| 质粒名称: | pcDNA3.1-Peredox-mCherry-NLS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32381 |
| 存储实验室: | Gary Yellen |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | InVitrogen |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5400 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Peredox-mCherry-NLS |
| 插入大小: | 2800 bp |
| 启动子 | CMV |
| 标签/融合蛋白: | Nuclear Localization Seq（C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI(未破坏) |
| 3' 克隆位点: | XhoI(克隆时被破坏) |
| 5' 测序引物 | T7 |
| 3' 测序引物 | mCherry-R |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3.1-Peredox-mCherry-NLS was a gift from Gary Yellen (Addgene plasmid # 32384) |
| 参考文献部分: | Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA3.1-Peredox-mCherry-NLS 8155 bp DNA SYN
DEFINITION pcDNA3.1-Peredox-mCherry-NLS
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8155
/organism="pcDNA3.1-Peredox-mCherry-NLS"
/mol_type="other DNA"
CDS complement(28..762)
/label="ORF frame 2"
promoter 66..84
/label="T7_promoter"
CDS 121..2919
/label="ORF frame 1"
CDS complement(934..1485)
/label="ORF frame 2"
CDS complement(1683..2246)
/label="ORF frame 3"
gene 2182..2886
/label="mCherry"
/gene="mCherry"
misc_feature 2359..2412
/label="Q66M (mCherry)"
misc_feature 2887..2907
/label="SV40 NLS"
misc_feature 2893..2907
/label="NLS"
misc_feature complement(2952..2969)
/label="BGH_rev_primer"
terminator 2955..3182
/label="bGH_PA_terminator"
rep_origin 3245..3551
/label="f1_origin"
misc_feature complement(3665..3685)
/label="pBABE_3_primer"
misc_feature complement(3671..3886)
/label="SV40_enhancer"
promoter 3683..3951
/label="SV40_promoter"
rep_origin 3850..3927
/label="SV40_origin"
misc_feature 3912..3931
/label="SV40pro_F_primer"
CDS 4066..4860
/label="ORF frame 1"
gene 4069..4857
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(4375..4911)
/label="ORF frame 2"
terminator 5037..5156
/label="SV40_PA_terminator"
misc_feature 5125..5144
/label="EBV_rev_primer"
promoter complement(5200..5218)
/label="M13_reverse_primer"
misc_feature complement(5217..5239)
/label="M13_pUC_rev_primer"
promoter complement(5253..5282)
/label="lac_promoter"
gene complement(6362..7222)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(6362..7222)
/label="ORF frame 1"
promoter complement(7264..7292)
/label="AmpR_promoter"
promoter 7594..55
/label="CMV_immearly_promoter"
misc_feature 7673..7960
/label="CAG_enhancer"
misc_feature 8127..8147
/label="CMV_fwd_primer"
ORIGIN
1 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
61 gaaattaata cgactcacta tagggagacc caagctggct agctctagat tgggcccacc
121 atggttgcta gcaaagttcc tgaagcagcc atttccagac tgattactta cctgagaatt
181 ctggaagagc tggaagccca aggtgtacac cgcaccgcct ccgagcaact gggcgagctg
241 gcccaggtca ccgccttcca ggttgataag gacctgtcct acttcggctc ctacggcact
301 gacggcgttg gctacactgt accggtcctc aagagagaac tgcgccatat cctcggtctg
361 aaccgcaaat ggggcctgtg catcgtgggg atgggccgcc tgggatccgc tcttgctgat
421 tggcctggtt ttggcgagag cttcgagctg cgcggtttct tcgatgttga cccaggtatg
481 gttggtcggc cggttcgcgg tggtgttatc gaacacgttg atctgctgcc ccagcgcgta
541 cctggtcgca tcgaaatcgc tctgctgacg gttccacgcg aggcagcaca gaaggctgcc
601 gacctgctgg ttgccgctgg catcaagggc atcctgaact tcgctccagt tgtactggag
661 gttcccaagg aagtggctgt tgagaacgtt gacatcctgg ccggtctgac gcgtctgagc
721 tttgccattc tgaaccccac gtggagcgca gcaggtgggc atggtttcac tgctcacaac
781 gtgtatatca tggctgacaa acagaagaac ggtatcaagg ccaacttcaa gatccgccac
841 aacatcgaag acggcggcgt tcagctggct gatcactacc agcagaacac cccaatcggc
901 gacggccctg ttctgctgcc tgacaaccac tacttaagca tccagtctaa actgtccaaa
961 gacccaaacg agaagcgcga ccacatggtt ctgctggaat ttgttaccgc cgctggcatc
1021 acccacggca tggacgaact gtacaagggc ggcaccgggg gatctatggt ttccaaaggc
1081 gaggaactgt tcactggtgt tgttcctatc ctggttgaac tggacggcga cgttaacggc
1141 cacaaattca gcgttagcgg ggaaggcgaa ggagacgcta cctacggcaa gctgactctg
1201 aagttcatct gcaccactgg caagctgcct gttccttggc ctaccctggt tactaccttc
1261 tcctatggtg ttatggtttt cgccagatat cctgaccaca tgaagcagca cgacttcttc
1321 aagagcgcca tgcctgaggg ctacgttcag gaacgcacca tctttttcaa ggacgacggc
1381 aactataaga cacgcgctga ggttaagttc gagggcgaca ctctggttaa ccgcatcgag
1441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccataagct tgaatataac
1501 accaaggtac ctgaagccgc catcagccgc ctcatcactt acctccgcat cctggaggag
1561 ctcgaagccc aaggcgttca ccgcactgca agcgagcagc tgggcgaact ggcacaggtt
1621 acagcattcc aggttgacga agatctgtcc tatttcggtt cctacggcac cgacggtgtt
1681 ggctacacag tccctgtcct gaagcgcgag ctgcgccaca tcctgggcct gaaccgcaag
1741 tggggcttat gcatcgttgg catgggccgc ctcggtagtg cactggcaga ctggccgggc
1801 tttggtgaat ctttcgaact gcgcggcttc ttcgacgttg atccaggcat ggttgggcgc
1861 cctgttcgcg gtggcgttat cgagcatgtt gacctcctgc cacagcgcgt cccgggtcgc
1921 atcgagatcg ccctgctgac cgttcctcgc gaagctgcac agaaggcagc cgacttacta
1981 gtcgccgctg gtatcaaggg aattctgaac ttcgcccctg ttgttctgga ggttcctaag
2041 gaagttgctg ttgaaaacgt tgatttcctg gccggcctga cccgcctgag cttcgcaatc
2101 ctgaatccta agtggcgcga agaaatgatg ggttccggaa ctgggggcaa cgcctctgac
2161 ggtggtgggt ctggtggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
2221 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
2281 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
2341 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
2401 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
2461 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
2521 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
2581 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
2641 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
2701 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
2761 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
2821 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgaactg
2881 tacaagccta aaaagaaacg taaagttgaa gatgcttgag tcgagaagct taagtttaaa
2941 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
3001 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
3061 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
3121 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
3181 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
3241 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
3301 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
3361 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
3421 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
3481 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
3541 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
3601 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
3661 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
3721 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
3781 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
3841 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
3901 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
3961 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
4021 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
4081 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
4141 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
4201 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
4261 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
4321 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
4381 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
4441 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
4501 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
4561 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
4621 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
4681 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
4741 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
4801 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
4861 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
4921 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
4981 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
5041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
5101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
5161 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
5221 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
5281 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
5341 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
5401 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
5461 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
5521 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
5581 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
5641 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
5701 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
5761 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
5821 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
5881 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
5941 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
6001 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
6061 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
6121 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
6181 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
6241 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
6301 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
6361 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
6421 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
6481 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
6541 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
6601 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
6661 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
6721 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
6781 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
6841 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
6901 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
6961 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
7021 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
7081 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
7141 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
7201 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
7261 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
7321 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
7381 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
7441 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
7501 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
7561 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
7621 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
7681 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
7741 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
7801 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
7861 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
7921 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
7981 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
8041 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
8101 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgg
//
