| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pMIB/V5-His B |
| 质粒类型: | 昆虫细胞表达系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | OpIE2 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3600bp |
| 5' 测序引物及序列: | OpIE2-F: 5'd[CGCAACGATCTGGTAAACAC]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | HBM (Nterm), V5 (Cterm), His (Cterm) |
| 载体抗性: | Ampicillin |
| 筛选标记: | Blasticidin |
| 备注: | 在昆虫细胞中能够稳定表达，HBM标签可实现目的蛋白的分泌表达 |
| 产品目录号: | V803001 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pMIB/V5-His B载体 pMIB-V5-HisB质粒基本信息
pMIB/V5-His B质粒图谱 pMIB-V5-HisB载体图谱和pMIB/V5-His B载体序列 pMIB-V5-HisB质粒序列多克隆位点信息
pMIB/V5-His B质粒 pMIB-V5-HisB载体简介
pMIB/V5-His B质粒序列 pMIB-V5-HisB载体序列
LOCUS pMIB/V5-His B 3600 bp DNA SYN
DEFINITION pMIB/V5-His B
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3600
/organism="pMIB/V5-His B"
/mol_type="other DNA"
misc_feature 511..530
/label="OpIE2_fwd_primer"
rep_origin complement(1066..1685)
/label="pBR322_origin"
promoter 2059..2126
/label="EM7_promoter"
gene 2127..2525
/label="blast (variant)"
/gene="blast (variant)"
gene 2645..3505
/label="Ampicillin"
/gene="Ampicillin"
CDS 2645..3505
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG
61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT
121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC
181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC
241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC
301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT
361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA
421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC
481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG
541 CATCTGTTCG AATTTAAAGC TACCATGAAA TTCTTAGTCA ACGTTGCCCT TGTTTTTATG
601 GTCGTATACA TTTCTTACAT CTATGCCGGC ATGCTAAGCT TGGTACCGAG CTCGGATCCA
661 CTAGTCCAGT GTGGTGGAAT TCTGCAGATA TCCAGCACAG TGGCGGCCGC TCGAGTCTAG
721 AGGGCCCGCG GTTCGAAGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGC
781 GTACCGGTCA TCATCACCAT CACCATTGAG TTTATCTGAC TAAATCTTAG TTTGTATTGT
841 CATGTTTTAA TACAATATGT TATGTTTAAA TATGTTTTTA ATAAATTTTA TAAAATAATT
901 TCAACTTTTA TTGTAACAAC ATTGTCCATT TACACACTCC TTTCAAGCGC GTGGGATCGA
961 TGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
1021 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
1081 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
1141 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
1201 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
1261 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
1321 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
1381 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
1441 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
1501 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
1561 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
1621 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
1681 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
1741 CATGCGAAAC ACGCACGGCG CGCGCACGCA GCTTAGCACA AACGCGTCGT TGCACGCGCC
1801 CACCGCTAAC CGCAGGCCAA TCGGTCGGCC GGCCTCATAT CCGCTCACCA GCCGCGTCCT
1861 ATCGGGCGCG GCTTCCGCGC CCATTTTGAA TAAATAAACG ATAACGCCGT TGGTGGCGTG
1921 AGGCATGTAA AAGGTTACAT CATTATCTTG TTCGCCATCC GGTTGGTATA AATAGACGTT
1981 CATGTTGGTT TTTGTTTCAG TTGCAAGTTG GCTGCGGCGC GCGCAGCACC TTTGCCGGGA
2041 TCTGCCGGGC TGCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT
2101 AATACGACAA GGTGAGGAAC TAAACCATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC
2161 TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG
2221 CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA
2281 CTGGGGGACC TTGCGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG GCAGCTGGCA
2341 ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC CCCTGCGGAC
2401 GGTGCCGACA GGTTCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG AAGGACAGTG
2461 ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG
2521 GCTAAGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTCC CGGGAGATCT GCATGTCTAC
2581 TAAACTCACA AATTAGAGCT TCAATTTAAT TATATCAGTT ATTACCCATT GAAAAAGGAA
2641 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT
2701 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG
2761 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG
2821 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT
2881 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA
2941 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA
3001 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC
3061 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG
3121 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC
3181 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT
3241 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT
3301 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG
3361 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT
3421 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG
3481 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT
3541 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT
//
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