| 质粒名称: | pJMP1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 79873 |
| 存储实验室: | Carol Gross, Stanley Qia |
|
载体骨架基本信息 |
|
| 载体名称: | pAX01 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7781 bp |
| 完整质粒大小: | 12080 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCas9 |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 插入大小: | 4107 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pJMP1 was a gift from Carol Gross & Stanley Qi (Addgene plasmid # 79873 ; http://n2t.net/addgene:79873 ; RRID:Addgene_79873) |
| 参考文献部分: | A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA. Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. 10.1016/j.cell.2016.05.003 PubMed 27238023 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJMP1 12080 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Bacillus subtilis dCas9 expression vector; integrates into
lacA/ganA.
ACCESSION .
VERSION .
KEYWORDS pJMP1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12080)
AUTHORS Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins
JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS,
Brown ED, Qi LS, Huang KC, Gross CA
TITLE A Comprehensive, CRISPR-based Functional Analysis of Essential Genes
in Bacteria.
JOURNAL Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003.
Epub 2016 May 26.
PUBMED 27238023
REFERENCE 2 (bases 1 to 12080)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12080
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1087..1278
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind complement(1272..1294)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 1380..1520
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 1435..1454
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(1535..1552)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1706..2294)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1786..1805)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2465..3325)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3088..3107
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3326..3430)
/gene="bla"
/label=AmpR promoter
terminator 4074..4108
/label=lambda t0 terminator
/note="minimal transcription terminator from phage lambda
(Scholtissek and Grosse, 1987)"
CDS 5580..9686
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
terminator 9710..9781
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 9797..9824
/label=T7Te terminator
/note="phage T7 early transcription terminator"
promoter complement(9899..10003)
/gene="bla"
/label=AmpR promoter
terminator 11289..11375
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 11394..11421
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 11501..11535
/label=lambda t0 terminator
/note="minimal transcription terminator from phage lambda
(Scholtissek and Grosse, 1987)"
ORIGIN
1 atcctgatat tgtctgcatt tgcgccggag caaaccaaaa acctggtgag acacgccttg
61 aattagtaga aaagaacttg aagattttca aaggcatcgt tagtgaagtc atggcgagcg
121 gatttgacgg cattttctta gtcgcgacgc gaggctggat ggccttcccc attatgattc
181 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
241 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
301 tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
361 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
421 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
481 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
541 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
601 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
661 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
721 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
781 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
841 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
901 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
961 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
1021 cctctctcgt ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg
1081 catcaagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
1141 acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt
1201 gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat
1261 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
1321 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
1381 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
1441 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
1501 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
1561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
1621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
1681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
1741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
1801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
1861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
1921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
1981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
2041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
2101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
2161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
2221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
2281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
2341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
2401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
2461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
2521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
2581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
2641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
2701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
2761 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
2821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
2881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
2941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
3001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
3061 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
3121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
3181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
3241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
3301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
3361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
3421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
3481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaatgatggg
3541 ctaatgtgtg tttacgacaa ttctcacttc atacttttcc atcgtcaggt cgcctgacaa
3601 tatgtctcct gtcattatgt ccttcacact ctgatcaaac gtgaccagct gtttttcttc
3661 cgtgaaattc atgacaaaaa tataatcatt gtcctgatcc tgcctcgctt gtacggagac
3721 gccttttccg tgccgaaccg gaaaaactgg agagagagac aggtctgtga tcagaccctc
3781 atagaaatca cgctgaaatt gatcctccaa acgcgcgccg ataaaatacg ccttgccctg
3841 ctgatactca tggcttgtga ccgctggcgt gcgcgcataa aaatcttctt gatacaccgc
3901 ttccactgaa gctgtcttta catcaatcac ggttgcataa tccttcattt catatatttg
3961 gctgcggtag ctgacagcgt ttcgatcctt cggatacagg gtgtccgttt caagaggctc
4021 aactccaaat atagcttgaa atcgatatct ctgcagtcgc gatgattaat taattcagaa
4081 cgctcggttg ccgccgggcg ttttttatgc agcaatggca agaacgtccc ggggagctcc
4141 taacttatag gggtaacact taaaaaagaa tcaataacga tagaaaccgc tcctaaagca
4201 ggtgcatttt ttcctaacga agaaggcaat agttcacatt tattgtctaa atgagaatgg
4261 actctagaag aaacttcgtt tttaatcgta tttaaaacaa tgggatgaga ttcaattata
4321 tgatttctca agataacagc ttctatatca aatgtattaa ggatattggt taatccaatt
4381 ccgatataaa agccaaagtt ttgaagtgca tttaacattt ctacatcatt tttatttgcg
4441 cgttccacaa tctcttttcg agaaatattc ttttcttctt tagagagcga agccagtaac
4501 gctttttcag aagcatataa ttcccaacag cctcgatttc cacagctgca tttgggtcca
4561 ttaaaatcta tcgtcatatg acccatttcc ccagaaaaac cctgaacacc tttatacaat
4621 tcgttgttaa taacaagtcc agttccaatt ccgatattaa tactgatgta aacgatgttt
4681 tcatagtttt ttgtcatacc aaatactttt tcaccgtatg ctcctgcatt agcttcattt
4741 tcaacaaaaa ccggaacatt aaactcactc tcaattaaaa actgcaaatc tttgatattc
4801 caatttaagt taggcatgaa aataatttgc tgatgacgat ctacaaggcc tggaacacaa
4861 attcctattc cgactagacc ataaggggac tcaggcatat gggttacaaa accatgaata
4921 agtgcaaata aaatctcttt tacttcacta gcggaagaac tagacaagtc agaagtcttc
4981 tcgagaataa tatttccttc taagtcggtt agaattccgt taagatagtc gactcctata
5041 tcaataccaa tcgagtagcc tgcattctta ttaaaaacaa gcattacagg tcttctgccg
5101 cctctagatt gccctgcccc aatttcaaaa ataaaatctt tttcaagcag tgtatttact
5161 tgagaggaga cagtagactt gtttaatcct gtaatctcag agagagttgc cctggagaca
5221 ggggagttct tcaaaatttc atctaatatt aatttttgat tcattttttt tactaaagct
5281 tgatctgcaa tttgaataat aaccactcct ttgtttatcc accgaactaa gttggtgttt
5341 tttgaagctt gaattagata tttaaaagta tcatatctaa tattataact aaattttcta
5401 aaaaaaacat tgaaataaac atttattttg tatatgatga gataaagtta gtttattgga
5461 taaacaaact aactcaatta agatagttga tggataaact tgttcactta aatcaaaggg
5521 ggaaatgaca aatggtccaa actagtgata tctaaaaatc aaagggggaa atgggatcca
5581 tggataagaa atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga
5641 tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc
5701 acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag
5761 cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt
5821 atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac
5881 ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa
5941 atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa
6001 aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata
6061 tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg
6121 tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta
6181 ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac
6241 gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc
6301 tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag
6361 atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc
6421 aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt
6481 tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa
6541 tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac
6601 aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag
6661 gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag
6721 aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca
6781 agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg
6841 ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg
6901 aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc
6961 gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag
7021 ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa
7081 atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt
7141 ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt
7201 caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg
7261 ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt
7321 caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta
7381 ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt
7441 taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc
7501 acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac
7561 gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag
7621 attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata
7681 gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac
7741 atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg
7801 taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta
7861 ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta
7921 tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg
7981 ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag
8041 acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatgcca
8101 ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg
8161 ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa
8221 actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa
8281 cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat
8341 tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata
8401 ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta
8461 aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt
8521 accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat
8581 atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa
8641 tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta
8701 atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc
8761 ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg
8821 ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac
8881 agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg
8941 ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt
9001 attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta
9061 aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact
9121 ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat
9181 atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac
9241 aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc
9301 attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc
9361 agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta
9421 ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac
9481 caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc
9541 ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag
9601 aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg
9661 atttgagtca gctaggaggt gactaactcg agtaaggatc tccaggcatc aaataaaacg
9721 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
9781 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata cctagggata
9841 tattccgctt cctcgctcac tgactcggtc tcggatccga gctcccgcgg gcggccgcac
9901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
9961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
10021 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
10081 tcacgaggcc ctttcgtctt caagaattga tcctctagca caaaagaaaa acgaaatgat
10141 acaccaatca gtgcaaaaaa agatataatg ggagataaga cggttcgtgt tcgtgctgac
10201 ttgcaccata tcataaaaat cgaaacagca aagaatggcg gaaacgtaaa agaagttatg
10261 gaaataagac ttagaagcaa acttaagagt gtgttgatag tgcagtatct taaaattttg
10321 tataatagga attgaagtta aattagatgc taaaaatttg taattaagaa ggagtgatta
10381 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
10441 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
10501 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
10561 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
10621 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggag
10681 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc
10741 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
10801 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
10861 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
10921 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
10981 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
11041 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
11101 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
11161 ctaaagggaa tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga
11221 ggtccctaga ctctagaccc ggggatctct gcagtgagat ctggtaatga ctctctagct
11281 tgaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
11341 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gctctagcta agcagaaggc
11401 catcctgacg gatggccttt ttgcgtttct acaaactctt gttaactcta gagctgcctg
11461 ccgcgtttcg gtgatgaaga tcttcccgat gattaattaa ttcagaacgc tcggttgccg
11521 ccgggcgttt tttatgcagc aatggcaaga acgttgctct agagcggccg catcgattca
11581 cagtggcaat ctcccccgta ttcgtttgaa atgtgccaca ttaacagcgc cgggtgatgt
11641 ccgtatcgtt ctgctaataa gcggttgatg tgccgtgttt tttctcggta gactttagat
11701 gtgaggcagt ggttgtgcct tccgccgtgc agctgtttga cgcgggaggc attgacgcgc
11761 aaaacttccg gataggtttg cgacagccag gccggacggg ctccgctcgg cgttgctaat
11821 atgacccggc cgcctatact gtgaatccgc tcaaaaatat catccagcca ttcaaattga
11881 tatacgccct cctccggctc aagtgcgctc catgcaaaaa tgccgacaga aaacgtattc
11941 gtatgagaaa gcttcatcag tttgatatcg tcagctaaaa tatcgggccg atccagccac
12001 tgatcggggt tgtagtctcc cccatggagc ataaattttg cttttgttac gtgcgttttt
12061 tcaagctttg acatcacccc
//
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