出品公司: | Invitrogen |
---|---|
载体名称: | pFBDM |
质粒类型: | 昆虫细胞表达系统载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: |
Polyhedrin,p10
|
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5279bp |
5' 测序引物及序列: |
p2Bac-F (for MCS1):ggacctttaattcaacccaacac
Polyhedrin-F (for MCS2):AAATGATAACCATCTCGC
|
3' 测序引物及序列: |
TK-pA-R (for MCS1):TTGTCTCCTTCCGTGTTTCA
SV40pA-R (for MCS2):GAAATTTGTGATGCTATTGC
|
Genbank: | DJ417502.1 |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
筛选标记: | 庆大霉素 |
备注: | 可同时表达两种蛋白 |
产品目录号: | -- |
稳定性: | 稳定 |
组成型: | 非组成型 |
病毒/非病毒: | 杆状病毒 |
买家导航
pFBDM载体质粒基本信息
pFBDM质粒图谱载体图谱和pFBDM载体序列质粒序列多克隆位点信息
pFBDM质粒载体简介
pFBDM质粒序列载体序列
LOCUS pFBDM 5279 bp DNA circular SYN DEFINITION pFBDM ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..5279 /organism="pFBDM" /mol_type="other DNA" rep_origin 119..425 /label="f1_origin" promoter 619..647 /label="AmpR_promoter" gene 689..1549 /label="Ampicillin" /gene="Ampicillin" CDS 689..1549 /label="ORF frame 2" rep_origin 1704..2323 /label="pBR322_origin" gene complement(2902..3435) /label="gent" /gene="gent" CDS complement(2902..3435) /label="ORF frame 3" CDS 3537..4235 /label="ORF frame 3" terminator complement(4016..4286) /label="TK_PA_terminator" promoter 4521..4609 /label="PH_promoter" misc_feature 4535..4552 /label="polyhedrin_fwd_primer" terminator 4872..5003 /label="SV40_PA_terminator" misc_feature 4960..4979 /label="EBV_rev_primer" ORIGIN 1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga 61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg 2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa 2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga 2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact 2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg 2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc 2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac 2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc 3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag 3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat 3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc 3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta 3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag 3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat 3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt 3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga 3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc 3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc 3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag 3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct 3961 atagttctag tggttggcct acagctttgt ttaaacaaag ctgtacccgt agtggctatg 4021 gcagggcttg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggt 4081 tggggtgggg aaaaggaaga aacgcgggcg tattggtccc aatggggtct cggtggggta 4141 tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc 4201 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc 4261 cttccgtgtt tcagttagcc tcccccatct cccggtaccg catgctatgc atcagctgct 4321 agcaccatgg ctcgagatcc cgggtgatca agtcttcgtc gagtgattgt aaataaaatg 4381 taatttacag tatagtattt taattaatat acaaatgatt tgataataat tcttatttaa 4441 ctataatata ttgtgttggg ttgaattaaa ggtccgtata ctagtatcga ttcgcgacct 4501 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat 4561 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt 4621 attcataccg tcccaccatc gggcgcggat cccggtccga agcgcgcgga attcaaaggc 4681 ctacgtcgac gagctcacta gtcgcggccg ctttcgaatc tagagcctgc agtctcgaca 4741 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac 4801 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 4861 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 4921 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 4981 atgtatctta tcatgtctgg atctgatcac tgcttgagcc taggagatcc gaaccagata 5041 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct 5101 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc 5161 cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta 5221 tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctacttt //
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