| 出品公司: | Novagen |
|---|---|
| 载体名称: | pIEx/Bac-3 |
| 质粒类型: | 昆虫细胞表达载体；蛋白纯化 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | IE-1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6802 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | N-His tag, C-Strep tagII |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | -- |
| 备注: | 具有两套标签，可以采用两套纯化方案进行蛋白纯化。 |
| 产品目录号: | #71726 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 杆状病毒 |
买家导航
pIEx/Bac-3载体 pIEx-Bac-3质粒基本信息
pIEx/Bac-3质粒图谱 pIEx-Bac-3载体图谱和pIEx/Bac-3载体序列 pIEx-Bac-3质粒序列多克隆位点信息
pIEx/Bac-3质粒 pIEx-Bac-3载体简介
The pIEx/Bac-3 vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in
Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV)
enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV
p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions
to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His•Tag® coding sequence with 10
histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal
thrombin protease cleavage site and 8 aa Strep•Tag® II coding sequence. pIEx/Bac-3 is also available as a 3C/LIC vector. The
3C/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for
amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.
pIEx/Bac-3质粒序列 pIEx-Bac-3载体序列
LOCUS pIEx/Bac-3 6802 bp DNA circular 17-OCT-2006
DEFINITION pIEx/BAC/p10 10xHis/3cLIC/Tb/Strep.
SOURCE
ORGANISM
FEATURES Location/Qualifiers
misc_recomb complement(2922..4340)
/dnas_title="1629"
/vntifkey="86"
/label=1629
rep_origin 6277..6732
/dnas_title="f1 origin"
/vntifkey="33"
/label=f1 origin
CDS complement(5288..6145)
/dnas_title="Ap"
/vntifkey="4"
/label=Ap
rep_origin complement(4527..4527)
/dnas_title="origin"
/vntifkey="33"
/label=origin
misc_recomb 4..453
/dnas_title="lef-2"
/vntifkey="86"
/label=lef-2
misc_recomb complement(491..1096)
/dnas_title="ORF 603"
/vntifkey="86"
/label=ORF 603
rep_origin 4360..5119
/dnas_title="pUC Ori"
/vntifkey="33"
/label=pUC Ori
enhancer 1196..1677
/dnas_title="hr5"
/vntifkey="9"
/label=hr5
polyA_signal 2748..2753
/dnas_title="PolyA signal"
/vntifkey="25"
/label=PolyA signal
5'UTR 2229..2278
/dnas_title="ie1 5'-UTR"
/vntifkey="52"
/label=ie1 5'-UTR
promoter 2279..2391
/dnas_title="p10 promoter + 5'UTR"
/vntifkey="29"
/label=p10 promoter + 5'UTR
misc_feature 2277..2280
/dnas_title="Bsa I jct"
/vntifkey="21"
/label=Bsa I jct
promoter 1682..2228
/dnas_title="ie1 promoter"
/vntifkey="29"
/label=ie1 promoter
terminator 2571..2877
/dnas_title="ie1 Terminator"
/vntifkey="43"
/label=ie1 Terminator
protein_bind 2529..2552
/dnas_title="Strep-Tag II"
/vntifkey="31"
/label=Strep-Tag II
protein_bind 2436..2458
/dnas_title="3c"
/vntifkey="31"
/label=3c
misc_feature 2505..2521
/dnas_title="Thrombin site"
/vntifkey="21"
/label=Thrombin site
misc_binding 2400..2429
/dnas_title="10x His-Tag"
/vntifkey="20"
/label=10x His-Tag
BASE COUNT 1822 a 1464 c 1508 g 2008 t
ORIGIN
1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tccatggcac
2401 atcaccacca tcatcaccat caccaccacg gtgcacttga agtcctcttt cagggacccg
2461 ggtaccagga tcctgtacaa gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta
2521 gttccgcttg gagccacccg cagttcgaaa agtaagtgat taacctcagg ttatacatat
2581 attttgaatt taattaatta tacatatatt ttatattatt tttgtctttt attatcgagg
2641 ggccgttgtt ggtgtggggt tttgcataga aataacaatg ggagttggcg acgttgctgc
2701 gccaacacca cctcccttcc ctcctttcat catgtatctg tagataaaat aaaatattaa
2761 acctaaaaac aagaccgcgc ctatcaacaa aatgataggc attaacttgc cgctgacgct
2821 gtcactaacg ttggacgatt tgccgactaa accttcatcg cccagtaacc aatctaggta
2881 gctgagcgca tgcaagctga tccgggttat tagtacattt attaagcgct agattctgtg
2941 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct
3001 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta
3061 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt
3121 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc
3181 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa
3241 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg
3301 tacaattgac tcgacgtaaa cacgttaaat agagcttgga catatttaac atcgggcgtg
3361 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc
3421 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg
3481 atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt
3541 ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc
3601 ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa
3661 tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc
3721 ggtgatgcag acggcggttt aggctcaaat gtctctttag gcaacacagt cggcacctca
3781 actattgtac tggtttcggg cgccgttttt ggtttgaccg gtctgagacg agtgcgattt
3841 ttttcgtttc taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc agcgggttga
3901 ggttccgtcg gcattggtgg agcgggcggc aattcagaca tcgatggtgg tggtggtggt
3961 ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc cggtataatt
4021 tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc cgctggctgc
4081 acaacggaag gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc aatattataa
4141 ttggaataca aatcgtaaaa atctgctata agcattgtaa tttcgctatc gtttaccgtg
4201 ccgatattta acaaccgctc aatgtaagca attgtattgt aaagagattg tctcaagctc
4261 ggatcgatcc cgcacgccga taacaagcct tttcattttt actacagcat tgtagtggcg
4321 agacacttcg ctgtcgtcga ggtttaaacg cttcctcgct cactgactcg ctgcgctcgg
4381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
4441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
4501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
4561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
4621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
4681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
4741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
4801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
4861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
4921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
4981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
5041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
5101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
5161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
5221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
5281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
5341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
5401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
5461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
5521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
5581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
5641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
5701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
5761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
5821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
5881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
5941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
6001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
6061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
6121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
6181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
6241 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
6301 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
6361 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
6421 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
6481 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
6541 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
6601 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
6661 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
6721 cgtttacaat ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
6781 gggcctcttc gctattacgc ca
//
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pIEx/Bac-3质粒 pIEx-Bac-3载体应用举例
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