| 质粒名称: | GW1-Peredox-mCherry-NLS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32381 |
| 存储实验室: | Gary Yellen |
|
载体骨架基本信息 |
|
| 载体名称: | GW1 |
| 载体出品公司: | British Biotechnology |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5300 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Peredox-mCherry-NLS |
| 插入大小: | 2800 bp |
| 启动子 | CMV |
| 标签/融合蛋白: | Nuclear Localization Seq（C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | PspOMI(未破坏) |
| 3' 克隆位点: | SalI(未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | GW1-Peredox-mCherry-NLS was a gift from Gary Yellen (Addgene plasmid # 32381) |
| 参考文献部分: | Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS GW1-Peredox-mCherry-NLS 8075 bp DNA SYN
DEFINITION GW1-Peredox-mCherry-NLS
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8075
/organism="GW1-Peredox-mCherry-NLS"
/mol_type="other DNA"
CDS 121..2919
/label="ORF frame 1"
CDS complement(934..1485)
/label="ORF frame 3"
CDS complement(1683..2246)
/label="ORF frame 1"
CDS complement(1978..2940)
/label="ORF frame 3"
gene 2182..2886
/label="mCherry"
/gene="mCherry"
misc_feature 2359..2412
/label="Q66M (mCherry)"
misc_feature 2887..2907
/label="SV40 NLS"
misc_feature 2893..2907
/label="NLS"
terminator 3044..3175
/label="SV40_PA_terminator"
misc_feature 3132..3151
/label="EBV_rev_primer"
promoter complement(3195..3213)
/label="M13_reverse_primer"
misc_feature complement(3212..3234)
/label="M13_pUC_rev_primer"
promoter complement(3248..3277)
/label="lac_promoter"
rep_origin complement(3586..4205)
/label="pBR322_origin"
gene complement(4360..5220)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4360..5220)
/label="ORF frame 3"
promoter complement(5299..5327)
/label="AmpR_promoter"
misc_feature complement(5486..5508)
/label="pGEX_3_primer"
misc_feature complement(5695..5714)
/label="SV40pro_F_primer"
misc_feature complement(5745..5888)
/label="lacZ_a"
CDS complement(5745..6272)
/label="ORF frame 1"
misc_feature 5859..5881
/label="M13_pUC_fwd_primer"
promoter 5874..5890
/label="M13_forward20_primer"
promoter 6440..7016
/label="CMV_immearly_promoter"
misc_feature 6519..6806
/label="CAG_enhancer"
misc_feature 6973..6993
/label="CMV_fwd_primer"
promoter 6974..7043
/label="CMV_promoter"
promoter 6986..7105
/label="CMV2_promoter"
misc_feature 7017..7036
/label="pCEP_fwd_primer"
misc_feature 7019..7043
/label="LNCX_primer"
CDS complement(7511..7969)
/label="ORF frame 2"
CDS complement(7872..762)
/label="ORF frame 3"
ORIGIN
1 tccgagtccg ataccgataa agattctgaa tcggagtgag tgccgcgtcc gagagcgttc
61 cctgtccacg tccaccatcg gcggagcagg tgtgcctgag gcctctagat tgggcccacc
121 atggttgcta gcaaagttcc tgaagcagcc atttccagac tgattactta cctgagaatt
181 ctggaagagc tggaagccca aggtgtacac cgcaccgcct ccgagcaact gggcgagctg
241 gcccaggtca ccgccttcca ggttgataag gacctgtcct acttcggctc ctacggcact
301 gacggcgttg gctacactgt accggtcctc aagagagaac tgcgccatat cctcggtctg
361 aaccgcaaat ggggcctgtg catcgtgggg atgggccgcc tgggatccgc tcttgctgat
421 tggcctggtt ttggcgagag cttcgagctg cgcggtttct tcgatgttga cccaggtatg
481 gttggtcggc cggttcgcgg tggtgttatc gaacacgttg atctgctgcc ccagcgcgta
541 cctggtcgca tcgaaatcgc tctgctgacg gttccacgcg aggcagcaca gaaggctgcc
601 gacctgctgg ttgccgctgg catcaagggc atcctgaact tcgctccagt tgtactggag
661 gttcccaagg aagtggctgt tgagaacgtt gacatcctgg ccggtctgac gcgtctgagc
721 tttgccattc tgaaccccac gtggagcgca gcaggtgggc atggtttcac tgctcacaac
781 gtgtatatca tggctgacaa acagaagaac ggtatcaagg ccaacttcaa gatccgccac
841 aacatcgaag acggcggcgt tcagctggct gatcactacc agcagaacac cccaatcggc
901 gacggccctg ttctgctgcc tgacaaccac tacttaagca tccagtctaa actgtccaaa
961 gacccaaacg agaagcgcga ccacatggtt ctgctggaat ttgttaccgc cgctggcatc
1021 acccacggca tggacgaact gtacaagggc ggcaccgggg gatctatggt ttccaaaggc
1081 gaggaactgt tcactggtgt tgttcctatc ctggttgaac tggacggcga cgttaacggc
1141 cacaaattca gcgttagcgg ggaaggcgaa ggagacgcta cctacggcaa gctgactctg
1201 aagttcatct gcaccactgg caagctgcct gttccttggc ctaccctggt tactaccttc
1261 tcctatggtg ttatggtttt cgccagatat cctgaccaca tgaagcagca cgacttcttc
1321 aagagcgcca tgcctgaggg ctacgttcag gaacgcacca tctttttcaa ggacgacggc
1381 aactataaga cacgcgctga ggttaagttc gagggcgaca ctctggttaa ccgcatcgag
1441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccataagct tgaatataac
1501 accaaggtac ctgaagccgc catcagccgc ctcatcactt acctccgcat cctggaggag
1561 ctcgaagccc aaggcgttca ccgcactgca agcgagcagc tgggcgaact ggcacaggtt
1621 acagcattcc aggttgacga agatctgtcc tatttcggtt cctacggcac cgacggtgtt
1681 ggctacacag tccctgtcct gaagcgcgag ctgcgccaca tcctgggcct gaaccgcaag
1741 tggggcttat gcatcgttgg catgggccgc ctcggtagtg cactggcaga ctggccgggc
1801 tttggtgaat ctttcgaact gcgcggcttc ttcgacgttg atccaggcat ggttgggcgc
1861 cctgttcgcg gtggcgttat cgagcatgtt gacctcctgc cacagcgcgt cccgggtcgc
1921 atcgagatcg ccctgctgac cgttcctcgc gaagctgcac agaaggcagc cgacttacta
1981 gtcgccgctg gtatcaaggg aattctgaac ttcgcccctg ttgttctgga ggttcctaag
2041 gaagttgctg ttgaaaacgt tgatttcctg gccggcctga cccgcctgag cttcgcaatc
2101 ctgaatccta agtggcgcga agaaatgatg ggttccggaa ctgggggcaa cgcctctgac
2161 ggtggtgggt ctggtggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
2221 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
2281 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
2341 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
2401 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
2461 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
2521 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
2581 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
2641 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
2701 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
2761 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
2821 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgaactg
2881 tacaagccta aaaagaaacg taaagttgaa gatgcttgag tcgacgaatt cattgatcat
2941 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
3001 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
3061 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
3121 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgtac
3181 cgagctcgcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
3241 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
3301 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
3361 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
3421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
3481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
3541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
3601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
3661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
3721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
3781 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
3841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
3901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
3961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
4021 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
4081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
4141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
4201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
4261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
4321 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
4381 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
4441 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
4501 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
4561 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
4621 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
4681 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
4741 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
4801 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
4861 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
4921 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
4981 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
5041 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
5101 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
5161 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
5221 actcttcctt tttcaataag cggccgcggc catgccggcc actagtctcg agttattatt
5281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
5341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
5401 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
5461 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
5521 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
5581 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
5641 atatgaagac gtcgcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
5701 cctctgcata aataaaaaaa attagtcagc gcgccattcg ccattcaggc tgcgcaactg
5761 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
5821 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
5881 gacggccagt gccatctgca gtgaataata aaatgtgtgt ttgtccgaaa tacgcgtttg
5941 agatttctgt cccgactaaa ttcatgtcgc gcgatagtgg tgtttatcgc cgatagagat
6001 ggcgatattg gaaaaatcga tatttgaaaa tatggcatat tgaaaatgtc gccgatgtga
6061 gtttctgtgt aactgatatc gccatttttc caaaagttga tttttgggca tacgcgatat
6121 ctggcgatac gcttatatcg tttacggggg atggcgatag acgcctttgg tgacttgggc
6181 gattctgtgt gtcgcaaata tcgcagtttc gatataggtg acagacgata tgaggctata
6241 tcgccgatag aggcgacatc aagctggcac atggccaatg catatcgatc tatacattga
6301 atcaatattg gccattagcc atattattca ttggttatat agcataaatc aatattggct
6361 attggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
6421 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
6481 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
6541 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
6601 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
6661 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
6721 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
6781 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
6841 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
6901 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
6961 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
7021 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
7081 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc
7141 cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt
7201 cttatgcatg ctatactgtt tttggcttgg ggtctataca cccccgcttc ctcatgttat
7261 aggtgatggt atagcttagc ctataggtgt gggttattga ccattattga ccactcccct
7321 attggtgacg atactttcca ttactaatcc ataacatggc tctttgccac aactctcttt
7381 attggctata tgccaataca ctgtccttca gagactgaca cggactctgt atttttacag
7441 gatggggtct catttattat ttacaaattc acatatacaa caccaccgtc cccagtgccc
7501 gcagttttta ttaaacataa cgtgggatct ccacgcgaat ctcgggtacg tgttccggac
7561 atgggctctt ctccggtagc ggcggagctt ctacatccga gccctgctcc catgcctcca
7621 gcgactcatg gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca
7681 gcacgatgcc caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg
7741 aaaatgagct cggggagcgg gcttgcaccg ctgacgcatt tggaagactt aaggcagcgg
7801 cagaagaaga tgcaggcagc tgagttgttg tgttctgata agagtcagag gtaactcccg
7861 ttgcggtgct gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc
7921 gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct
7981 gcagtcaccg tccttgacac gaagcttccc ggccacagca atcgatccat gacacaacca
8041 gtgacaagca gtcccccagt gaaaccgcat ccgca
//
