| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.71 ; pGL4.71[hRlucP] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 3653 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRlucP |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.71[hRlucP]载体是信号通路报告载体；无启动子；含海肾荧光素酶报告基因hRlucP。 |
| 产品目录号: | E6891 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.71[hRlucP]载体pGL4.71质粒基本信息
pGL4.71[hRlucP]质粒图谱pGL4.71载体图谱和pGL4.71[hRlucP]载体序列pGL4.71质粒序列多克隆位点信息
pGL4.71[hRlucP]质粒pGL4.71载体简介
pGL4.71载体简介 The pGL4.71[hRlucP] Vector encodes the luciferase reporter gene hRlucP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.71[hRlucP] Vector has no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice. The hRlucP luciferase reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by hRlucP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.
pGL4.71[hRlucP]质粒序列pGL4.71载体序列
LOCUS AY738227 3653 bp DNA circular SYN 08-NOV-2004
DEFINITION Renilla luciferase reporter vector pGL4.71[hRlucP], complete
sequence.
ACCESSION AY738227
VERSION AY738227.1 GI:55535633
KEYWORDS .
SOURCE Renilla luciferase reporter vector pGL4.71[hRlucP]
ORGANISM Renilla luciferase reporter vector pGL4.71[hRlucP]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3653)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3653)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2004) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..3653
/organism="Renilla luciferase reporter vector
pGL4.71[hRlucP]"
/mol_type="other DNA"
/db_xref="taxon:296664"
misc_feature 1..70
/note="multiple cloning site region"
CDS 100..1158
/codon_start=1
/transl_table=11
/product="luciferase hRlucP"
/protein_id="AAV52879.1"
/db_xref="GI:55535634"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSHGFPPEVEEQAAGTLPMSCAQ
ESGMDRHPAACASARINV"
misc_feature 1198..1419
/note="SV40 late poly(A) region"
primer_bind 1487..1506
/note="reporter vector primer 4 (RVPrimer4)"
rep_origin 1744
/note="ColE1-derived"
gene complement(2535..3395)
/gene="ampR"
CDS complement(2535..3395)
/gene="ampR"
/note="AmpR"
/codon_start=1
/transl_table=11
/product="synthetic beta-lactamase"
/protein_id="AAV52880.1"
/db_xref="GI:55535635"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3500..3653
/regulatory_class="polyA_signal_sequence"
/note="synthetic; transcriptional pause site"
primer_bind complement(3602..3621)
/note="reporter vector primer 3 (RVPrimer3)"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc
1081 ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc
1141 gccaggatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
1201 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
1261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
1321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
1381 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
1441 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
1501 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
1561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
1621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
1681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
1741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
1801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
1861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
1921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
1981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
2041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
2101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
2161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
2221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
2281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
2341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
2401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
2461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta
2521 aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta
2581 tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc
2641 ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat
2701 ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg
2761 tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg
2821 agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc
2881 ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata
2941 ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc
3001 gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc
3061 gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata
3121 cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt
3181 actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg
3241 ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct
3301 tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag
3361 ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga
3421 agcatttatc agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg
3481 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
3541 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
3601 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct
//
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pGL4.71[hRlucP]质粒pGL4.71载体应用举例
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