| 质粒名称: | pPB-AMBRA1-3xFLAG |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 174159 |
| 存储实验室: | Julien Sage |
|
载体骨架基本信息 |
|
| 载体名称: | VectorBuilder pPB |
| 载体出品公司: | VectorBuilder |
| 完整质粒大小: | 10964 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | AMBRA1 |
| 物种来源: | H. sapiens (human) |
| 启动子: | EF1A |
| 标签/融合蛋白: | 3xFLAG (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pPB-AMBRA1-3xFLAG was a gift from Julien Sage (Addgene plasmid # 174159 ; http://n2t.net/addgene:174159 ; RRID:Addgene_174159) |
| 参考文献部分: | The AMBRA1 E3 ligase adaptor regulates the stability of cyclin D. Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R, Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP, Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ, McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK, Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J. Nature. 2021 Apr;592(7856):794-798. doi: 10.1038/s41586-021-03474-7. Epub 2021 Apr 14. 10.1038/s41586-021-03474-7 PubMed 33854239 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pPB-AMBRA1-3xFLAG 10964 bp ds-DNA circular SYN 10-SEP-2021
DEFINITION PiggyBac vector to stably express 3xFLAG-tagged AMBRA1.
ACCESSION .
VERSION .
KEYWORDS pPB-AMBRA1-3xFLAG
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10964)
AUTHORS Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R,
Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP,
Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ,
McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK,
Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J
TITLE The AMBRA1 E3 ligase adaptor regulates the stability of cyclin D.
JOURNAL Nature. 2021 Apr;592(7856):794-798. doi: 10.1038/s41586-021-03474-7.
Epub 2021 Apr 14.
PUBMED 33854239
REFERENCE 2 (bases 1 to 10964)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 10, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10964
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 5..39
/label=piggyBac left (5') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
protein_bind 316..336
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
promoter 337..1515
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 568..1506
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1463..1483
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
protein_bind 1515..1539
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 5188..5253
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
protein_bind complement(5257..5281)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(5359..5378)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 5424..5479
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(5478..5497)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
enhancer 5834..6213
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 6214..6417
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 6368..6388
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 6450..7244
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6504..6523)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7114..7133
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(7282..7299)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7288..7512
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 7862..7924
/label=piggyBac right (3') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
primer_bind complement(7990..8009)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7991..8009)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8016..8033)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8016..8032)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8025..8047)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 8174..8629
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8261..8280)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8471..8492
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 8655..8759
/gene="bla"
/label=AmpR promoter
CDS 8760..9620
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8978..8997)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9791..10379
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10280..10299
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 10533..10550
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 10667..10688
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 10703..10733
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10741..10757
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 10746..10768
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 10765..10781
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 10765..10781
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 10802..10820
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
301 tgttatagat atcatcaact ttgtatagaa aagttgggct ccggtgcccg tcagtgggca
361 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
421 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
481 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
541 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
601 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
661 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
721 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
781 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
841 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
901 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
961 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
1021 gggtagtctc aagctggccg gcctgctctg gtgcctggtc tcgcgccgcc gtgtatcgcc
1081 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
1141 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
1201 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
1261 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
1321 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
1381 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
1441 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
1501 tttcaggtgt cgtgacaagt ttgtacaaaa aagcaggctg ccaccatgaa ggttgtccca
1561 gaaaagaatg ctgtccggat actctggggg cgagaacggg gtgctcgggc catgggagct
1621 cagcggcttc tgcaggagct ggtagaggat aaaacccggt ggatgaaatg ggagggcaag
1681 agagtagaac tgccggattc accacgctct accttcttat tggccttcag cccagacagg
1741 actctcttag ccagcaccca tgtgaaccat aatatctata ttacggaggt gaagactggc
1801 aagtgtgttc attccctgat tggacaccgc cgtactccat ggtgtgtcac ttttcatccc
1861 accatctcag gccttattgc ttctggctgc ctagatgggg aggttaggat ttgggattta
1921 cacggtggca gtgaaagctg gttcacagat agcaacaatg ccattgcctc cctggctttc
1981 caccctacgg ctcagctcct gctgattgcc actgccaatg agatccactt ctgggactgg
2041 agtcgacggg aaccctttgc tgtggtgaag acagctagtg agatggaacg ggtccgtctg
2101 gtgagatttg atccacttgg acactactta ctcacagcaa ttgttaaccc ctctaatcaa
2161 cagggtgatg acgaaccaga gatccccata gatggaacag aattatccca ctaccgtcag
2221 cgtgccctcc tgcaatcaca gccagttcgc cggacgcctc tcctccacaa tttcctgcac
2281 atgctgtcct cccgctcttc tggcatccag accgagccct tccatccccc ggagcaggcc
2341 tcgtcaacgc agcaggacca gggcctcctg aaccggccgt ctgccttcag tacagtccag
2401 agcagcactg ccggcaacac gctccgcaac ctcagtctgg gtcctacccg ccgctctttg
2461 ggagggcctc tgtctagcca cccttctagg tatcaccgag aaatagctcc tgggttgaca
2521 ggatctgagt ggacccggac agtactcagt ctgaactccc gctctgaggc ggaatccatg
2581 cccccgccca gaaccagtgc ctcttcggtg agtttgctgt ctgtgctgag acagcaggaa
2641 ggtggctctc aggcatctgt gtacacttca gccacagaag ggaggggttt tccggcatca
2701 gggttggcaa ctgagtcaga tggagggaat ggctccagcc aaaacaactc gggcagcatt
2761 cgccatgagc ttcagtgtga cctgagacgc ttctttctgg agtatgaccg gcttcaggag
2821 ctggatcaga gcctgagtgg ggaagctccc cagacccaac aggcccagga aatgctcaac
2881 aataacattg aatctgagag gccaggccct tcccaccagc ccaccccaca cagcagtgag
2941 aacaactcca acctgtcccg tggccacctg aatcgctgtc gtgcttgcca caatctcctg
3001 accttcaaca acgataccct gcgctgggaa agaaccacac ctaactactc ctctggcgag
3061 gctagttcct cttggcaggt ccccagctcc tttgagagtg tgccatcaag tggcagccag
3121 ttgccacctc tcgagcggac tgagggccaa acgcccagct ccagcaggct ggagttgagc
3181 agctctgcta gtccgcagga ggagaggact gtgggggtgg cctttaacca ggagacaggc
3241 cactgggaaa gaatttacac ccagtccagc agatctggaa ctgtgtcaca ggaggcctta
3301 catcaggata tgcctgagga gagctctgag gaggattcac tcaggaggag gctgctggaa
3361 tcttccctca tttcattatc ccgttatgat ggagcaggat ccagagagca cccaatttac
3421 ccagacccag cgagattatc tcctgctgca tactacgccc agaggatgat ccagtatctc
3481 tcacggagag acagtattcg ccagcgctcc atgcgctacc aacagaaccg tctccgttct
3541 tccacctcct cctcttcctc agacaaccag ggtccatcag tagagggaac cgacttggaa
3601 tttgaggact ttgaggacaa tggtgacaga tccaggcacc gagctccacg caatgcccgg
3661 atgtctgcac cttcgcttgg acgctttgtc ccaaggcgtt tcttgctgcc tgagtacttg
3721 ccttatgctg ggatttttca tgaacgtgga cagcctggct tggctactca ctcttctgtt
3781 aacagggtcc tggcaggggc agtgatcggt gatggacagt ctgctgtggc cagtaacatt
3841 gccaatacta cctaccggct ccagtggtgg gacttcacta agtttgacct ccctgaaatc
3901 agtaatgctt ccgtgaatgt gctggtgcag aactgcaaga tctacaatga tgccagctgt
3961 gacatttctg cagatggcca gctcctggca gctttcatcc ccagcagcca gaggggcttt
4021 cctgatgaag gcatcctggc agtgtactcc ctggcccccc ataacctggg cgaaatgctc
4081 tacaccaagc gatttggtcc caatgccatt tcggtgagcc tgtccccaat gggcagatat
4141 gtaatggtgg gcttggcctc acgaaggatc ctgctgcacc cctccacaga gcacatggtg
4201 gcccaggtct tcaggctgca acaggcccat ggtggagaga cctccatgag gagagttttc
4261 aacgtccttt atcccatgcc tgccgaccag cggagacatg tcagtatcaa ctctgcccgt
4321 tggctgcctg agccagggct tggcttggcc tatggtacta acaaaggaga cctggtgatc
4381 tgccgaccag aggccttaaa ctctggtgtt gagtactact gggaccagct gaacgagacg
4441 gtcttcactg tccattccaa cagcaggagc agcgagcggc ctggaaccag cagagccaca
4501 tggaggacag acagagacat ggggctgatg aatgccattg ggcttcagcc ccggaaccct
4561 gccacctcag tgacatctca gggcacccag actctggccc ttcagctgca gaatgccgaa
4621 acacagactg agagggaggt gccggagcca gggacagccg cctcaggtcc tggtgaaggt
4681 gagggttcag agtatggtgc cagtggagaa gatgcgctca gcaggatcca gaggctgatg
4741 gcggagggcg gcatgacagc cgtggtgcag cgggagcaga gcaccaccat ggcctccatg
4801 ggcggcttcg gcaacaacat catcgtcagc caccgcattc accgcagctc tcagacgggc
4861 actgaacctg gtgccgccca cacctcctca ccccagccct ccacctctcg gggactgctc
4921 ccagaggccg ggcaactggc agagcgaggc ctaagccccc ggacagcttc ctgggaccag
4981 cctggtaccc ctgggcggga gccaacccag ccaaccctgc cctcttcctc ccctgtcccc
5041 attcctgttt cccttcccag cgctgaggga ccaaccctcc actgcgagtt gaccaataac
5101 aaccaccttc tggatggtgg cagcagcagg ggggacgctg caggccctag gggagaacca
5161 cggaacaggg gcagcggcag cggcagcgac tacaaagacc atgacggtga ttataaagat
5221 catgatatcg attacaagga tgacgatgac aagtagaccc agctttcttg tacaaagtgg
5281 tgatcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
5341 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
5401 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
5461 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
5521 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
5581 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
5641 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
5701 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
5761 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
5821 caagcttcgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
5881 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
5941 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
6001 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
6061 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
6121 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
6181 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
6241 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
6301 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
6361 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
6421 tggctaacta gagaacccac tgcgccacca tgattgaaca agatggattg cacgcaggtt
6481 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
6541 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
6601 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
6661 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
6721 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
6781 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
6841 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
6901 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
6961 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
7021 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
7081 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
7141 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
7201 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgactcgag tctagagggc
7261 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
7321 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
7381 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
7441 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
7501 tgggctctat ggctcgagtt aattaacgag agcataatat tgatatgtgc caaagttgtt
7561 tctgactgac taataagtat aatttgtttc tattatgtat aggttaagct aattacttat
7621 tttataatac aacatgactg tttttaaagt acaaaataag tttatttttg taaaagagag
7681 aatgtttaaa agttttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata
7741 aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat
7801 aataaaactt aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac
7861 acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct
7921 agggttaaat aatagtttct aattttttta ttattcagcc tgctgtcgtg aataccgagc
7981 tccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
8041 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
8101 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
8161 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
8221 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
8281 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
8341 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
8401 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
8461 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
8521 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
8581 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
8641 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
8701 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
8761 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
8821 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
8881 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
8941 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
9001 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
9061 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
9121 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
9181 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
9241 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
9301 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
9361 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
9421 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
9481 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
9541 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
9601 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
9661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
9721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
9781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
9841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
9901 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
9961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
10021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
10081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
10141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
10201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
10261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
10321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
10381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
10441 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
10501 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
10561 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
10621 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
10681 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
10741 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg
10801 aaattaaccc tcactaaagg gaacaaaagc tggtacctcg cgcgacttgg tttgccattc
10861 tttagcgcgc gtcgcgtcac acagcttggc cacaatgtgg tttttgtcaa acgaagattc
10921 tatgacgtgt ttaaagttta ggtcgagtaa agcgcaaatc tttt
//
