| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pIZT/V5-His |
| 质粒类型: | 昆虫表达载体；荧光报告载体 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 启动子: | OplE2 |
| 载体大小: | 3336 bp |
| 5' 测序引物及序列: | OpIE2 Forward：CGCAACGATCTGGTAAACAC |
| 3' 测序引物及序列: | -- |
| 载体标签: | V5 Epitope Tag , His Tag (C-端) |
| 载体抗性: | Zeocin |
| 真核筛选标记: | Zeocin；GFP |
| 克隆菌株: | TOP10；DH5alpha |
| 宿主细胞（系）: | Sf9, High Five |
| 备注: |
pIZT/V5-His载体含有荧光报告基因GFP，与抗性基因Zeocin融合表达，即Zeocin-GFP融合蛋白的形式； 表达水平高。 |
| 产品目录号: | V8010-01 |
| 瞬表达/稳表达: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pIZT/V5-His载体质粒基本信息
pIZT/V5-His质粒图谱载体图谱和pIZT/V5-His载体序列质粒序列多克隆位点信息
pIZT/V5-His质粒载体简介
The InsectSelect Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression
• The Zeocin resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistance protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells.
Promoter: OplE2
Vector Type: pIZ
Product Size: 1 kit
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: V5 Epitope Tag, His Tag (6x)
Protein Tag Position (to your gene): C-terminal
pIZT/V5-His质粒序列载体序列
LOCUS pIZT/V5-His 3336 bp DNA SYN
DEFINITION pIZT/V5-His
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3336
/organism="pIZT/V5-His"
/mol_type="other DNA"
misc_feature 515..534
/label="OpIE2_fwd_primer"
rep_origin complement(991..1610)
/label="pBR322_origin"
promoter 1984..2051
/label="EM7_promoter"
CDS 2052..3143
/label="ORF frame 3"
gene 2076..2771
/label="GFP (0 - 695)"
/gene="GFP (0 - 695)"
misc_feature complement(2100..2128)
/label="GFP_R_primer"
misc_feature complement(2144..2163)
/label="GFPfusionREV_primer"
misc_feature 2723..2743
/label="GFP_F_primer"
gene 2781..3140
/label="bleo"
/gene="bleo"
misc_feature 2781..3143
/label="sh_ble"
terminator 3210..5
/label="SV40_PA_terminator"
misc_feature 3298..3317
/label="EBV_rev_primer"
ORIGIN
1 GGATCATGAT GATAAACAAT GTATGGTGCT AATGTTGCTT CAACAACAAT TCTGTTGAAC
61 TGTGTTTTCA TGTTTGCCAA CAAGCACCTT TATACTCGGT GGCCTCCCCA CCACCAACTT
121 TTTTGCACTG CAAAAAAACA CGCTTTTGCA CGCGGGCCCA TACATAGTAC AAACTCTACG
181 TTTCGTAGAC TATTTTACAT AAATAGTCTA CACCGTTGTA TACGCTCCAA ATACACTACC
241 ACACATTGAA CCTTTTTGCA GTGCAAAAAA GTACGTGTCG GCAGTCACGT AGGCCGGCCT
301 TATCGGGTCG CGTCCTGTCA CGTACGAATC ACATTATCGG ACCGGACGAG TGTTGTCTTA
361 TCGTGACAGG ACGCCAGCTT CCTGTGTTGC TAACCGCAGC CGGACGCAAC TCCTTATCGG
421 AACAGGACGC GCCTCCATAT CAGCCGCGCG TTATCTCATG CGCGTGACCG GACACGAGGC
481 GCCCGTCCCG CTTATCGCGC CTATAAATAC AGCCCGCAAC GATCTGGTAA ACACAGTTGA
541 ACAGCATCTG TTCGAATTTA AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT
601 GGAATTCTGC AGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGCGGTTCG
661 AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC
721 ACCATCACCA TTGAGTTTAT CTGACTAAAT CTTAGTTTGT ATTGTCATGT TTTAATACAA
781 TATGTTATGT TTAAATATGT TTTTAATAAA TTTTATAAAA TAATTTCAAC TTTTATTGTA
841 ACAACATTGT CCATTTACAC ACTCCTTTCA AGCGCGTGGG ATCGATGCTC ACTCAAAGGC
901 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
961 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
1021 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
1081 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
1141 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
1201 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
1261 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
1321 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
1381 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
1441 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
1501 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
1561 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
1621 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC GAAACACGCA
1681 CGGCGCGCGC ACGCAGCTTA GCACAAACGC GTCGTTGCAC GCGCCCACCG CTAACCGCAG
1741 GCCAATCGGT CGGCCGGCCT CATATCCGCT CACCAGCCGC GTCCTATCGG GCGCGGCTTC
1801 CGCGCCCATT TTGAATAAAT AAACGATAAC GCCGTTGGTG GCGTGAGGCA TGTAAAAGGT
1861 TACATCATTA TCTTGTTCGC CATCCGGTTG GTATAAATAG ACGTTCATGT TGGTTTTTGT
1921 TTCAGTTGCA AGTTGGCTGC GGCGCGCGCA GCACCTTTGC CGGGATCTGC CGGGCTGCAG
1981 CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA
2041 GGAACTAAAC CATGGCGGTA GAAAAAATGG CTAGCAAAGG AGAAGAACTT TTCACTGGAG
2101 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG
2161 GAGAGGGTGA AGGTGATGCT ACATACGGAA AGCTTACCCT TAAATTTATT TGCACTACTG
2221 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTTT CTCTTATGGT GTTCAATGCT
2281 TTTCCCGTTA TCCGGATCAT ATGAAACGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG
2341 GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAACTACAAG ACGCGTGCTG
2401 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATCGTATCGA GTTAAAAGGT ATTGATTTTA
2461 AAGAAGATGG AAACATTCTC GGACACAAAC TCGAGTACAA CTATAACTCA CACAATGTAT
2521 ACATCACGGC AGACAAACAA AAGAATGGAA TCAAAGCTAA CTTCAAAATT CGTCACAACA
2581 TTGAAGATGG ATCCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG
2641 GCCCTGTCCT TTTACCAGAC AACCATTACC TGTCGACACA ATCTGCCCTT TCGAAAGATC
2701 CCAACGAAAA GCGTGACCAC ATGGTCCTTC TTGAGTTTGT AACTGCTGCT GGGATTACAC
2761 ATGGCATGGA TGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG
2821 GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT
2881 TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG
2941 TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT
3001 GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG
3061 GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT
3121 TCGTGGCCGA GGAGCAGGAC TGACCGACGC CGACCAACAC CGCCGGTCCG ACGCGGCCCG
3181 ACGGGTCCGA GGGGGGTCGA CCTCGAAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT
3241 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG
3301 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCT
//
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pIZT/V5-His质粒载体应用举例
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