| 质粒名称: | pMR-A-3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 138517 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV with a BR322 origin |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 5188 bp |
| 载体类型: | Synthetic Biology |
| 载体抗性: | Spectinomycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | T7-RNAP |
| 物种来源: | Other |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: |
pMR-A-3 was a gift from David Liu (Addgene plasmid # 138517 ; http://n2t.net/addgene:138517 ; RRID:Addgene_138517)
|
| 参考文献部分: |
Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR. Nat Biotechnol. 2020 Jul;38(7):883-891. doi: 10.1038/s41587-020-0453-z. Epub 2020 Mar 16. 10.1038/s41587-020-0453-z PubMed 32433547
|
买家导航
pMR-A-3载体质粒基本信息
pMR-A-3质粒图谱载体图谱和pMR-A-3载体序列质粒序列多克隆位点信息
pMR-A-3质粒载体简介
pMR-A-3质粒序列载体序列
LOCUS pMR-A-3 5188 bp DNA circular SYN 25-AUG-2024
DEFINITION P3a.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5188)
AUTHORS Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW,
Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR
TITLE Phage-assisted evolution of an adenine base editor with improved Cas
domain compatibility and activity.
JOURNAL Nat Biotechnol. 2020 Jul;38(7):883-891. doi:
10.1038/s41587-020-0453-z. Epub 2020 Mar 16.
PUBMED 32433547
REFERENCE 2 (bases 1 to 5188)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41587-020-0453-z"; journalName: "Nat Biotechnol"; date:
"2020-07"; volume: "38"; issue: "7"; pages: "883-891"
FEATURES Location/Qualifiers
source 1..5188
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 301..320
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 554..571
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 586..726
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(652..671)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 812..834
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(828..1019)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(4109..4900)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
promoter complement(4901..4972)
/gene="bla"
/label=AmpR promoter
rep_origin join(5000..5188,1..400)
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
61 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
121 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
181 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
241 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
301 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
361 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
421 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
481 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
541 aacgaccgag cgcagcgagt cagtgagcga ggaagcggat gagcgcctga tgcggtattt
601 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
661 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
721 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
781 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
841 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
901 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
961 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
1021 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag
1081 aggatgctca cgatacgggt tactgatgat gaacaagagg acatccggtc aaataaaacg
1141 aaaggctcag tcgaaagact gggcctttcg ttttgctgag gagacttagg gactctcaca
1201 gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc tggataactt
1261 tacgggcatg cataaggctc gtaggctata ttcagggaga ccacaacggt ttccctctac
1321 aaataatttt gtttaacttt tactagagtg ggaccctacc tgcaggtgca gtaaggagga
1381 aaaaaaaatg aacacgatta acatcgctaa gaacgacttc tctgacatcg aactggctgc
1441 tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc
1501 ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagtgata
1561 acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact
1621 ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa
1681 gcgcccgaca gccttccagt tcctgcaaga aatcaagccg gaagccgtag cgtacatcac
1741 cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc
1801 aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttaa
1861 agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta
1921 caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg
1981 cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga
2041 gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg
2101 tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc
2161 aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg
2221 gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg
2281 tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa
2341 agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc
2401 caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga
2461 agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa
2521 acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct
2581 tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta
2641 caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga
2701 tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta
2761 ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg
2821 catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga
2881 gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta
2941 cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg
3001 gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc
3061 ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt
3121 caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac
3181 cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg
3241 tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc
3301 ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc
3361 tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc
3421 taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg
3481 gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat
3541 tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga
3601 atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca
3661 gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat
3721 cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc
3781 acacgagaag tacggtatcg aatcttttgc actgattcac gactccttcg gtaccattcc
3841 ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc
3901 ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt
3961 ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc
4021 cgacttcgcg ttcgcctaat ggcactcctc agcaaatata atgaccctct tgataaccca
4081 agagggcatt ttttaatgcc catggcgttt atttgccgac taccttggtg atctcgcctt
4141 tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt
4201 gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
4261 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
4321 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
4381 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
4441 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
4501 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
4561 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
4621 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
4681 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
4741 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
4801 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
4861 atagttgagt cgatacttcg gcgatcaccg cttccctcat actcttcctt tttcaatatt
4921 attgaagcat ttatcagggt tattgtctca tgagcgtata catatttgaa tgtatttaga
4981 aaaataggcc aaatagggtt tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
5041 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccacctcttt
5101 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
5161 cgtagttagg ccaccacttc aagaactc
//
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