pCS2+8CeGFP

价格：1100元

联系方式：I47-825O-882O

相关技术服务：质粒构建    基因合成    质粒大提

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pCS2+8CeGFP载体质粒基本信息

出品公司: 爱迪基因
载体名称: pCS2+8CeGFP
质粒类型: 克隆载体；哺乳动物表达载体；广宿主载体；体外表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶，多克隆位点
启动子: simian CMV IE94、lac、T3、SP6
载体大小: 4854 bp
5' 测序引物及序列: SP6 fwd（MCS1）、T3 fwd（MCS2）
3' 测序引物及序列: EBV rev（MCS1）、SV40 polyA rev（MCS2）
载体标签: GFP
载体抗性: 氨苄青霉素
克隆菌株: DH10B
表达细胞: 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。
备注: pCS2+8CeGFP载体能够在多种细胞中进行高水平的瞬表达。pCS2+8CeGFP载体同时也能进行体外转录和翻译。
产品目录号: #34952
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pCS2+8CeGFP质粒图谱载体图谱和pCS2+8CeGFP载体序列质粒序列多克隆位点信息

pCS2+8CeGFP载体图谱



pCS2+8CeGFP 多克隆位点1

pCS2+8CeGFP 多克隆位点2

pCS2+8CeGFP质粒载体简介

pCS2+8CeGFP质粒序列载体序列

LOCUS       pCS2+8CeGFP Sequencing Result	4854 bp 	DNA	SYN
DEFINITION  pCS2+8CeGFP Sequencing Result
ACCESSION   
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pCS2+8CeGFP|
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="pCS2+8CeGFP Sequencing Result"
                     /mol_type="other DNA"
     gene            complement(921..1781)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(921..1781)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(1823..1851)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             2347..2871
                     /label="ORF frame 1"
                     /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
                     IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
                     GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
                     LCHIVFHIWVFLLT*"
     misc_feature    3340..3381
                     /label="CMV_fwd_primer"
                     /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
                     IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
                     GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
                     LCHIVFHIWVFLLT*"
     misc_feature    3457..3474
                     /label="Sp6_primer"
                     /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
                     IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
                     GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
                     LCHIVFHIWVFLLT*"
     CDS             3561..4277
                     /label="ORF frame 3"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
     gene            3567..4274
                     /label="GFP (variant)"
                     /gene="GFP (variant)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
     misc_feature    complement(3591..3619)
                     /label="GFP_R_primer"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
     misc_feature    complement(3635..3654)
                     /label="GFPfusionREV_primer"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
     misc_feature    3741..3770
                     /label="Y66 (EGFP)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
     misc_feature    4214..4234
                     /label="GFP_F_primer"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
     misc_feature    complement(4352..4371)
                     /label="EBV_rev_primer"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
     promoter        complement(4574..4593)
                     /label="T3_promoter"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
     promoter        complement(4610..4628)
                     /label="M13_reverse_primer"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
     misc_feature    complement(4627..4649)
                     /label="M13_pUC_rev_primer"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK*"
ORIGIN
    1 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaAGGCGGTA
   61 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
  121 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
  181 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
  241 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
  301 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
  361 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
  421 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
  481 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
  541 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
  601 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
  661 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
  721 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
  781 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
  841 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
  901 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
  961 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
 1021 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT
 1081 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
 1141 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
 1201 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
 1261 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
 1321 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
 1381 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
 1441 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
 1501 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
 1561 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
 1621 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
 1681 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
 1741 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
 1801 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
 1861 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TAAATTGTAA
 1921 GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC
 1981 AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA
 2041 GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG
 2101 GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT
 2161 TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA
 2221 GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG
 2281 CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG
 2341 CGCTTAATGC GCCGCTACAG GGCGCGTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG
 2401 AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGTC GACCGCCAAT TCAATATGGC
 2461 GTATATGGAC TCATGCCAAT TCAATATGGT GGATCTGGAC CTGTGCCAAT TCAATATGGC
 2521 GTATATGGAC TCGTGCCAAT TCAATATGGT GGATCTGGAC CCCAGCCAAT TCAATATGGC
 2581 GGACTTGGCA CCATGCCAAT TCAATATGGC GGACCTGGCA CTGTGCCAAC TGGGGAGGGG
 2641 TCTACTTGGC ACGGTGCCAA GTTTGAGGAG GGGTCTTGGC CCTGTGCCAA GTCCGCCATA
 2701 TTGAATTGGC ATGGTGCCAA TAATGGCGGC CATATTGGCT ATATGCCAGG ATCAATATAT
 2761 AGGCAATATC CAATATGGCC CTATGCCAAT ATGGCTATTG GCCAGGTTCA ATACTATGTA
 2821 TTGGCCCTAT GCCATATAGT ATTCCATATA TGGGTTTTCC TATTGACGTA GATAGCCCCT
 2881 CCCAATGGGC GGTCCCATAT ACCATATATG GGGCTTCCTA ATACCGCCCA TAGCCACTCC
 2941 CCCATTGACG TCAATGGTCT CTATATATGG TCTTTCCTAT TGACGTCATA TGGGCGGTCC
 3001 TATTGACGTA TATGGCGCCT CCCCCATTGA CGTCAATTAC GGTAAATGGC CCGCCTGGCT
 3061 CAATGCCCAT TGACGTCAAT AGGACCACCC ACCATTGACG TCAATGGGAT GGCTCATTGC
 3121 CCATTCATAT CCGTTCTCAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCACTTGG
 3181 CAGTACATCA ATATCTATTA ATAGTAACTT GGCAAGTACA TTACTATTGG AAGTACGCCA
 3241 GGGTACATTG GCAGTACTCC CATTGACGTC AATGGCGGTA AATGGCCCGC GATGGCTGCC
 3301 AAGTACATCC CCATTGACGT CAATGGGGAG GGGCAATGAC GCAAATGGGC GTTCCATTGA
 3361 CGTAAATGGG CGGTAGGCGT GCCTAATGGG AGGTCTATAT AAGCAATGCT CGTTTAGGGA
 3421 ACCGCCATTC TGCCTGGGGA CGTCGGAGCA AGCTTGATTT AGGTGACACT ATAGAATACA
 3481 AGCTACTTGT TCTTTTTGCA GGATCCACTA GTGGCGCGCC ATTAATTAAA GGCCGGCCAG
 3541 CGATCGCCGG ACATCGATTG ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA
 3601 TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG
 3661 AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC
 3721 CTGTTCCATG GCCAACACTT GTCACTACTC TCACTTATGG TGTTCAATGC TTTTCAAGAT
 3781 ACCCAGATCA TATGAAACGG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC
 3841 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT
 3901 TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG
 3961 GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG
 4021 CAGACAAACA AAAGAATGGA ATCAAAGTTA ACTTCAAAAT TAGACACAAC ATTGAAGATG
 4081 GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC
 4141 TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA
 4201 AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG
 4261 ATGAACTATA CAAATAAGAA TTCAAGGCCT CTCGAGCCTC TAGAACTATA GTGAGTCGTA
 4321 TTACGTAGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT
 4381 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT
 4441 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA
 4501 GGGGGAGGTG TGGGAGGTTT TTTAATTCGC GGCCGCGGCG CCAATGCATT GGGCCCGGTA
 4561 CCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC ATGGTCATAG
 4621 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGGAGC
 4681 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC
 4741 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA
 4801 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACT

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