LOCUS pHPRT-DRGFP 12467 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting
sequences.
ACCESSION .
VERSION .
KEYWORDS pHPRT-DRGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12467)
AUTHORS Pierce AJ, Hu P, Han M, Ellis N, Jasin M
TITLE Ku DNA end-binding protein modulates homologous repair of
double-strand breaks in mammalian cells.
JOURNAL Genes Dev. 2001 Dec 15. 15(24):3237-42.
PUBMED 11751629
REFERENCE 2 (bases 1 to 12467)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12467
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 21..125
/gene="bla"
/label=AmpR promoter
CDS 126..986
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(344..363)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1157..1745
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1646..1665
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1899..1916
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2033..2054
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2069..2099
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2107..2123
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2131..2147
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2168..2186
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 2223..2239
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2223..2239
/label=pBluescriptSK
/note="For pBluescript vector"
enhancer 3565..3944
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3947..4222
/label=chicken beta-actin promoter
intron 4223..5231
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 5155..5177
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 5239..5258
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 5316..5336
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5358..5378
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
regulatory 5592..5601
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind 6251..6272
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 6440..6561
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6477..6496)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6531..6550
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
CDS complement(7222..7821)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7305..7325)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 7802..7821
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
promoter complement(7840..8337)
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(7888..7907)
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
primer_bind 8279..8302
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind complement(8425..8444)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 8490..8545
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(8544..8563)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
CDS 8907..8927
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8949..8969
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
regulatory 9183..9192
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(9710..9726)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 9734..9750
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9758..9788)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9803..9824
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin 9929..10064
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9991..10010
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(11823..11842)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(11824..11842)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(11849..11866)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(11849..11865)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11858..11880)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 12007..12462
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(12094..12113)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 12304..12325
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
2161 agctcgaaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
2221 gctctagaac tagtggatcc gcggccgcta attcgattct tctgtaaaat tatcttatta
2281 gtaagttgta accatctcag acttctaagt ttgagtttgt aaaggatcag ttttttttgg
2341 tgacctacac acacacacac acacacacac acacacacac acacaggggg gtggattctg
2401 agaatttaga gaatttagat ggttaatacc agtttttatt tttattctgt gtgatattta
2461 tttattaaga tttatttatt tattacatgt aggtacactg tatctgtctt cagacacacc
2521 agaagagggc atcagatctc gttacagatg gttgtgagtc accatgtggt tgctgggatt
2581 tgaactcagg acctctggaa gaacagtcag tgctcttaac cgttgagcca tctcaccagc
2641 cccgtgatat tttatttaac ttactagaat atactgtgtt aaaacatatt ttaggggatt
2701 ggggagatgg cccattctgt aaagtgtctg ccatgtcatc ttcaggacct gcgtttgtaa
2761 ccccagcatg gagaagacag agacagggag acccctgggc ttcctggcaa gctagactag
2821 caaatttggt taactaggtt caatgagaga ctcagtctca aaacataaga agtgaatttt
2881 ttagacccaa tttcaatctc tgaactccat acgtatgcac atgtacctgc atataaatat
2941 atatacacac ccataaacac atgcacacac aaaaaatggt tcaaaagttc ttaaaatgta
3001 taagagtaga aagtgtagag ccgggagtgg tggcgcacgc ctttaatccc agcacttggg
3061 agcagaggca ggtggatttc tgagttcgag gccagcctgg tctacagagt gagttccagg
3121 acagccagag ctatacagag aaatcctgtc tcaaaaaacc aaaaataaag tgtatatact
3181 aatctttcat gcctaaaata tttatttttg ccacattttt catacctctt taatattttt
3241 ctttttctct ttgtgcttca aaggaaattt tgtgtcatgt tttacttttc attcttgact
3301 ctattcagca gtaagacgca gcatggcttt cttagagaag tataatctat tacaaaacgg
3361 taacatttaa ctgctctaca taccctcttg tagctaaatt cctctgttaa actaagactt
3421 attatctaaa cgtaattaaa tgttgatatt attgaagcat ttatcagggt tattgtctca
3481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
3541 ttccccgaaa agtgccacct ggtcgacatt gattattgac tagttattaa tagtaatcaa
3601 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
3661 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
3721 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
3781 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
3841 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
3901 ctacttggca gtacatctac gtattagtca tcgctattac catgggtcga ggtgagcccc
3961 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
4021 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg
4081 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
4141 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
4201 aaagcgaagc gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg
4261 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
4321 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc
4381 ttttctgtgg ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga
4441 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct
4501 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc
4561 gaggggagcg cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag
4621 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc
4681 tgtaaccccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
4741 gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
4801 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
4861 ggccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
4921 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg
4981 gaggcgccgc cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag
5041 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag
5101 cctcggggct gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
5161 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
5221 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
5281 aattcagatc cgccgccact atgggatcaa gatcgccaaa aaagaagaga aaggtgccga
5341 agaagcatgc agcaccacca aaaaaaaaac gaaaagtaga agacccacga ggcaacacca
5401 gcggcgtgct gagcaccccc aaggccaaga gggccaagca cccccccggc accgagaagc
5461 ccaggagcag gagccagagc gagcagcccg ccacctgccc catctgctac gccgtgatca
5521 ggcagagcag gaacctgagg aggcacctgg agctgaggca cttcgccaag cccggcgtgg
5581 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
5641 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggctagggat
5701 aacagggtaa tacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
5761 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
5821 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
5881 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
5941 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
6001 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
6061 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
6121 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
6181 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
6241 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
6301 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
6361 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
6421 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
6481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
6541 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatctgaag cttctgatgg
6601 aattagaact tggcaaaaca atactgagaa tgaagtgtat gtggaacaga ggctgctgat
6661 ctcgttcttc aggctatgaa actgacacat ttggaaacca cagtacttag aaccacaaag
6721 tgggaatcaa gagaaaaaca atgatcccac gagagatcta tagatctata gatcatgagt
6781 gggaggaatg agctggccct taatttggtt ttgcttgttt aaattatgat atccaactat
6841 gaaacattat cataaagcaa tagtaaagag ccttcagtaa agagcaggca tttatctaat
6901 cccaccccac ccccaccccc gtagctccaa tccttccatt caaaatgtag gtactctgtt
6961 ctcacccttc ttaacaaagt atgacaggaa aaacttccat tttagtggac atctttattg
7021 tttaatagat catcaatttc gatccgctcc tgggcaccga actgcgccgc gtgttcagca
7081 gggtcggcgt gttcggtgtg tcccccgcgg tgggcctcgg gggcgggtgc ggggtcggcg
7141 gggccgcccc gggtggcttc ggtcggagcc atggggtcgt gcgctccttt cggtcgggcg
7201 ctgcgggtcg tggggcgggc gtcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg
7261 tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
7321 gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
7381 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac
7441 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
7501 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
7561 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
7621 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
7681 ggtgacccgc tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc
7741 gaacgcggcg gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac
7801 cgtgggcttg tactcggtca tggtggcggc tggatcggtc gaaaggcccg gagatgagga
7861 agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga
7921 ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc
7981 ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca
8041 aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg
8101 tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct
8161 gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg
8221 cctaccggtg gatgtggaat gtgtgaggcc agaggccact tgtgtagcgc caagtgccca
8281 gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt
8341 agaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
8401 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
8461 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
8521 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
8581 acatcagaat gagtatttgg tttagagttt ggcaacatat gccatatgct ggctgccatg
8641 aacaaaggtg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
8701 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
8761 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
8821 tctcctgact actcccagtc atagctgtcc ctcttctctt atgaagatcc ctcgacctgc
8881 agcccaagct ttagggatca agatcgccaa aaaagaagag aaaggtgccg aagaagcatg
8941 cagcaccacc aaaaaaaaaa cgaaaagtag aagacccacg aggcaacacc agcggcgtgc
9001 tgagcacccc caaggccaag agggccaagc acccccccgg caccgagaag cccaggagca
9061 ggagccagag cgagcagccc gccacctgcc ccatctgcta cgccgtgatc aggcagagca
9121 ggaacctgag gaggcacctg gagctgaggc acttcgccaa gcccggcgtg gatccaccgg
9181 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
9241 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
9301 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
9361 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
9421 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
9481 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
9541 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
9601 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
9661 agaagaacgg catcaaggtg aacttcaata aaagcttggc gtaatcatgg tcatagctgt
9721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
9781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
9841 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
9901 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
9961 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
10021 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagagc ttgtttttat
10081 ttgtatcttt aaattttatg atagatgtta tagtgtactc tcctctcccc tcccctcccc
10141 tctcctcctc agcaggatct tgttatgtag ccctaaggga tctaatactt actctgtagt
10201 ccaggctgtc cttcagctca tgagacacac catcatgctt ggcatgtttc ttgttctgaa
10261 aggtatctgg ttttaatttt ggttgagctg gtgtgttggt gtatatctgt aatctcagca
10321 caggcaagta gagacaagga ttatgattac agtccagcct gggctattta gagggattct
10381 ctttaatctt tcctcatgcc ccaaaatctt acctttggta tatgaaaaat agtctccact
10441 tctgcaaaat attgctttat gaagtaagaa ttcccttcat agagacaagg aatgtgtcct
10501 gtaaaagttt aatgtgtaag aagtatttgt tataaaagat aaatattcag aatcttcttt
10561 ttaattcctg attttatttc tataggactg aaagacttgc tcgagatgtc atgaaggaga
10621 tgggaggcca tcacattgtg gccctctgtg tgctcaaggg gggctataag ttctttgctg
10681 acctgctgga ttacattaaa gcactgaata gaaatagtga tagatccatt cctatgactg
10741 tagattttat cagactgaag agctactgtg taagtataat taacttataa ttaaaaaaat
10801 agggccattc tagttttatc tatatttttt ttaaacttgt gcaaactatg ctacataatc
10861 aacttacttg attatgtaat atatatagta atagttggaa ttactattat tacatattta
10921 tttttctgtg ctggggtaga acccagggaa ctaagtactg taataaccga gcttcattca
10981 tcctagtcat ttttaatttt tttttttttt tttgtttttt cgagacaagg tttctctgtg
11041 tagccctggc tgtcctggaa ctcactctgt agaccaggct ggcctcgaac tcagaaatct
11101 gcctgcctct gcctcccagt gctgagatta atgacgtgca ccaccactgc ctggcatttt
11161 tttaaagttt taatttgaga ccctttctca ctaacttgct cacactatcc tcgaactcat
11221 tacgtgggct ggtctagaaa tttcagtcaa cttgcctcag cttctcaagt agctgggatt
11281 acaggcaggt gctaccactt ctggccatga tcattgttgt tgttgctatt attattatta
11341 ttatttcact gatacttcat atcacaacac agaatagtaa ctgtagttca tgaccaacaa
11401 aaaagttgta cagtttctct gttgtcttat tttgatctgg attttgattt gtttcatggc
11461 actgggcagg ctaatctcaa actgcatggc tcaaatagtc cttctgtctt aatctcccat
11521 gttctggaag tataggtatc actacaccta gctgtatttt catattttaa aaaaaacgcc
11581 taagaattgc tattgaatga ggcggagaga tggctcagca gtcacaagta cttgtagagg
11641 actggggttt ggttactagc acctcaaaga tggcttacaa ttatctgtag ctccaattcc
11701 agaggatctg tgtggtaaag ctgcatgtat atgggcatgc aggtaaaact gctaaacata
11761 taaaaatagc tcagtcttaa gagaattggt cgacctcgag ggggggcccg gtacccaatt
11821 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
11881 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
11941 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
12001 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
12061 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
12121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
12181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
12241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
12301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
12361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
12421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtg
//
