| 出品公司: | -- |
|---|---|
| 载体名称: | pcDNA3.1(+)/CAT |
| 质粒类型: | 哺乳动物表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 6217 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin（新霉素) |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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pcDNA3.1(+)/CAT载体质粒基本信息
pcDNA3.1(+)/CAT质粒图谱载体图谱和pcDNA3.1(+)/CAT载体序列质粒序列多克隆位点信息
CAT 载体图谱.png)
pcDNA3.1(+)/CAT质粒载体简介
pcDNA3.1(+)/CAT质粒序列载体序列
LOCUS pcDNA3.1(+)/CAT 6217 bp DNA circular SYN 12-JUL-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6217) AUTHORS Biofeng TITLE Direct Submission JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2 https://www.biofeng.com FEATURES Location/Qualifiers source 1..6217 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1027..1686 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" polyA_signal 1817..2041 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2087..2515 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2529..2858 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2709..2844 /label=SV40 ori /note="SV40 origin of replication" CDS 2925..3719 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3893..4014 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4063..4079) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 4087..4103 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4111..4141) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4156..4177 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4465..5050) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5221..6081) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6082..6186) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 961 agatatccag cacagtggcg gccgctcgaa gcttcgacga gattttcagg agctaaggaa 1021 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt 1081 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag 1141 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc 1201 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa 1261 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa 1321 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac 1381 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt 1441 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta 1501 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg 1561 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc 1621 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg 1681 gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac gcctgaataa 1741 gtgataataa gcggatgaat ggcagaaatt cgtcgaagct ctagagggcc cgtttaaacc 1801 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 1861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 1921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 1981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 2041 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 2101 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 2161 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 2221 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 2281 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 2341 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 2401 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 2461 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 2521 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 2581 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 2641 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 2701 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 2761 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 2821 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 2881 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 2941 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 3001 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 3061 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 3121 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 3181 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 3241 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 3301 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 3361 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 3421 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 3481 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 3541 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 3601 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 3661 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 3721 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt 3781 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 3841 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 3901 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 3961 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 4021 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 4081 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 4141 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 4201 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 4261 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 4321 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 4381 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 4441 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 4501 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 4561 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 4621 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 4681 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 4741 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 4801 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 4861 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 4921 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 4981 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 5041 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 5101 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 5161 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 5221 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 5281 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 5341 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 5401 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 5461 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 5521 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 5581 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 5641 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 5701 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 5761 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 5821 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 5881 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 5941 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 6001 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 6061 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 6121 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 6181 tccgcgcaca tttccccgaa aagtgccacc tgacgtc //
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pcDNA3.1(+)/CAT质粒载体应用举例
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