pSP72

LOCUS       X65332                  2462 bp    DNA     circular SYN 25-JAN-2000
DEFINITION  Cloning vector pSP72.
ACCESSION   X65332
VERSION     X65332.2  GI:6759494
KEYWORDS    beta-lactamase; bla gene; cloning vector; multiple cloning site;
            promoter.
SOURCE      Cloning vector pSP72
  ORGANISM  Cloning vector pSP72
            other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Cloning vector pSP72"
                     /mol_type="other DNA"
                     /db_xref="taxon:90137"
     regulatory      join(2446..2462,1..3)
                     /regulatory_class="promoter"
                     /note="SP6 promoter"
     misc_feature    1
                     /note="SP6 transcription initiation site"
     misc_feature    4..90
                     /note="multiple cloning sites"
     regulatory      99..118
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     misc_feature    101
                     /note="T7 transcription initiation site"
     gene            complement(1135..1995)
                     /gene="bla"
     CDS             complement(1135..1995)
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase"
                     /protein_id="CAA46432.1"
                     /db_xref="GI:58240"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 gaactcgagc agctgaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
       61 gagctcgaat tcatcgatga tatcagatct gccggtctcc ctatagtgag tcgtattaat
      121 ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      181 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     1141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2101 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     2161 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     2221 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     2281 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     2341 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga
     2401 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
     2461 ta
//