| 质粒名称: | pAAV-EF1a-FLEX-GTB |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26197 |
| 存储实验室: | Edward Callaway |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-double floxed-EYFP-WPRE-pA |
| 载体出品公司: | Karl Deisseroth Lab |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5300 bp |
| 完整质粒大小: | 8550 bp |
| 载体类型: | AAV, Cre/Lox ; Adeno-associated virus |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein |
| 物种来源: | 人源 |
| 插入大小: | 3250 bp |
| 基因又名: | B19G, RG；tv-a；eGFP |
| 基因突变: | TVA, rabies glycoprotein are mammalian codon optimized |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | EF1a_fwd_primer |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-EF1a-FLEX-GTB was a gift from Edward Callaway (爱迪基因 plasmid # 26197) |
| 参考文献部分: | Genetic dissection of an amygdala microcircuit that gates conditioned fear. Haubensak W, Kunwar PS, Cai H, Ciocchi S, Wall NR, Ponnusamy R, Biag J, Dong HW, Deisseroth K, Callaway EM, Fanselow MS, Luthi A, Anderson DJ. Nature. 2010 Nov 11. 468(7321):270-6. 10.1038/nature09553 PubMed 21068836 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAV-EF1a-FLEX-GTB 8565 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-EF1a-FLEX-GTB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8565)
AUTHORS Haubensak W, Kunwar PS, Cai H, Ciocchi S, Wall NR, Ponnusamy R, Biag
J, Dong HW, Deisseroth K, Callaway EM, Fanselow MS, Luthi A,
Anderson DJ
TITLE Genetic dissection of an amygdala microcircuit that gates
conditioned fear.
JOURNAL Nature. 2010 Nov 11. 468(7321):270-6.
PUBMED 21068836
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-EF1a-FLEX-GTB|
FEATURES Location/Qualifiers
source 1..8565
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 12..141
/label=AAV2 ITR
promoter 242..1419
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 473..1410
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1367..1387
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 1441..1450
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
protein_bind complement(1453..1486)
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites."
protein_bind complement(1549..1582)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS complement(3173..3232)
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
CDS complement(3722..3775)
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS complement(3791..4504)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(3833..3854)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 4182..4201
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4441..4462
/label=EGFP-N
/note="EGFP, reverse primer"
protein_bind 4513..4546
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites."
protein_bind 4617..4650
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 4692..5280
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4745..4765)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5163..5174)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5312..5788
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(5511..5530)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 5828..5968
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 5828..5957
/label=AAV2 ITR
rep_origin 6043..6498
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6130..6149)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6340..6361
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6515..6534)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6634..6656
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6694..6712)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6780..6884
/gene="bla"
/label=AmpR promoter
CDS 6885..7745
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7103..7122)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7916..8504
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8405..8424
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagcc
1441 gccaccatgg ccataacttc gtataaagta tcctatacga agttatatca aaataggaag
1501 accaatgctt caccatcgac ccgaattgcc aagcatcacc atcgacccat aacttcgtat
1561 aatgtatgct atacgaagtt atacttcaac tagttcacag tctggtctcg ccgccgctct
1621 tgtggctctc ccagctgctg atgatcttgc cgctctgggg ggtcacgctc acctctctgc
1681 cggtgcctct caggttgtgc tgggtgggct cgcttctgtt cactcttctg cagcaggtca
1741 tcaggaagat gatcagcatc agggcggtca gggcgccggc gctcagcagc acgtacttgc
1801 cccagttggg caggcccagg tccacgccgc tcacctggtt gtgcacgtcg ggcaggtgca
1861 cctccacgaa gtcctcggcc tcgtcgccgt ccttgaacac ggtgctgggg tcggccaggg
1921 ggtgcaccag ggggatcacg ctgctctcca gcagctccat gtgctgctgc agcaggctgc
1981 tctgcatctc ggggatcagc acgttgccgt cggggcccag gatgatgccg ttgaagaaca
2041 cgccgttcac gtgggggtgg catctgccgc ccactctcag gcagcccttg ctgggcagga
2101 tctcgttcca ggttctcacg ctcttgtagt gggcgtcggc ctccatcagg gtcttgttga
2161 agatggtgta ggccttgccg aagccgggca ccagctttct caggtggctc agtcttctga
2221 agctcacgct cttggtggtc atgatgctct ccagggcgtc caggcactcc tctctctttc
2281 tcaccagctc ctccaccacc aggtgctcga tctcgtcgct tctgaagtcg tgcaggttca
2341 ccagcttgtc gggggggcac cacttggtct cgttgctggt ctgcatgctc acccaggtgc
2401 cgtccatcag tctcaggccc agcacgccgc acagcttcag cttgcaggcg cccttcaggc
2461 tcttgtacag gcctctctcg tccacgaagc cgcaggtctc gctgcccttg ctggctctct
2521 tgcctctgct gttggtgaag atgtcgcagc tcatgcccag tctggggttc tcgggcatcc
2581 agatggtgta gtcgtggttg gtgctgcagt aggtgctgct cacggccacg ccgctgcact
2641 tgccgctggg gaacactctg ctgtgcaggc ttctgtcgta ggggtccagg tcggccacgc
2701 tggggctgat gatcaccagg ctctccttgg tggtcttcac ggttctcagc catctgtagt
2761 cggggtaggg gttgtgcagg ctctcctcgt atctggggtc gccggccatc ttccagttgt
2821 aggcggctct gcaggcgtcg ggggtgggtc tgaagtgctt tctcttgaag gtggtggtca
2881 cgtagcccac gaagttggtg taggtctcgg cctcggtcac cacgccggtg caggtgaagc
2941 cgttcacctt gatggccagg atgtagccca ccttcagctc catgtagctg aagccgctca
3001 ggttggtgca gccctcgtcc tccaccacca ggttgttggg gcagctcagg tggtggatgt
3061 cgatggggct ccaggggccc agcttgtcgg ggatggtgta gatggggaac ttgccgaagc
3121 acagggggaa caccagcagg ggcacgaaca gcagggcctg gggcactccg gaaggaccgg
3181 ggttactttc aacatcgcca gcgagtttca acaaagcgta gttagtacat tgaccactac
3241 cgctagcgga gaacaagtct gcctggctct tgtcaggcac cagcagctcc ttggatgcgc
3301 tttcaagact gaagatgtca gacctgcttt ttgctttgga cttcccccat gcagcgatac
3361 cacccacagc taccaggcag cacaggagca ctgcagtgat cagcatccac atgcggccgt
3421 gattcctggc tggcagagca cgtccaggag caggggcagt gggagcctct gtgccgttgt
3481 ccgtgggcac cgcggggatc gcgctggtcc cgcagcccca ctcgtcccgc ccgtcgtcgc
3541 agtcggggtg tccgtcgcac agccagtcct gcgggtaaca ctccccgtgg gcaccgggcg
3601 gctccgagca gcggaactga ccgggggggc aacgggacaa agaaccgtta ccggacccgt
3661 taccggtcac gttaccgggc agcagcagca gcagcagcgc gggcagcagc cgcgcatcga
3721 ttgggccagg attctcctcg acgtcaccgc atgttagcag acttcctctg ccctctccac
3781 taccaagctt cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
3841 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
3901 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
3961 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
4021 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
4081 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
4141 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
4201 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
4261 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
4321 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
4381 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
4441 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
4501 tcacctcgac gcataacttc gtataggata ctttatacga agttatcttt gggattcttc
4561 ctattttgat ccaagcatca ccatcgaccc tctagtccag atctcaccat cgacccataa
4621 cttcgtatag catacattat acgaagttat gtccctcgaa gaggttcgaa ttcgatatca
4681 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
4741 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
4801 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
4861 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
4921 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
4981 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
5041 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
5101 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
5161 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
5221 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
5281 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
5341 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
5401 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
5461 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
5521 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
5581 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
5641 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
5701 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
5761 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
5821 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
5881 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
5941 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
6001 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
6061 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
6121 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
6181 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
6241 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
6301 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
6361 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
6421 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
6481 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
6541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
6601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
6661 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
6721 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
6781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
6841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
6901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
6961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
7021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
7081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
7141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
7201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
7261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
7321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
7381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
7441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
7501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
7561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
7621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
7681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
7741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
7801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
7861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
7921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
7981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
8041 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
8101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
8161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
8221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
8281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
8341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
8401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
8461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
8521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
