ctccctgcag
     1021 gacggctgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc cgacggcccc
     1081 gtgatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     1141 ggcgtgctga agggcgagac ccacaaggcc ctgaagctga aggacggcgg ccactacctg
     1201 gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg ctactactac
     1261 gtggacgcca agctggacat cacctcccac aacgaggact acaccatcgt ggagcagtac
     1321 gagcgcaccg agggccgcca ccacctgttc ctgagatctc gagctcaagc ttcgaattct
     1381 gcagtcgacg gtaccgcggg cccgggatcc ccaatacttg tatgggtcga cctgcagact
     1441 ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt
     1501 tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca
     1561 cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt
     1621 aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc
     1681 gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct
     1741 ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa
     1801 gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc
     1861 cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag
     1921 atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa
     1981 tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat cagtatatat
     2041 tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc
     2101 ccatacaagt attggggatc caccggatct agataactga tcataatcag ccataccaca
     2161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2221 aaaatgaatg caattgttgt tgttgtgtga attacaggtg accagctcga atttccccga
     2281 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     2341 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     2401 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     2461 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     2521 gttactagat cgggaattaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     2581 agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg
     2641 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
     2701 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     2761 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
     2821 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
     2881 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
     2941 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
     3001 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
     3061 accacctagc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     3121 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     3181 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     3241 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     3301 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     3361 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     3421 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     3481 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     3541 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     3601 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     3661 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     3721 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     3781 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     3841 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     3901 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     3961 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     4021 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     4081 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     4141 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     4201 cttacgacat atgggcaacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     4261 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     4321 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     4381 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     4441 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     4501 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     4561 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     4621 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     4681 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     4741 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     4801 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     4861 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     4921 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     4981 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     5041 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     5101 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     5161 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     5221 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     5281 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     5341 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     5401 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     5461 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     5521 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     5581 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     5641 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     5701 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     5761 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     5821 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     5881 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     5941 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     6001 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     6061 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     6121 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     6181 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     6241 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     6301 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     6361 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     6421 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     6481 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6541 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6601 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     6661 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     6721 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     6781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7561 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
     7621 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
     7681 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
     7741 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
     7801 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
     7861 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
     7921 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
     7981 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
     8041 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
     8101 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
     8161 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
     8221 tccttttccc gttccacatc ataggttgtc cctttatacc ggctgtccgt catttttaaa
     8281 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     8341 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     8401 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     8461 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     8521 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     8581 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     8641 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     8701 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     8761 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     8821 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     8881 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     8941 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     9001 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     9061 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     9121 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc
     9181 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     9241 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     9301 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     9361 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc
     9421 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     9481 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     9541 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     9601 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     9661 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     9721 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     9781 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa
     9841 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     9901 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg
     9961 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
    10021 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
    10081 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
    10141 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggat ctgcgaaagc
    10201 tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa
    10261 atgaacttcc ttatatagag gaaggtcttg cgaaggatag tgggattgtg cgtcatccct
    10321 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
    10381 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
    10441 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
    10501 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
    10561 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
    10621 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttatcacatc aatccacttg
    10681 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
    10741 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
    10801 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
    10861 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
    10921 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
    10981 caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg
    11041 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    11101 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    11161 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    11221 catgattac
//