pcDNA3.1-Peredox-mCherry-NLS

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载体基本信息

质粒名称: pcDNA3.1-Peredox-mCherry-NLS 
出品公司: 爱迪基因
目录编号: 32381
存储实验室: Gary Yellen

载体骨架基本信息
载体名称: pcDNA3.1
载体出品公司: InVitrogen
载体抗性: 氨苄霉素
空载体大小: 5400 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: Peredox-mCherry-NLS
插入大小: 2800 bp
启动子 CMV
标签/融合蛋白: Nuclear Localization Seq(C端)
克隆方法: 限制性内切酶
5' 克隆位点: XbaI(未破坏)
3' 克隆位点: XhoI(克隆时被破坏)
5' 测序引物 T7
3' 测序引物 mCherry-R

文献引用方法
材料和方法部分: pcDNA3.1-Peredox-mCherry-NLS was a gift from Gary Yellen (Addgene plasmid # 32384)
参考文献部分: Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714

载体图谱质粒图谱和多克隆位点信息

pcDNA3.1-Peredox-mCherry-NLS载体图谱

载体简介

载体序列

LOCUS       pcDNA3.1-Peredox-mCherry-NLS	8155 bp 	DNA	SYN
DEFINITION  pcDNA3.1-Peredox-mCherry-NLS
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="pcDNA3.1-Peredox-mCherry-NLS"
                     /mol_type="other DNA"
     CDS             complement(28..762)
                     /label="ORF frame 2"
     promoter        66..84
                     /label="T7_promoter"
     CDS             121..2919
                     /label="ORF frame 1"
     CDS             complement(934..1485)
                     /label="ORF frame 2"
     CDS             complement(1683..2246)
                     /label="ORF frame 3"
     gene            2182..2886
                     /label="mCherry"
                     /gene="mCherry"
     misc_feature    2359..2412
                     /label="Q66M (mCherry)"
     misc_feature    2887..2907
                     /label="SV40 NLS"
     misc_feature    2893..2907
                     /label="NLS"
     misc_feature    complement(2952..2969)
                     /label="BGH_rev_primer"
     terminator      2955..3182
                     /label="bGH_PA_terminator"
     rep_origin      3245..3551
                     /label="f1_origin"
     misc_feature    complement(3665..3685)
                     /label="pBABE_3_primer"
     misc_feature    complement(3671..3886)
                     /label="SV40_enhancer"
     promoter        3683..3951
                     /label="SV40_promoter"
     rep_origin      3850..3927
                     /label="SV40_origin"
     misc_feature    3912..3931
                     /label="SV40pro_F_primer"
     CDS             4066..4860
                     /label="ORF frame 1"
     gene            4069..4857
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(4375..4911)
                     /label="ORF frame 2"
     terminator      5037..5156
                     /label="SV40_PA_terminator"
     misc_feature    5125..5144
                     /label="EBV_rev_primer"
     promoter        complement(5200..5218)
                     /label="M13_reverse_primer"
     misc_feature    complement(5217..5239)
                     /label="M13_pUC_rev_primer"
     promoter        complement(5253..5282)
                     /label="lac_promoter"
     gene            complement(6362..7222)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(6362..7222)
                     /label="ORF frame 1"
     promoter        complement(7264..7292)
                     /label="AmpR_promoter"
     promoter        7594..55
                     /label="CMV_immearly_promoter"
     misc_feature    7673..7960
                     /label="CAG_enhancer"
     misc_feature    8127..8147
                     /label="CMV_fwd_primer"
ORIGIN
    1 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
   61 gaaattaata cgactcacta tagggagacc caagctggct agctctagat tgggcccacc
  121 atggttgcta gcaaagttcc tgaagcagcc atttccagac tgattactta cctgagaatt
  181 ctggaagagc tggaagccca aggtgtacac cgcaccgcct ccgagcaact gggcgagctg
  241 gcccaggtca ccgccttcca ggttgataag gacctgtcct acttcggctc ctacggcact
  301 gacggcgttg gctacactgt accggtcctc aagagagaac tgcgccatat cctcggtctg
  361 aaccgcaaat ggggcctgtg catcgtgggg atgggccgcc tgggatccgc tcttgctgat
  421 tggcctggtt ttggcgagag cttcgagctg cgcggtttct tcgatgttga cccaggtatg
  481 gttggtcggc cggttcgcgg tggtgttatc gaacacgttg atctgctgcc ccagcgcgta
  541 cctggtcgca tcgaaatcgc tctgctgacg gttccacgcg aggcagcaca gaaggctgcc
  601 gacctgctgg ttgccgctgg catcaagggc atcctgaact tcgctccagt tgtactggag
  661 gttcccaagg aagtggctgt tgagaacgtt gacatcctgg ccggtctgac gcgtctgagc
  721 tttgccattc tgaaccccac gtggagcgca gcaggtgggc atggtttcac tgctcacaac
  781 gtgtatatca tggctgacaa acagaagaac ggtatcaagg ccaacttcaa gatccgccac
  841 aacatcgaag acggcggcgt tcagctggct gatcactacc agcagaacac cccaatcggc
  901 gacggccctg ttctgctgcc tgacaaccac tacttaagca tccagtctaa actgtccaaa
  961 gacccaaacg agaagcgcga ccacatggtt ctgctggaat ttgttaccgc cgctggcatc
 1021 acccacggca tggacgaact gtacaagggc ggcaccgggg gatctatggt ttccaaaggc
 1081 gaggaactgt tcactggtgt tgttcctatc ctggttgaac tggacggcga cgttaacggc
 1141 cacaaattca gcgttagcgg ggaaggcgaa ggagacgcta cctacggcaa gctgactctg
 1201 aagttcatct gcaccactgg caagctgcct gttccttggc ctaccctggt tactaccttc
 1261 tcctatggtg ttatggtttt cgccagatat cctgaccaca tgaagcagca cgacttcttc
 1321 aagagcgcca tgcctgaggg ctacgttcag gaacgcacca tctttttcaa ggacgacggc
 1381 aactataaga cacgcgctga ggttaagttc gagggcgaca ctctggttaa ccgcatcgag
 1441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccataagct tgaatataac
 1501 accaaggtac ctgaagccgc catcagccgc ctcatcactt acctccgcat cctggaggag
 1561 ctcgaagccc aaggcgttca ccgcactgca agcgagcagc tgggcgaact ggcacaggtt
 1621 acagcattcc aggttgacga agatctgtcc tatttcggtt cctacggcac cgacggtgtt
 1681 ggctacacag tccctgtcct gaagcgcgag ctgcgccaca tcctgggcct gaaccgcaag
 1741 tggggcttat gcatcgttgg catgggccgc ctcggtagtg cactggcaga ctggccgggc
 1801 tttggtgaat ctttcgaact gcgcggcttc ttcgacgttg atccaggcat ggttgggcgc
 1861 cctgttcgcg gtggcgttat cgagcatgtt gacctcctgc cacagcgcgt cccgggtcgc
 1921 atcgagatcg ccctgctgac cgttcctcgc gaagctgcac agaaggcagc cgacttacta
 1981 gtcgccgctg gtatcaaggg aattctgaac ttcgcccctg ttgttctgga ggttcctaag
 2041 gaagttgctg ttgaaaacgt tgatttcctg gccggcctga cccgcctgag cttcgcaatc
 2101 ctgaatccta agtggcgcga agaaatgatg ggttccggaa ctgggggcaa cgcctctgac
 2161 ggtggtgggt ctggtggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
 2221 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
 2281 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
 2341 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
 2401 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
 2461 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
 2521 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
 2581 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
 2641 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
 2701 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
 2761 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
 2821 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgaactg
 2881 tacaagccta aaaagaaacg taaagttgaa gatgcttgag tcgagaagct taagtttaaa
 2941 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
 3001 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
 3061 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
 3121 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
 3181 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
 3241 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
 3301 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
 3361 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
 3421 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
 3481 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
 3541 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
 3601 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
 3661 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
 3721 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
 3781 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
 3841 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
 3901 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
 3961 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
 4021 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
 4081 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
 4141 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
 4201 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
 4261 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
 4321 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
 4381 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
 4441 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
 4501 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
 4561 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
 4621 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
 4681 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
 4741 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
 4801 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
 4861 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
 4921 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
 4981 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
 5041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
 5101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
 5161 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
 5221 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
 5281 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
 5341 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
 5401 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
 5461 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
 5521 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
 5581 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
 5641 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
 5701 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
 5761 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
 5821 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
 5881 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
 5941 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
 6001 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
 6061 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
 6121 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
 6181 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
 6241 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
 6301 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
 6361 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
 6421 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
 6481 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
 6541 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
 6601 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
 6661 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
 6721 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
 6781 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
 6841 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
 6901 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
 6961 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
 7021 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
 7081 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
 7141 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
 7201 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
 7261 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
 7321 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
 7381 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
 7441 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
 7501 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
 7561 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
 7621 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
 7681 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
 7741 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
 7801 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
 7861 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
 7921 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
 7981 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
 8041 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
 8101 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgg

//

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