LOCUS HA-AR-LBD 6424 bp ds-DNA circular SYN 25-JUN-2021
DEFINITION Mammalian expression of 1xHA-tagged human androgen receptor
trancate: AR Ligand-bindind domain (LBD).
ACCESSION .
VERSION .
KEYWORDS HA-AR-LBD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6424)
AUTHORS Liu X, Han W, Gulla S, Simon NI, Gao Y, Cai C, Yang H, Zhang X, Liu
J, Balk SP, Chen S
TITLE Protein phosphatase 1 suppresses androgen receptor ubiquitylation
and degradation.
JOURNAL Oncotarget. 2016 Jan 12;7(2):1754-64. doi: 10.18632/oncotarget.6434.
PUBMED 26636645
REFERENCE 2 (bases 1 to 6424)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 25, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6424
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 919..945
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter complement(1977..1995)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(1978..1995)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(2015..2032)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2021..2245
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2291..2719
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2378..2397)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2588..2609
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2728..2748)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2733..3062
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2913..3048
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2975..2994
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3129..3923
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3183..3202)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3793..3812
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4097..4218
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4134..4153)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4188..4207
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4267..4283)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4267..4283)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4280..4302)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4291..4307
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4315..4345)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4360..4381
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4498..4515)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4669..5257)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4749..4768)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5428..6288)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6051..6070
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6289..6393)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttccgcgg
901 ctagctgcgg ccatggtcta tccttacgac gtccctgatt acgccgttaa catcgattcc
961 ggaggtaccc gagctggatc cactagtaac ggccgccagt gtgctggaat tgtgtcacac
1021 attgaaggct atgaatgtca gcccatcttt ctgaatgtcc tggaagccat tgagccaggt
1081 gtagtgtgtg ctggacacga caacaaccag cccgactcct ttgcagcctt gctctctagc
1141 ctcaatgaac tgggagagag acagcttgta cacgtggtca agtgggccaa ggccttgcct
1201 ggcttccgca acttacacgt ggacgaccag atggctgtca ttcagtactc ctggatgggg
1261 ctcatggtgt ttgccatggg ctggcgatcc ttcaccaatg tcaactccag gatgctctac
1321 ttcgcccctg atctggtttt caatgagtac cgcatgcaca agtcccggat gtacagccag
1381 tgtgtccgaa tgaggcacct ctctcaagag tttggatggc tccaaatcac cccccaggaa
1441 ttcctgtgca tgaaagcact gctactcttc agcattattc cagtggatgg gctgaaaaat
1501 caaaaattct ttgatgaact tcgaatgaac tacatcaagg aactcgatcg tatcattgca
1561 tgcaaaagaa aaaatcccac atcctgctca agacgcttct accagctcac caagctcctg
1621 gactccgtgc agcctattgc gagagagctg catcagttca cttttgacct gctaatcaag
1681 tcacacatgg tgagcgtgga ctttccggaa atgatggcag agatcatctc tgtgcaagtg
1741 cccaagatcc tttctgggaa agtcaagccc atctatttcc acacccagtg aagcattgga
1801 aaccctattt ccccacccca gctcatgccc cctttcagat gtcttctgcc tgttataact
1861 ctgcactact cctctgcagg tcgacggatc cactagtaac ggccgccagt gtgctggaat
1921 tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg ccctattcta
1981 tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
2041 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
2101 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
2161 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
2221 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
2281 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
2341 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
2401 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
2461 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
2521 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
2581 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
2641 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
2701 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
2761 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
2821 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
2881 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
2941 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
3001 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
3061 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
3121 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
3181 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
3241 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
3301 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
3361 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
3421 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
3481 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
3541 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
3601 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
3661 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
3721 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
3781 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
3841 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
3901 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
3961 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
4021 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
4081 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
4141 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
4201 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
4261 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
4321 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
4381 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
4441 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
4501 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
4561 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
4621 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
4681 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
4741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
4801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
4861 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
4921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
4981 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
5041 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
5101 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
5161 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
5221 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
5281 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
5341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
5401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
5461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
5521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
5581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
5641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
5701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
5761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
5821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
5881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
5941 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
6001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
6061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
6121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
6181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
6241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
6301 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
6361 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
6421 cgtc
//
