| 质粒名称: | pLenti-MP2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 36097 |
| 存储实验室: | Pantelis Tsoulfas |
|
载体骨架基本信息 |
|
| 载体名称: | pRRLsinPPT-CMV-MCS-WPRE |
| 载体出品公司: | Naldini Lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6782 bp |
| 完整质粒大小: | -- |
| 载体修饰: | new polylinker (MP2) |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti-MP2 was a gift from Pantelis Tsoulfas (Addgene plasmid # 36097 ; http://n2t.net/addgene:36097 ; RRID:Addgene_36097) |
| 参考文献部分: | A multifunctional neurotrophin with reduced affinity to p75NTR enhances transplanted Schwann cell survival and axon growth after spinal cord injury. Enomoto M, Bunge MB, Tsoulfas P. Exp Neurol. 2013 Oct;248:170-82. doi: 10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20. 10.1016/j.expneurol.2013.06.013 PubMed 23792206 |
买家导航
pLentiMP2载体基本信息
pLentiMP2载体图谱质粒图谱和多克隆位点信息
pLentiMP2载体简介
pLentiMP2载体序列
LOCUS pLenti-MP2 6782 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION 3rd generation lentiviral plasmid.
ACCESSION .
VERSION .
KEYWORDS pLenti-MP2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6782)
AUTHORS Enomoto M, Bunge MB, Tsoulfas P
TITLE A multifunctional neurotrophin with reduced affinity to p75NTR
enhances transplanted Schwann cell survival and axon growth after
spinal cord injury.
JOURNAL Exp Neurol. 2013 Oct;248:170-82. doi:
10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20.
PUBMED 23792206
REFERENCE 2 (bases 1 to 6782)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6782
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 3..229
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 230..410
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 457..582
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1075..1308
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 1804..1920
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 1943..2246
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2247..2450
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2401..2421
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2447..2471
/label=LNCX
/note="Human CMV promoter, forward primer"
misc_feature 2628..3216
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2681..2701)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3099..3110)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3303..3536
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3608..3729
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3645..3664)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3699..3718
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 3769..3904
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3831..3850
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3924..3943)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(3925..3943)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3953..3970)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(3953..3969)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3962..3984)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 4111..4566
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4198..4217)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4408..4429
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 4592..4696
/gene="bla"
/label=AmpR promoter
CDS 4697..5557
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4915..4934)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5728..6316
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6217..6236
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6470..6487
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 6604..6625
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6640..6670
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6678..6694
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6683..6705
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 6702..6718
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6702..6718
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 6737..6757
/label=T3
/note="T3 promoter, forward primer"
promoter 6739..6757
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
1921 atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
2461 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgcc
2521 cgggcgtacg ggatcccacc ggtgctcgag atgcatacta gtgaattcac gcgttctaga
2581 cctgcagggt cgactccgga tgatcagggc cctgtacaga tatcgacaat caacctctgg
2641 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
2701 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
2761 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
2821 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
2881 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
2941 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
3001 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
3061 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
3121 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
3181 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt
3241 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
3301 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
3361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
3421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
3481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
3541 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
3601 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
3661 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
3721 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
3781 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
3841 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
3901 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
3961 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
4021 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
4081 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
4141 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
4201 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
4261 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
4321 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
4381 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
4441 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
4501 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
4561 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
4621 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
4681 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
4741 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
4801 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
4861 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
4921 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
4981 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
5041 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
5101 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
5161 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
5221 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
5281 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
5341 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
5401 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
5461 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
5521 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
5581 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
5641 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
5701 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
5761 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
5821 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
5881 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
5941 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
6001 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
6061 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
6121 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
6181 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
6241 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
6301 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
6361 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
6421 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
6481 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
6541 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
6601 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
6661 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
6721 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
6781 tt
//
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pLentiMP2载体质粒应用举例
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