| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.72 ; pGL4.72[hRlucCP] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 3704 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRlucCP |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.72[hRlucCP]载体是信号通路报告载体；无启动子；含海肾 荧光素酶报告基因hRluc。 |
| 产品目录号: | E6901 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.72[hRlucCP]载体pGL4.72质粒基本信息
pGL4.72[hRlucCP]质粒图谱pGL4.72载体图谱和pGL4.72[hRlucCP]载体序列pGL4.72质粒序列多克隆位点信息
pGL4.72[hRlucCP]质粒pGL4.72载体简介
pGL4.72载体简介 The pGL4.72[hRlucCP] Vector encodes the luciferase reporter gene hRlucCP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.72[hRlucCP] Vector has no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice. The hRlucCP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by hRlucCP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.
pGL4.72[hRlucCP]质粒序列pGL4.72载体序列
LOCUS AY738228 3704 bp DNA circular SYN 08-NOV-2004
DEFINITION Renilla luciferase reporter vector pGL4.72[hRlucCP], complete
sequence.
ACCESSION AY738228
VERSION AY738228.1 GI:55535636
KEYWORDS .
SOURCE Renilla luciferase reporter vector pGL4.72[hRlucCP]
ORGANISM Renilla luciferase reporter vector pGL4.72[hRlucCP]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3704)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3704)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2004) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..3704
/organism="Renilla luciferase reporter vector
pGL4.72[hRlucCP]"
/mol_type="other DNA"
/db_xref="taxon:296665"
misc_feature 1..70
/note="multiple cloning site region"
CDS 100..1212
/codon_start=1
/transl_table=11
/product="luciferase hRlucCP"
/protein_id="AAV52881.1"
/db_xref="GI:55535637"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSACKNWFSSLSHFVIHLNSHGF
PPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
misc_feature 1249..1470
/note="SV40 late poly(A) region"
primer_bind 1538..1557
/note="reporter vector primer 4 (RVPrimer4)"
rep_origin 1795
/note="ColE1-derived"
gene complement(2586..3446)
/gene="ampR"
CDS complement(2586..3446)
/gene="ampR"
/note="AmpR"
/codon_start=1
/transl_table=11
/product="synthetic beta-lactamase"
/protein_id="AAV52882.1"
/db_xref="GI:55535638"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3551..3704
/regulatory_class="polyA_signal_sequence"
/note="synthetic; transcriptional pause site"
primer_bind complement(3653..3672)
/note="reporter vector primer 3 (RVPrimer3)"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agaattctgc ttgcaagaac tggttcagta gcttaagcca ctttgtgatc
1081 caccttaaca gccacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
1141 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
1201 atcaacgtct aactgcagtc tagagtcggg gcggccggcc gcttcgagca gacatgataa
1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
1441 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc
1501 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
1561 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc
1621 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
1681 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
1741 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
1801 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
1861 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
1921 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
1981 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
2041 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
2101 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
2161 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
2221 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
2281 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
2341 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
2401 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
2461 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
2521 aatctaaagt atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc
2581 agtggttacc agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg
2641 tccatagtgg cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca
2701 ggccccagcg cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca
2761 atgaaccagc cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc
2821 atccagtcta tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc
2881 cgaagagttg tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct
2941 tcgttcaact ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga
3001 aatgcagtca gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg
3061 tcgctcatgg taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc
3121 ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca
3181 agctgctctt gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa
3241 gtgctcatca tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg
3301 agatccagtt cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc
3361 accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt
3421 gcgacacgaa aatgttggat gctcatactc gtcctttttc aatattattg aagcatttat
3481 cagggttact agtacgtctc tcaaggataa gtaagtaata ttaaggtacg ggaggtattg
3541 gacaggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
3601 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
3661 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctct
//
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