| 质粒名称: | pEBTet_NAD-Snifit-mito |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 113916 |
| 存储实验室: | Kai Johnsson |
|
载体骨架基本信息 |
|
| 载体名称: | pEBTet |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 11448 bp |
| 完整质粒大小: | 14157 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | NAD-Snifit,hSPR(D41W42)-HaloTag7-Pro30-SNAPtag |
| 物种来源: | Synthetic |
| 插入大小: | 2349 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV TetO2 |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV_Fwd |
| 3' 测序引物 | BGH_Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pEBTet_NAD-Snifit-mito was a gift from Kai Johnsson (Addgene plasmid # 113916 ; http://n2t.net/addgene:113916 ; RRID:Addgene_113916) |
| 参考文献部分: | Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K. Elife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638. 32638 [pii] PubMed 29809136 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEBTet_NAD-Snifit-mito 14157 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid encoding the mitochondrial localization of the FRET
semi-synthetic biosensor measuring NAD+ concentration in mammalian
cells.
ACCESSION .
VERSION .
KEYWORDS pEBTet_NAD-Snifit-mito
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14157)
AUTHORS Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K
TITLE Semisynthetic biosensors for mapping cellular concentrations of
nicotinamide adenine dinucleotides.
JOURNAL Elife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
PUBMED 29809136
REFERENCE 2 (bases 1 to 14157)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..14157
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 12..86
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
CDS 99..170
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="SVLTPLLLRGLTGSARRLPVPRAK"
CDS 189..260
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="SVLTPLLLRGLTGSARRLPVPRAK"
CDS 273..344
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="SVLTPLLLRGLTGSARRLPVPRAK"
CDS 1167..2051
/codon_start=1
/product="modified bacterial dehalogenase that forms
covalent bonds with chloroalkane derivatives"
/label=HaloTag(R)
/translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
SEIARWLSTLEISG"
primer_bind complement(2782..2799)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2788..2899
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3440..5229
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind complement(7893..7911)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7979..8083
/gene="bla"
/label=AmpR promoter
CDS 8084..8944
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8302..8321)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9115..9703
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9604..9623
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(9892..9912)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9910..10207
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind 10139..10158
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 10284..10883
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(10284..10303)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 10780..10800
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 10974..11108
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11246..11265)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 11542..11803
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
intron 11920..12492
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind 12500..12519
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind 12547..12566
/label=T7
/note="T7 promoter, forward primer"
promoter 12547..12565
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 12576..13193
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
primer_bind complement(12649..12668)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
polyA_signal 13238..13359
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
enhancer 13414..13793
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 13794..13997
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 13948..13968
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 13999..14017
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 14020..14038
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 14048..14072
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 agcttaccac catgtccgtc ctgacgccgc tgctgctgcg gggcttgaca ggctcggccc
61 ggcggctccc agtgccgcgc gccaagatcc attcgttgtc cgtcctgacg ccgctgctgc
121 tgcggggctt gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag atccattcgt
181 tgggaggcag cgtgctcacc ccactcctgc tgcggggctt gacaggctcg gcccggcggc
241 tcccagtgcc gcgcgctaaa attcacagcc tctccgtcct gacgccgctg ctgctgcggg
301 gcttgacagg ctcggcccgg cggctcccag tgccgcgcgc caagatccat tcgttgggag
361 gcagcggagg cagcgagggc gggctggggc gtgctgtgtg cttgctgacc ggggcctccc
421 gcggcttcgg ccggacgctg gccccgctcc tggcctcgct gctgtcgccc ggctccgtgc
481 ttgtccttag cgactggaac gacgaggcac tgcgccagct ggaggccgag ctgggcgccg
541 agcggtctgg cctgcgcgtg gtgcgggtgc ccgccgacct gggcgccgag gccggcttgc
601 agcagctgct cggcgccctg cgcgagctcc cccggcccaa ggggctgcag cgactgctgc
661 ttatcaacaa cgcgggctct cttggggatg tgtccaaagg cttcgtggac ctgagtgact
721 ccactcaagt gaacaactac tgggcactga acttgacctc catgctctgc ctgacttcca
781 gcgtcctgaa ggccttcccg gacagtcctg gcctcaacag aaccgtggtt aacatctcgt
841 ccctctgtgc cctgcaacct ttcaaaggct gggcgctgta ctgtgcagga aaggctgctc
901 gtgatatgct gttccaggtc ctggcgctgg aggaacctaa tgtgagggtg ctgaactatg
961 ccccaggtcc tctggacaca gacatgcagc agttggcccg ggagacctcc gtggacccag
1021 acatgcgaaa agggctgcag gagctgaagg caaaggggaa gctggtggat tgcaaggtgt
1081 cagcccagaa actgctgagc ttactggaaa aggacgagtt caagtctgga gcccacgtgg
1141 acttctatga caaagaattc ggatccgaaa tcggtactgg ctttccattc gacccccatt
1201 atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc
1261 ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc
1321 atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca
1381 aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag
1441 ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc
1501 actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc
1561 ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca
1621 ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc
1681 cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc
1741 tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg
1801 agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg
1861 tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc
1921 gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc
1981 tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg tctactctgg
2041 agatttccgg tagcggccgc cctcctcctc cgccaccgcc accaccgccg ccgccgccac
2101 caccacctcc tccgcctcct ccaccaccac cgccaccgcc tccaccacct ggcggccgct
2161 ctcggtctct cgagatggat aaagactgcg aaatgaaacg cactaccctg gacagcccgc
2221 tgggtaaact ggaactgagc ggttgcgagc agggcctgca cgaaatcatt ttcctgggca
2281 aaggtacgag cgctgcggat gcggtcgaag taccggctcc ggctgcggtt ctgggtggcc
2341 cggagccgct gatgcaggct accgcatggc tgaacgcata ttttcaccag ccagaggcga
2401 ttgaagagtt ccctgtaccg gccctgcatc acccggtatt tcagcaggaa tcctttaccc
2461 gtcaggtact gtggaaactg ctgaaagtag tgaaattcgg cgaggtgatc tcctattccc
2521 atctggcggc gctggctggc aacccagcgg caactgcggc ggtgaaaacc gctctgtctg
2581 gtaatccggt gccaattctg atcccgtgcc accgtgtggt tcagggcgac ctggacgtag
2641 gtggctacga aggcggcctg gctgtgaaag aatggctgct ggcgcacgaa ggtcaccgtc
2701 tgggtaaacc aggcctgggc taaagctagc ggcgcgcccg gccgctcgag tctagagggc
2761 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
2821 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
2881 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
2941 tggggcagga cagcaagggg gaggattggg aagacaatag caggctagag gatcgatccc
3001 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
3061 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
3121 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
3181 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
3241 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
3301 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
3361 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
3421 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
3481 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
3541 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
3601 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
3661 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
3721 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
3781 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
3841 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
3901 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
3961 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
4021 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
4081 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
4141 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
4201 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
4261 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
4321 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
4381 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
4441 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
4501 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
4561 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
4621 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
4681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
4741 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
4801 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
4861 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
4921 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
4981 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
5041 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
5101 gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg
5161 agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc
5221 cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc taaagccgtc
5281 gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg
5341 agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg ttgggaggac
5401 gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga tgtctactgg
5461 ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg gcgtctccta
5521 acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct tcatctccgt
5581 catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac cagggaggca
5641 aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta ggagcgggct
5701 ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata tgagtttgta
5761 aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt gggccgggtc
5821 caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt ggaatagcaa
5881 gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat acgaacacac
5941 cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc aggagagctc
6001 ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca cgatgctttt
6061 ccaaaccacc ctcctttttt gcgccctgcc tccatcaccc tgaccccggg gtccagtgct
6121 tgggccttct cctgggtcat ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg
6181 ctcaccatct gggccacctt cttggtggta ttcaaaataa tcggcttccc ctacagggtg
6241 gaaaaatggc cttctacctg gagggggcct gcgcggtgga gacccggatg atgatgactg
6301 actactggga ctcctgggcc tcttttctcc acgtccacga cctctccccc tggctctttc
6361 acgacttccc cccctggctc tttcacgtcc tctaccccgg cggcctccac tacctcctcg
6421 accccggcct ccactacctc ctcgaccccg gcctccactg cctcctcgac cccggcctcc
6481 acctcctgct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc
6541 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc
6601 ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
6661 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
6721 tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc
6781 ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
6841 ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc
6901 tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc
6961 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
7021 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc
7081 tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
7141 tcctgctcct gcccctcctg ctcctgcccc tcccgctcct gctcctgctc ctgttccacc
7201 gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga caccatctct
7261 atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg
7321 tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc cactgccgcc
7381 ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag gtcctgtacc
7441 tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt tattagacga
7501 cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc ccaccctcat
7561 ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc tccgaaccat
7621 cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca tcctcaagat
7681 ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg ctggacggta
7741 gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac agagatgcta
7801 ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc gatgataagc
7861 tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
7921 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
7981 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
8041 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
8101 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
8161 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
8221 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
8281 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
8341 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
8401 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
8461 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
8521 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
8581 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
8641 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
8701 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
8761 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
8821 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
8881 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
8941 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
9001 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
9061 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
9121 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
9181 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
9241 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
9301 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
9361 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
9421 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
9481 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
9541 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
9601 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
9661 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
9721 ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac
9781 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
9841 cataatgggg aaggccatcc agcctcgcgt cgaactagat gatccggctg tggaatgtgt
9901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
9961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
10021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
10081 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
10141 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
10201 tttttggagg gtgaccgcca cgaccggtgc cgccaccatc ccctgaccca cgcccctgac
10261 ccctcacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc
10321 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
10381 cgcgccacac cgtcgacccc gaccgccaca tcgaacgcgt caccgagctg caagaactct
10441 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
10501 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
10561 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
10621 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
10681 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
10741 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
10801 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
10861 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
10921 gcgcacgacc cggtccgacg cggcccgacg ggtccgaggg gggtcgacct cgaaacttgt
10981 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
11041 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
11101 tctggatctg atccgaaccc cttcctcgac caattctcat gtttgacagc ttatcatcgc
11161 agatccgggc aacgttgttg ccattgctcg accaattctc atgtttgaca gcttatcatc
11221 gcagatccgt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
11281 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
11341 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
11401 cgctgcttcg cgatgtacgg gccagatata cgcgtatctg aggggactag ggtgtgttta
11461 ggcgaaaagc ggggcttcgg ttgtacgcgg ttaggagtcc cctcaggata tagtagtttc
11521 gcttttgcat agggaggggg aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat
11581 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
11641 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
11701 gattggacga accactgaat tccgcattgc agagatattg tatttaagtg cctagctcga
11761 tacaataaac gccatttgac cattcaccac attggtgtgc acctccaagc tggccgggga
11821 tcctctaggg cctctgagct attccagaag tagtgaagag gcttttttgg aggcctaggc
11881 ttttgcaaaa agctccggat cgatcctgag aacttcaggg tgagtttggg gacccttgat
11941 tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg
12001 gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt
12061 ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct
12121 gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt
12181 tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata
12241 ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga
12301 atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac
12361 atcctggtca tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg
12421 aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc
12481 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
12541 gaattgtaat acgactcact atagggcgaa ttgatatgtc tagattagat aaaagtaaag
12601 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
12661 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
12721 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
12781 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
12841 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
12901 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
12961 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
13021 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
13081 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
13141 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt
13201 acagcggatc ccgggaattc agatcttatt aaagcagaac ttgtttattg cagcttataa
13261 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
13321 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt cgaatccatg
13381 gcgcagaact ggtaggtatg gaagatccgc gttgacattg attattgact agttattaat
13441 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
13501 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
13561 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
13621 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
13681 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
13741 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
13801 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
13861 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
13921 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
13981 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc
14041 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
14101 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaactta
//
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