| 出品公司: | TaKaRa |
|---|---|
| 载体名称: | pHY300PLK |
| 质粒类型: | 枯草芽孢杆菌表达载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4870 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin和Tetracycline |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
pHY300PLK载体质粒基本信息
pHY300PLK质粒图谱载体图谱和pHY300PLK载体序列质粒序列多克隆位点信息
pHY300PLK质粒载体简介
pHY300PLK质粒序列载体序列
LOCUS pHY300PLK 4870 bp DNA SYN
DEFINITION pHY300PLK
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4870
/organism="pHY300PLK"
/mol_type="other DNA"
CDS complement(100..1386)
/label="ORF frame 2"
/translation="MVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSD
QLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYI
PKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLK
KEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFV
DPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSV
IIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSF
TKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEV
DQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF*"
CDS complement(1683..2687)
/label="ORF frame 3"
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVD
YFELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKH
GIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK
NLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLD
YDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHR
KRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE
*"
promoter 3219..3247
/label="AmpR_promoter"
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVD
YFELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKH
GIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK
NLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLD
YDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHR
KRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE
*"
gene 3289..4149
/label="Ampicillin"
/gene="Ampicillin"
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVD
YFELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKH
GIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK
NLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLD
YDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHR
KRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE
*"
CDS 3289..4149
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 aagcttctag agatctgcag gtcgacggat ccccgggaat tcctgttata aaaaaaggat
61 caattttgaa ctctctccca aagttgatcc cttaacgatt tagaaatccc tttgagaatg
121 tttatataca ttcaaggtaa ccagccaact aatgacaatg attcctgaaa aaagtaataa
181 caaattacta tacagataag ttgactgatc aacttccata ggtaacaacc tttgatcaag
241 taagggtatg gataataaac cacctacaat tgcaatacct gttccctctg ataaaaagct
301 ggtaaagtta agcaaactca ttccagcacc agcttcctgc tgtttcaagc tacttgaaac
361 aattgttgat ataactgttt tggtgaacga aagcccacct aaaacaaata cgattataat
421 tgtcatgaac catgatgttg tttctaaaag aaaggaagca gttaaaaagc taacagaaag
481 aaatgtaact ccgatgttta acacgtataa aggacctctt ctatcaacaa gtatcccacc
541 aatgtagccg aaaataatga cactcattgt tccagggaaa ataattacac ttccgatttc
601 ggcagtactt agctggtgaa catctttcat catataagga accatagaga caaaccctgc
661 tactgttcca aatataattc ccccacaaag aactccaatc ataaaaggta tatttttccc
721 taatccggga tcaacaaaag gatctgttac tttcctgata tgttttacaa atatcaggaa
781 tgacagcacg ctaacgataa gaaaagaaat gctatatgat gttgtaaaca acataaaaaa
841 tacaatgcct acagacatta gtataattcc tttgatatca aaatgacctt ttatccttac
901 ttctttcttt aataatttca taagaaacgg aacagtgata attgttatca taggaatgag
961 tagaagatag gaccaatgaa tataatgggc tatcattcca ccaatcgctg gaccgactcc
1021 ttctcccatg gctactatcg atccaataag accaaatgct ttacccctat tttcctttgg
1081 aatatagcgc gcaactacaa ccattacgag tgctggaaat gcagctgcac cagccccttg
1141 aataaaacga gccataataa gtaaggaaaa gaaagaatgg ccaacaaacc caattaccga
1201 cccgaaacaa tttattataa ttccaaatag gagtaacctt ttgatgccta attgatcaga
1261 tagctttcca tatacagctg ttccaatgga aaaggttaac ataaaggctg tgttcaccca
1321 gtttgtactc gcaggtggtt tattaaaatc atttgcaata tcaggtaatg agacgttcaa
1381 aaccatttca tttaatacgc taaaaaaaga taaaatgcaa agccaaatta aaatttggtt
1441 gtgtcgtaaa ttcgattgtg aataggatgt attcacattt caccctccaa taatgagggc
1501 agacgtagtt tatagggtta atgatacgct tccctctttt aattgaaccc tgttacattc
1561 attattcatt acacttcata attaattcct cctaaacttg attaaaacat tttaccacat
1621 ataaactaag ttttaaattc agtatttcat cacttataca acaatatggc ccgtttgttg
1681 aactactctt taataaaata atttttccgt tcccaattcc acattgcaat aatagaaaat
1741 ccatcttcat cggctttttc gtcatcatct gtatgaatca aatcgccttc ttctgtgtca
1801 tcaaggttta attttttatg tatttctttt aacaaaccac cataggagat taacctttta
1861 cggtgtaaac cttcctccaa atcagacaaa cgtttcaaat tcttttcttc atcatcggtc
1921 ataaaatccg tatcctttac aggatatttt gcagtttcgt caattgccga ttgtatatcc
1981 gatttatatt tatttttcgg tcgaatcatt tgaactttta catttggatc atagtctaat
2041 ttcattgcct ttttccaaaa ttgaatccat tgtttttgat tcacgtagtt ttctgtattc
2101 ttaaaataag ttggttccac acataccaat acatgcatgt gctgattata agaattatct
2161 ttattattta ttgtcacttc cgttgcacgc ataaaaccaa caagattttt attaattttt
2221 ttatattgca tcattcggcg aaatccttga gccatatctg acaaactctt atttaattct
2281 tcgccatcat aaacattttt aactgttaat gtgagaaaca accaacgaac tgttggcttt
2341 tgtttaataa cttcagcaac aaccttttgt gactgaatgc catgtttcat tgctctcctc
2401 cagttgcaca ttggacaaag cctggattta caaaaccaca ctcgatacaa ctttctttcg
2461 cctgtttcac gattttgttt atactctaat atttcagcac aatcttttac tctttcagcc
2521 tttttaaatt caagaatatg cagaagttca aagtaatcaa cattagcgat tttcttttct
2581 ctccatggtc tcacttttcc actttttgtc ttgtccacta aaacccttga tttttcatct
2641 gaataaatgc tactattagg acacataata ttaaaagaaa cccccatcta tttagttatt
2701 tgtttggtca cttataactt taacagatgg ggtttttctg tgcaaccaat tttaagggtt
2761 ttccaatact ttaaaacaca tacataccaa cacttcaacg cacctttcag caactaaaat
2821 aaaaatgacg ttatttctat atgtatcaag ataagaaaga acaagttcaa aaccatcaaa
2881 aaaagacacc ttttcaggtg ctttttttat tttataaact cattccctga tctcgacttc
2941 gttctttttt tacctctcgg ttatgagtta gttcaaattc gttcttttta ggttctaaat
3001 cgtgtttttc ttggaattgt gctgttttat cctttacctt gtctacaaac cccttaaaaa
3061 cgtttttaaa ggcttttaag cgtctgtacg ttccttaagg aattattcct tagtgctttc
3121 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
3181 gtccgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
3241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
3301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
3361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
3421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
3481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
3541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
3601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
3661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
3721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
3781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
3841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
3901 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
3961 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
4021 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
4081 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
4141 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
4201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
4261 taacgtgagt tttcgttcca ctgagcgtca gaccccttaa taagatgatc ttcttgagat
4321 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggaggt
4381 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tgcaggagcg
4441 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac
4501 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt
4561 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg
4621 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
4681 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
4741 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
4801 accactgatt tgagcgtcag atttcgtgat gcttgtcagg gggcggagcc tatggaaaaa
4861 cgctttgccc
//
